Information on EC 3.5.1.89 - N-acetylglucosaminylphosphatidylinositol deacetylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.1.89
-
RECOMMENDED NAME
GeneOntology No.
N-acetylglucosaminylphosphatidylinositol deacetylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of carboxylic ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol acetylhydrolase
Involved in the second step of glycosylphosphatidylinositol (GPI) anchor formation in all eukaryotes. The enzyme appears to be composed of a single subunit (PIG-L in mammalian cells and GPI12 in yeast). In some species, the long-chain sn-1-acyl group of the phosphatidyl group is replaced by a long-chain alkyl or alk-1-enyl group.
CAS REGISTRY NUMBER
COMMENTARY hide
122191-30-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
additional information
residues Asp46 and His140 of the enzyme are important for catalysis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1R,2R)-1-O-(2-acetylamino-2-deoxy-alpha-D-glucopyranosyl)-cyclohexanediol 2-(1,2-di-O-hexadecanoyl-sn-glycerol 3-phosphate) + H2O
?
show the reaction diagram
-
-
-
-
?
1-D-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-myo-inositol 1-(1,2-di-O-hexadecanoyl-sn-glycerol 3-phosphate) + H2O
?
show the reaction diagram
-
-
-
-
?
2-acetamido-2-deoxy-alpha-D-glucopyranosyl-(1->6)-phosphatidylinositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate
show the reaction diagram
-
-
-
-
?
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate
show the reaction diagram
6-(N-butanoyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + butanoate
show the reaction diagram
6-(N-hexanoyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + hexanoate
show the reaction diagram
6-(N-isobutanoyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + isobutanoate
show the reaction diagram
6-(N-pentanoyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + pentanoate
show the reaction diagram
6-(N-propionyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + propionate
show the reaction diagram
D-GlcNAc-alpha(1->6)-D-myo-inositol-1-octadecyl phosphate + H2O
D-GlcN-alpha(1->6)-D-myo-inositol-1-octadecyl phosphate + acetate
show the reaction diagram
-
-
-
-
?
D-GlcNAc-alpha1-6-D-myo-inositol-1-HPO4-3-sn-1,2-diacylglycerol + H2O
?
show the reaction diagram
-
-
-
-
?
N,N-diethylethanaminium 1-D-2-[(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)oxy]-myo-inosityl octadecyl phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
N-acetyl-D-glucosamine-alpha-(1-6)-D-myo-inositol-1-octadecyl phosphate + H2O
D-glucosamine-alpha-(1-6)-D-myo-inositol-1-octadecyl phosphate + acetate
show the reaction diagram
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O
?
show the reaction diagram
-
involved in the formation of the glycosylphosphatidylinositol membrane anchor of the trypanosome variant-surface glycoprotein
-
-
-
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O
D-Glucosaminylphosphatidylinositol + acetate
show the reaction diagram
N-acetyl-D-glucosaminylphosphatidylinositol + H2O + H2O
D-glucosaminylphosphatidylinositol + acetate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-acetamido-2-deoxy-alpha-D-glucopyranosyl-(1->6)-phosphatidylinositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate
show the reaction diagram
-
-
-
-
?
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate
show the reaction diagram
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O
?
show the reaction diagram
-
involved in the formation of the glycosylphosphatidylinositol membrane anchor of the trypanosome variant-surface glycoprotein
-
-
-
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O
D-Glucosaminylphosphatidylinositol + acetate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2R)-1-O-(2-acetylamino-2-deoxy-alpha-D-glucopyranosyl)-cyclohexanediol 2-(1,2-di-O-hexadecanoyl-sn-glycerol 3-phosphate)
-
competitive
(1R,2R)-1-O-(2-acetylamino-2-deoxy-beta-D-glucopyranosyl)-cyclohexanediol 2-(1,2-di-O-hexadecanoyl-sn-glycerol 3-phosphate)
-
-
(1R,2R)-1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-cyclohexanediol 2-(1,2-di-O-hexadecanoyl-sn-glycerol 3-phosphate)
-
-
(1R,2R)-1-O-(2-amino-2-deoxy-beta-D-glucopyranosyl)-cyclohexanediol 2-(1,2-di-O-hexadecanoyl-sn-glycerol 3-phosphate)
-
-
(1R,2R)-2-((2-deoxy-2-[(hydroxycarbamoyl)amino]-beta-D-glucopyranosyl)oxy)cyclohexyl octadecyl phosphate
-
-
-
(1R,2R)-2-[(2-acetamido-2-deoxy-beta-D-glucopyranosyl)oxy]cyclohexyl octadecyl phosphate
-
-
(1R,2R)-2-[(2-acetylamino-2-deoxy-alpha-D-glucopyranosyl)oxy]cyclohexyl octadecyl phosphate
-
-
(1R,2R)-2-[(2-amino-2-deoxy-alpha-D-glucopyranosyl)oxy]cyclohexyl octadecyl phosphate
-
-
(1R,2R)-2-[(2-amino-2-deoxy-beta-D-glucopyranosyl)oxy]cyclohexyl octadecyl phosphate
-
-
(1R,2R,3S,4R,5R,6R)-2-([2-deoxy-2-[(hydroxycarbamoyl)amino]-alpha-D-glucopyranosyl]oxy)-3,4,5,6-tetrahydroxycyclohexyl octadecyl hydrogen phosphate
-
-
(1R,2S,3R,4R,5R)-4-(prop-2-en-1-yl)-6,8-dioxabicyclo[3.2.1]octane-2,3-diyl diacetate
-
-
(2R)-3-{[{[(1R,2R,3S,4R,5R,6R)-2-{[2-(carbamoylamino)-2-deoxy-alpha-D-glucopyranosyl]oxy}-3,4,5,6-tetrahydroxycyclohexyl]oxy}(hydroxy)phosphoryl]oxy}propane-1,2-diyl dihexadecanoate
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-
1,3,4,6-tetra-O-acetyl-2-C-allyl-2-deoxy-alpha-D-glucopyranose
-
-
1,3,4,6-tetra-O-acetyl-2-C-carboxymethyl-2-deoxy-alpha-D-glucopyranose
-
-
1,3,4,6-tetra-O-acetyl-2-deoxy-2-C-formylmethyl-alpha-Dglucopyranose
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1,5-anhydro-2-(carboxymethyl)-2-deoxy-D-glucitol
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-
1,5-anhydro-2-C-(carboxymethyl N-hydroxyamide)-2-deoxy-D-glucitol
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-
1,5-anhydro-2-C-carboxymethyl-2-deoxy-D-glucitol
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1,5-anhydro-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-3-O-phenyl-D-glucitol
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1,5-anhydro-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-D-glucitol
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-
1,5-anhydro-3-O-benzoyl-2-C-carboxymethyl-2-deoxy-D-glucitol
-
-
1,5-anhydro-3-O-benzyl-2-(carboxymethyl)-2-deoxy-D-glucitol
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-
1,5-anhydro-3-O-benzyl-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-D-glucitol
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-
1,5-anhydro-3-O-benzyl-4,6-O-benzylidene-2-(carboxymethyl)-2-deoxy-D-glucitol
-
-
1,5-anhydro-3-O-benzyl-4,6-O-benzylidene-2-[2-[(benzyloxy)amino]-2-oxoethyl]-2-deoxy-D-glucitol
-
-
1,5-anhydro-4,6-O-benzylidene-2-(carboxymethyl)-2-deoxy-D-glucitol
-
-
1,5-anhydro-4,6-O-benzylidene-2-deoxy-2-prop-2-en-1-yl-D-glucitol
-
-
1,5-anhydro-4,6-O-benzylidene-2-[2-[(benzyloxy)amino]-2-oxoethyl]-2-deoxy-D-glucitol
-
-
1-acetyl-N-[4-hydroxy-3-(hydroxycarbamoyl)phenyl]piperidine-4-carboxamide
-
40% inhibition at 1 mM
1-{1-[3-chloro-5-(trifluoromethyl)pyridin-2-yl]piperidin-4-yl}-2-hydroxyethan-1-one
-
-
2-(2,4-dichlorophenoxy)-N-hydroxyacetamide
-
-
2-(carboxymethyl)-2-deoxy-D-glucopyranose
-
-
2-C-carboxymethyl-2-deoxy-D-glucopyranose
-
-
2-deoxy-2-ureido-D-galactosylalpha1-6-D-myo-inositol-1-phosphoryl-sn-1,2-dipalmitoylglycerol
-
suicide inhibitor, 50% inihibition at 0.0002 mM
2-deoxy-2-ureido-D-glucosyl-alpha1-6-D-myo-inositol-1-phosphoryl-sn-1,2-dipalmitoylglycerol
2-deoxy-2-ureido-D-glucosyl-beta1-6-D-myo-inositol-1-phosphoryl-sn-1,2-dipalmitoylglycerol
-
50% inhibition at about 8 nM
2-deoxy-2-ureido-D-glucosylalpha1-6-D-(2-O-octyl)myo-inositol-1-phosphoryl-sn-1,2-dipalmitoylglycerol
-
50% inhibition at about 8 nM
2-deoxy-2-ureido-D-glucosylbeta1-6-D-myo-inositol-1-phosphoryl-sn-1,2-dipalmitoylglycerol
-
suicide inhibitor, 50% inihibition at 0.0002 mM
2-hydroxybenzoic acid
-
97% inhibition at 1 mM
3-[3-(4-chlorophenyl)-1,2,4-oxadiazol-5-yl]-N-hydroxypropanamide
-
-
4-(2,4-dichlorophenoxy)-N-hydroxybutanamide
-
-
4-bromo-N,2-dihydroxybenzamide
-
86% inhibition at 1 mM
4-bromo-N-hydroxybenzamide
-
9.6% inhibition at 1 mM
5-((tert-butoxycarbonyl)amino)-2-hydroxybenzoic acid
-
-
-
5-amino-N-(benzyloxy)-2-hydroxybenzamide
-
-
-
5-benzamido-N,2-dihydroxybenzamide
-
96% inhibition at 1 mM
5-[(cyclohexanecarbonyl)amino]-N,2-dihydroxybenzamide
-
99% inhibition at 1 mM
N,2-dihydroxy-5-(4-methylbenzamido)benzamide
-
62% inhibition at 1 mM
N,2-dihydroxy-5-(octadecanoylamino)benzamide
-
25% inhibition at 1 mM
N,N-diethylethanaminium (1R,2R)-2-(2-acetamido-3-hydroxypropoxy)cyclohexyl octadecyl phosphate
-
-
N,N-diethylethanaminium 1-D-2-[(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)oxy]-myo-inosityl octadecyl phosphate
-
-
N-((1R,2R)-2-[(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)oxy]cyclohexyl)octadecane-1-sulfonamide
-
-
-
N-((1R,2R)-2-[(2-acetamido-2-deoxy-beta-D-glucopyranosyl)oxy]cyclohexyl)octadecane-1-sulfonamide
-
-
-
N-(1R,2R)-2-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-cyclohexyloctadecane-1-sulphonamide
-
-
N-(1R,2R)-2-O-(2-amino-2-deoxy-beta-D-glucopyranosyl)-cyclohexyloctadecane-1-sulphonamide
-
-
N-dimethyl-D-glucosyl-phosphatidylinositol
-
0.001 mM, complete inhibition
N-hydroxy-N'-(4-methoxyphenyl)urea
-
-
N-hydroxybenzamide
-
25% inhibition at 1 mM
N-[4-hydroxy-3-(hydroxycarbamoyl)phenyl]-2H-1,3-benzodioxole-5-carboxamide
-
86% inhibition at 1 mM
N-[4-hydroxy-3-(hydroxycarbamoyl)phenyl]oxane-4-carboxamide
-
56% inhibition at 1 mM
N-[4-hydroxy-3-(hydroxycarbamoyl)phenyl][1,1'-biphenyl]-4-carboxamide
-
75% inhibition at 1 mM
phenyl 2-(carbamoylamino)-2-deoxy-1-thio-beta-D-glucopyranoside
-
-
phenyl 2-(carboxymethyl)-2-deoxy-1-thio-alpha-D-glucopyranoside
-
-
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phenyl 2-(N-aminocarbonyl)amino-2-deoxy-1-thio-beta-Dglucopyranoside
-
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phenyl 2-amino-2-deoxy-1-thio-beta-D-glucopyranoside
-
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phenyl 2-C-carboxymethyl-2-deoxy-1-thio-alpha-Dglucopyranoside
-
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phenyl 2-C-carboxymethyl-2-deoxy-1-thio-beta-Dglucopyranoside
-
-
phenyl 3,4,6-tri-O-acetyl-2-C-allyl-2-deoxy-1-thio-alpha-D-glucopyranoside
-
-
phenyl 3,4,6-tri-O-acetyl-2-C-allyl-2-deoxy-1-thio-beta-D-glucopyranoside
-
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phenyl 3,4,6-tri-O-acetyl-2-C-carboxymethyl-2-deoxy-1-thio-alpha-D-glucopyranoside
-
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phenyl 3,4,6-tri-O-acetyl-2-C-carboxymethyl-2-deoxy-1-thio-beta-D-glucopyranoside
-
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phenyl 3,4,6-tri-O-acetyl-2-deoxy-2-C-formylmethyl-1-thio-alpha-D-glucopyranoside
-
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phenyl 3,4,6-tri-O-acetyl-2-deoxy-2-C-formylmethyl-1-thio-beta-D-glucopyranoside
-
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salicylic hydroxamic acid
-
enzyme inhibitor with high ligand efficiency, proposed mode of action, overview
tert-butyl (3-((benzyloxy)carbamoyl)-4-hydroxyphenyl)carbamate
-
-
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triethylammonium 1R,2R-1-O-[2-C-(carboxymethyl N-hydroxyamide)-2-deoxy-beta-D-glucopyranosyl]-cyclohexanediol 2-(n-octadecylphosphate)
-
-
triethylammonium trans-2-(2-amino-3-hydroxypropoxy)-cyclohexyl n-octadecyl phosphate
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mg2+
-
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00137 - 0.00263
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol
0.00015 - 0.00205
N-acetyl-D-glucosaminylphosphatidylinositol
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10
1,5-anhydro-2-(carboxymethyl)-2-deoxy-D-glucitol
Trypanosoma brucei
-
IC50 above 10 mM, in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30C
10
1,5-anhydro-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-D-glucitol
Trypanosoma brucei
-
IC50 above 10 mM, in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30C
0.29
1,5-anhydro-3-O-benzyl-2-(carboxymethyl)-2-deoxy-D-glucitol
Trypanosoma brucei
-
in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30C
1.5
1,5-anhydro-3-O-benzyl-2-deoxy-2-[2-(hydroxyamino)-2-oxoethyl]-D-glucitol
Trypanosoma brucei
-
in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30C
0.3
2-(carboxymethyl)-2-deoxy-D-glucopyranose
Trypanosoma brucei
-
in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30C
10
phenyl 2-(carbamoylamino)-2-deoxy-1-thio-beta-D-glucopyranoside
Trypanosoma brucei
-
IC50 above 10 mM, in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30C
0.1 - 10
phenyl 2-(carboxymethyl)-2-deoxy-1-thio-alpha-D-glucopyranoside
-
0.063
salicylic hydroxamic acid
Trypanosoma brucei
-
pH 8.0, 37C
0.019
triethylammonium 1R,2R-1-O-[2-C-(carboxymethyl N-hydroxyamide)-2-deoxy-beta-D-glucopyranosyl]-cyclohexanediol 2-(n-octadecylphosphate)
Trypanosoma brucei
-
in 50 mM Na-HEPES pH 7.4, 25 mM KCl, 0.1 mM Tos-LysCH2Cl and 0.001 mg/ml leupeptin, at 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
solubilized enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4 - 8
-
90% of activity maximum at pH 6.4 and 8.0, solubilized enzyme
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
of Trypanosoma
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
-
monomer, predicted from amino acid sequence
66000
-
x * 66000, cytoplasmic domain MBPEhDELTATMPIG-L, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 66000, cytoplasmic domain MBPEhDELTATMPIG-L, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
10-50% v/v glycerol increases stability of solubilized enzyme
-
Solubilized enzyme loses activity upon freeze-thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Highly purified preparation is extremely labile, even when stored in 50% glycerol
-
Solubilized enzyme loses activity upon prolonged storage at 4C
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
anti-FLAG M2 gel affinity column chromatography
-
recombinant N-terminally wild-type and mutant MBP-tagged cytoplasmic catalytic domains from Escherichia coli strain TB1 by amylose affinity chromatography and removal of the tag, although the MBP tag does not significantly alter the activity of the enzyme
variant MITat 1.4, partial
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
expressed with a baculovirus vector in Sf9 cells
-
expression in Escherichia coli
recombinant expression of the GST-tagged enzyme in CHO MS2S cells that are defective de-N-acetylase and express the GPI anchored human CD55
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recombinant expression of wild-type and mutant N-terminally MBP-tagged cytoplasmic catalytic domains in Escherichia coli strain TB1
the cytoplasmic domain MBPEhDELTATMPIG-L is expressed in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D102A
site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is active in absence of metal ions, but well stimulated by metal ions
D133A
site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is active in absence of metal ions, but well stimulated by metal ions
D45A
site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is active in absence of metal ions, but well stimulated by metal ions
D46A
site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions and not stimulated by metal ions
D47A
site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions, but well stimulated by metal ions
E79A
site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is active in absence of metal ions, but well stimulated by metal ions
H140A
site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions and not stimulated by metal ions
H143A
site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions, but well stimulated by metal ions
H43A
site-directed mutagenesis of the isolated cytoplasmic catalytic domain, the mutant is only slightly active in absence of metal ions, but well stimulated by metal ions
additional information
comparison of secondary conformation of the mutant proteins, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
Show AA Sequence (122 entries)
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