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Literature summary for 3.5.1.62 extracted from
- Substrate specificity and function of acetylpolyamine amidohydrolases from Pseudomonas aeruginosa (2016), BMC Biochem., 17, 4 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PA0321, sequence comparisons | Pseudomonas aeruginosa |
| gene PA1409, sequence comparisons | Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a gene disruption transposon mutant strain OMEGAPA0321. The mutation leads to only minor effects on growth of the strains on polyamines | Pseudomonas aeruginosa |
| additional information | construction of a gene disruption transposon mutant strain OMEGAPA1409. The mutation leads to only minor effects on growth of the strains on polyamines | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michelis-Menten kinetics of isozyme APAH1 and APAH2 differ, overview | Pseudomonas aeruginosa | |
| 0.011 | - |
Boc-Lys-(Tfa)-AMC | pH 8.0, 30°C | Pseudomonas aeruginosa |  |
| 0.022 | - |
Boc-Lys-(Ac)-AMC | pH 8.0, 30°C | Pseudomonas aeruginosa | |
| 0.084 | - |
Boc-Lys-(Tfa)-AMC | pH 8.0, 30°C | Pseudomonas aeruginosa | |
| 0.223 | - |
Boc-Lys-(Ac)-AMC | pH 8.0, 30°C | Pseudomonas aeruginosa | |
| 0.284 | - |
Acetylcadaverine | pH 8.0, 30°C | Pseudomonas aeruginosa | |
| 0.411 | - |
acetylputrescine | pH 8.0, 30°C | Pseudomonas aeruginosa | |
| 0.465 | - |
Acetylcadaverine | pH 8.0, 30°C | Pseudomonas aeruginosa | |
| 0.503 | - |
acetylputrescine | pH 8.0, 30°C | Pseudomonas aeruginosa | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q9I3T5 | - |
- |
| Pseudomonas aeruginosa | Q9I6H0 | - |
- |
| Pseudomonas aeruginosa 1C | Q9I3T5 | - |
- |
| Pseudomonas aeruginosa 1C | Q9I6H0 | - |
- |
| Pseudomonas aeruginosa ATCC 15692 | Q9I3T5 | - |
- |
| Pseudomonas aeruginosa ATCC 15692 | Q9I6H0 | - |
- |
| Pseudomonas aeruginosa CIP 104116 | Q9I3T5 | - |
- |
| Pseudomonas aeruginosa CIP 104116 | Q9I6H0 | - |
- |
| Pseudomonas aeruginosa DSM 22644 | Q9I3T5 | - |
- |
| Pseudomonas aeruginosa DSM 22644 | Q9I6H0 | - |
- |
| Pseudomonas aeruginosa JCM 14847 | Q9I3T5 | - |
- |
| Pseudomonas aeruginosa JCM 14847 | Q9I6H0 | - |
- |
| Pseudomonas aeruginosa LMG 12228 | Q9I3T5 | - |
- |
| Pseudomonas aeruginosa LMG 12228 | Q9I6H0 | - |
- |
| Pseudomonas aeruginosa PRS 101 | Q9I3T5 | - |
- |
| Pseudomonas aeruginosa PRS 101 | Q9I6H0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylcadaverine + H2O | - |
Pseudomonas aeruginosa | acetate + cadaverine | - |
? | |
| acetylcadaverine + H2O | - |
Pseudomonas aeruginosa ATCC 15692 | acetate + cadaverine | - |
? | |
| acetylcadaverine + H2O | - |
Pseudomonas aeruginosa 1C | acetate + cadaverine | - |
? | |
| acetylcadaverine + H2O | - |
Pseudomonas aeruginosa PRS 101 | acetate + cadaverine | - |
? | |
| acetylcadaverine + H2O | - |
Pseudomonas aeruginosa DSM 22644 | acetate + cadaverine | - |
? | |
| acetylcadaverine + H2O | - |
Pseudomonas aeruginosa CIP 104116 | acetate + cadaverine | - |
? | |
| acetylcadaverine + H2O | - |
Pseudomonas aeruginosa LMG 12228 | acetate + cadaverine | - |
? | |
| acetylcadaverine + H2O | - |
Pseudomonas aeruginosa JCM 14847 | acetate + cadaverine | - |
? | |
| acetylputrescine + H2O | - |
Pseudomonas aeruginosa | acetate + putrescine | - |
? | |
| acetylputrescine + H2O | - |
Pseudomonas aeruginosa ATCC 15692 | acetate + putrescine | - |
? | |
| acetylputrescine + H2O | - |
Pseudomonas aeruginosa 1C | acetate + putrescine | - |
? | |
| acetylputrescine + H2O | - |
Pseudomonas aeruginosa PRS 101 | acetate + putrescine | - |
? | |
| acetylputrescine + H2O | - |
Pseudomonas aeruginosa DSM 22644 | acetate + putrescine | - |
? | |
| acetylputrescine + H2O | - |
Pseudomonas aeruginosa CIP 104116 | acetate + putrescine | - |
? | |
| acetylputrescine + H2O | - |
Pseudomonas aeruginosa LMG 12228 | acetate + putrescine | - |
? | |
| acetylputrescine + H2O | - |
Pseudomonas aeruginosa JCM 14847 | acetate + putrescine | - |
? | |
| Boc-Lys-(Ac)-AMC + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Boc-Lys-(Ac)-AMC + H2O | - |
Pseudomonas aeruginosa ATCC 15692 | ? | - |
? | |
| Boc-Lys-(Ac)-AMC + H2O | - |
Pseudomonas aeruginosa 1C | ? | - |
? | |
| Boc-Lys-(Ac)-AMC + H2O | - |
Pseudomonas aeruginosa PRS 101 | ? | - |
? | |
| Boc-Lys-(Ac)-AMC + H2O | - |
Pseudomonas aeruginosa DSM 22644 | ? | - |
? | |
| Boc-Lys-(Ac)-AMC + H2O | - |
Pseudomonas aeruginosa CIP 104116 | ? | - |
? | |
| Boc-Lys-(Ac)-AMC + H2O | - |
Pseudomonas aeruginosa LMG 12228 | ? | - |
? | |
| Boc-Lys-(Ac)-AMC + H2O | - |
Pseudomonas aeruginosa JCM 14847 | ? | - |
? | |
| Boc-Lys-(Tfa)-AMC + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
| Boc-Lys-(Tfa)-AMC + H2O | - |
Pseudomonas aeruginosa ATCC 15692 | ? | - |
? | |
| Boc-Lys-(Tfa)-AMC + H2O | - |
Pseudomonas aeruginosa 1C | ? | - |
? | |
| Boc-Lys-(Tfa)-AMC + H2O | - |
Pseudomonas aeruginosa PRS 101 | ? | - |
? | |
| Boc-Lys-(Tfa)-AMC + H2O | - |
Pseudomonas aeruginosa DSM 22644 | ? | - |
? | |
| Boc-Lys-(Tfa)-AMC + H2O | - |
Pseudomonas aeruginosa CIP 104116 | ? | - |
? | |
| Boc-Lys-(Tfa)-AMC + H2O | - |
Pseudomonas aeruginosa LMG 12228 | ? | - |
? | |
| Boc-Lys-(Tfa)-AMC + H2O | - |
Pseudomonas aeruginosa JCM 14847 | ? | - |
? | |
| N1-acetylspermidine + H2O | - |
Pseudomonas aeruginosa | spermidine + acetate | - |
? | |
| N1-acetylspermine + H2O | - |
Pseudomonas aeruginosa | spermine + acetate | - |
? | |
| N1-acetylspermine + H2O | - |
Pseudomonas aeruginosa ATCC 15692 | spermine + acetate | - |
? | |
| N1-acetylspermine + H2O | - |
Pseudomonas aeruginosa 1C | spermine + acetate | - |
? | |
| N1-acetylspermine + H2O | - |
Pseudomonas aeruginosa PRS 101 | spermine + acetate | - |
? | |
| N1-acetylspermine + H2O | - |
Pseudomonas aeruginosa DSM 22644 | spermine + acetate | - |
? | |
| N1-acetylspermine + H2O | - |
Pseudomonas aeruginosa CIP 104116 | spermine + acetate | - |
? | |
| N1-acetylspermine + H2O | - |
Pseudomonas aeruginosa LMG 12228 | spermine + acetate | - |
? | |
| N1-acetylspermine + H2O | - |
Pseudomonas aeruginosa JCM 14847 | spermine + acetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetylpolyamine amidohydrolase | - |
Pseudomonas aeruginosa |
| APAH | - |
Pseudomonas aeruginosa |
| APAH1 | - |
Pseudomonas aeruginosa |
| APAH2 | - |
Pseudomonas aeruginosa |
| AphA | - |
Pseudomonas aeruginosa |
| AphB | - |
Pseudomonas aeruginosa |
| PA0321 | - |
Pseudomonas aeruginosa |
| PA1409 | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pseudomonas aeruginosa |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the amino acids involved in the catalytic mechanism are Y312, H142, H143, D178, N185 | Pseudomonas aeruginosa |
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