Information on EC 3.5.1.62 - acetylputrescine deacetylase

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The expected taxonomic range for this enzyme is: Micrococcus luteus

EC NUMBER
COMMENTARY hide
3.5.1.62
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RECOMMENDED NAME
GeneOntology No.
acetylputrescine deacetylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-acetylputrescine + H2O = acetate + putrescine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
N-acetylputrescine acetylhydrolase
The enzyme from Micrococcus luteus also acts on N8-acetylspermidine and acetylcadaverine, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
103679-48-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain K-11
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Manually annotated by BRENDA team
strain K-11
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetylcadaverine + H2O
acetate + cadaverine
show the reaction diagram
N-acetylputrescine + H2O
acetate + putrescine
show the reaction diagram
N8-acetylspermidine + H2O
acetate + spermidine
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe3+
-
1 mM: slight activation
FeCl3
-
1 mM: slight activation
Mn2+
-
1 mM: slight activation
MnCl2
-
1 mM: slight activation
additional information
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Mg2+ 1 mM: no influence
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8-hydroxyquinoline
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very slight inhibition, 1 mM: 2% inhibition
Ca2+
-
1 mM: 6.2% inhibition
CaCl2
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1 mM: 6.2% inhibition
Cu2+
-
1 mM: 74.4% inhibition
CuCl2
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1 mM: 74.4% inhibition
iodoacetamide
-
very slight inhibition, 1 mM: 2% inhibition
Ni2+
-
1 mM: 66.8% inhibition
NiCl2
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1 mM: 66.8% inhibition
p-chloromercuribenzoic acid
-
very slight inhibition, 1 mM: 2% inhibition
additional information
-
no or very slight inhibition by -SH blocking agents
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.321
N-acetylputrescine
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-
0.377
N8-Acetylspermidine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.48
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substrate: acetylputrescine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
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substrate: acetylputrescine
9
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substrate: N8-acetylspermidine
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
115000
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gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
strain K-11, partial
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