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Literature summary for 3.5.1.19 extracted from

  • Zapata-Perez, R.; Martinez-Monino, A.B.; Garcia-Saura, A.G.; Cabanes, J.; Takami, H.; Sanchez-Ferrer, A.
    Biochemical characterization of a new nicotinamidase from an unclassified bacterium thriving in a geothermal water stream microbial mat community (2017), PLoS ONE, 12, e0181561 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.19

Cloned(Commentary)

Cloned (Comment) Organism
wild type enzyme and four mutants enzymes are produced in Escherichia coli Rosetta 2 (DE3)pLysS uncultured Acidobacteria bacterium

Inhibitors

Inhibitors Comment Organism Structure
5-bromonicotinaldehyde
-
 uncultured Acidobacteria bacterium
nicotinaldehyde
-
 uncultured Acidobacteria bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 nicotinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
0.12
-
 nicotinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
0.14
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
0.15
-
 nicotinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
0.21
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
0.21
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
0.22
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
0.23
-
 nicotinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
0.23
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
0.33
-
 Pyrazinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
0.39
-
 nicotinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
0.41
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
0.46
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
0.59
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
0.67
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
0.68
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
0.74
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
0.89
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
1.08
-
 methylnicotinate 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
1.09
-
 5-Methylnicotinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
1.21
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
1.31
-
 ethylnicotinate 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
2.89
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.59
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.84
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contains a Fe2+ ion in its metal binding site, which is coordinated by an aspartate (D67) and three histidine residues (H69, H75 and H86) uncultured Acidobacteria bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21200
-
 gel filtration uncultured Acidobacteria bacterium
23900
-
 HPLC/ESI-MS uncultured Acidobacteria bacterium

Organism

Organism UniProt Comment Textmining
uncultured Acidobacteria bacterium H5SPS2 nicotinamidase obtained from a metagenomic library fosmid clone (JFF054_F02) obtained from a geothermal water stream microbial mat community in a Japanese epithermal mine
-

Purification (Commentary)

Purification (Comment) Organism
-
 uncultured Acidobacteria bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methylnicotinamide + H2O
-
 uncultured Acidobacteria bacterium 5-methylnicotinate + NH3
-
 ?
ethylnicotinate + H2O
-
 uncultured Acidobacteria bacterium nicotinate + ethanol
-
 ?
methylnicotinate + H2O
-
 uncultured Acidobacteria bacterium nicotinate + methanol
-
 ?
nicotinamide + H2O
-
 uncultured Acidobacteria bacterium nicotinate + NH3
-
 ?
pyrazinamide + H2O
-
 uncultured Acidobacteria bacterium pyrazinoic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 21200, HPLC/ESI-MS uncultured Acidobacteria bacterium

Synonyms

Synonyms Comment Organism
UbNic
-
 uncultured Acidobacteria bacterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
 at pH 7.3 and pH 9.5 uncultured Acidobacteria bacterium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 100 40°C: about 50% of maximal activity, 100°C: about 70% of maximal activity uncultured Acidobacteria bacterium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 ammonium sulfate at 1 M is able to produce a slight increase in its Tm (86.4°C) in comparison with that found in sodium phosphate pH 7.3. The nicotinamidase reaction products, nicotinic and pyrazinoic acids improved UbNic stability, increasing its Tm to 87.7°C and 87.1°C. Nicotinaldehyde and 5-bromonicotinaldehyde, increased Tm up to 92.7°C and 85.7°C uncultured Acidobacteria bacterium
71.3
-
 melting temperature in glycine (pH 10.0) uncultured Acidobacteria bacterium
73.1
-
 melting temperature in CAPS (pH 11.0) uncultured Acidobacteria bacterium
73.1
-
 melting temperature in sodium acetate (pH 4.0) uncultured Acidobacteria bacterium
84.7
-
 melting temperature in glycine buffer pH 9.0 uncultured Acidobacteria bacterium
84.8
-
 melting temperature in sodium phosphate buffer pH 7.3 uncultured Acidobacteria bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
0.2
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
0.2
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
0.3
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
1.3
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
1.9
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
3.1
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
3.5
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
3.7
-
 ethylnicotinate 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
4.3
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
4.9
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
4.9
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
5.1
-
 nicotinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
5.8
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
7.5
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
9.6
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
10.2
-
 nicotinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
12.4
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
12.5
-
 nicotinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
13.7
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
15.5
-
 nicotinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
18.7
-
 Pyrazinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
22.7
-
 nicotinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
43.7
-
 5-Methylnicotinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
54.5
-
 methylnicotinate 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5 10
-
 uncultured Acidobacteria bacterium

pH Range

pH Minimum pH Maximum Comment Organism
6 11 pH 6.0: about 60% of maximal activity, pH 11.0: about 75% of maximal activity uncultured Acidobacteria bacterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9.5 very stable nicotinamidase from acid (pH 5.0) to basic pHs (up to pH 9.5). Its stability is higher in sodium phosphate pH 7.3 and in glycine pH 9.5, maintaining 90% activity after 24 hours of incubation at such pHs. At lower pH values, the enzyme is less stable. It maintains 75% activity in sodium acetate pH 5.0 and pH 6.0 after 24 hours uncultured Acidobacteria bacterium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00018
-
 nicotinaldehyde 37°C, pH 7.3 uncultured Acidobacteria bacterium
0.00147
-
 5-bromonicotinaldehyde 37°C, pH 7.3 uncultured Acidobacteria bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.1
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
0.2
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
0.2
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
0.3
-
 ethylnicotinate 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
1.1
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
1.4
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
2.2
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
2.8
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
2.8
-
 ethylnicotinate 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
5.2
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
14.1
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
18.5
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
20.5
-
 methylnicotinate 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
34.1
-
 Pyrazinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
35
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
39.7
-
 nicotinamide 37°C, pH 7.3, mutant enzyme W83F uncultured Acidobacteria bacterium
40.1
-
 5-Methylnicotinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
45.7
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
50.5
-
 methylnicotinate 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
51
-
 nicotinamide 37°C, pH 7.3, mutant enzyme A151C uncultured Acidobacteria bacterium
53.9
-
 5-Methylnicotinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
54.3
-
 nicotinamide 37°C, pH 7.3, mutant enzyme H75S uncultured Acidobacteria bacterium
56.7
-
 Pyrazinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium
68
-
 nicotinamide 37°C, pH 7.3, mutant enzyme H75E uncultured Acidobacteria bacterium
189.2
-
 nicotinamide 37°C, pH 7.3, wild-type enzyme uncultured Acidobacteria bacterium