Cloned (Comment) | Organism |
---|---|
- |
Pectobacterium carotovorum |
- |
Dickeya chrysanthemi |
Protein Variants | Comment | Organism |
---|---|---|
D133I | analysis of the contribution of this position on thermostability | Dickeya chrysanthemi |
D133L | analysis of the contribution of this position on thermostability | Dickeya chrysanthemi |
D133T | analysis of the contribution of this position on thermostability | Dickeya chrysanthemi |
D133V | analysis of the contribution of this position on thermostability | Dickeya chrysanthemi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
L-asparagine | mutant D133T | Dickeya chrysanthemi | |
0.097 | - |
L-asparagine | mutant D133I | Dickeya chrysanthemi | |
0.153 | - |
L-asparagine | mutant D133V | Dickeya chrysanthemi | |
0.16 | - |
L-asparagine | mutant D133L | Dickeya chrysanthemi | |
0.597 | - |
N-acetyl-L-asparagine | mutant D133V | Dickeya chrysanthemi | |
1.008 | - |
L-glutamine | mutant D133T | Dickeya chrysanthemi | |
1.099 | - |
L-glutamine | mutant D133I | Dickeya chrysanthemi | |
1.677 | - |
L-glutamine | mutant D133L | Dickeya chrysanthemi | |
1.788 | - |
N-acetyl-L-asparagine | mutant D133L | Dickeya chrysanthemi | |
1.999 | - |
L-glutamine | mutant D133V | Dickeya chrysanthemi | |
2.096 | - |
N-acetyl-L-asparagine | mutant D133T | Dickeya chrysanthemi | |
5.613 | - |
N-acetyl-L-asparagine | mutant D133I | Dickeya chrysanthemi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dickeya chrysanthemi | Q6Q4F3 | - |
- |
Pectobacterium carotovorum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pectobacterium carotovorum |
- |
Dickeya chrysanthemi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartic acid + NH3 | - |
? | |
L-asparagine + H2O | - |
Dickeya chrysanthemi | L-aspartic acid + NH3 | - |
? | |
L-glutamine + H2O | - |
Pectobacterium carotovorum | L-glutamic acid + NH3 | - |
? | |
L-glutamine + H2O | - |
Dickeya chrysanthemi | L-glutamic acid + NH3 | - |
? | |
N-acetyl-L-asparagine + H2O | - |
Dickeya chrysanthemi | N-acetyl-L-aspartic acid + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
asparaginase | - |
Pectobacterium carotovorum |
asparaginase | - |
Dickeya chrysanthemi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
46.4 | - |
wild type, half-inactivation temperature, 7.5 min | Dickeya chrysanthemi |
50 | - |
mutant D133V, half-inactivation after 159.7 h | Dickeya chrysanthemi |
50 | - |
wild type, half-inactivation after 2.7 h | Dickeya chrysanthemi |
55 | - |
mutant D133V, after 7.5 min 87.3% remaining activity | Dickeya chrysanthemi |
55 | - |
wild type, after 7.5 min 19.9% remaining activity | Pectobacterium carotovorum |
55 | - |
wild type, after 7.5 min 37.2% remaining activity | Dickeya chrysanthemi |
55.8 | - |
mutant D133V, half-inactivation temperature, 7.5 min | Dickeya chrysanthemi |