Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 mM, reduces activity to 2.8% | Vipera ammodytes ammodytes | |
EDTA | 5 mM, inhibits azocaseinolytic activity | Vipera ammodytes ammodytes | |
L-cysteine | 5 mM, reduces activity to 52% | Vipera ammodytes ammodytes | |
additional information | antiserum raised against ammodytagin is able to completely neutralise the hemorrhagic activity of the whole venom | Vipera ammodytes ammodytes | |
Zn2+ | 5 mM, inhibits azocaseinolytic activity | Vipera ammodytes ammodytes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, slightly enhances activity for 19.2% | Vipera ammodytes ammodytes | |
Mg2+ | 5 mM, slightly enhances activity for 16.9% | Vipera ammodytes ammodytes | |
Zn2+ | Zn2+-dependent enzyme | Vipera ammodytes ammodytes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
1 * 50000 + 1 * 60000, SDS-PAGE under reducing conditions | Vipera ammodytes ammodytes |
60000 | - |
1 * 50000 + 1 * 60000, SDS-PAGE under reducing conditions | Vipera ammodytes ammodytes |
108000 | - |
MALDI mass spectrometry | Vipera ammodytes ammodytes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vipera ammodytes ammodytes | P0DJE2 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | total carbohydrate content is approximately 7.1% | Vipera ammodytes ammodytes |
Purification (Comment) | Organism |
---|---|
- |
Vipera ammodytes ammodytes |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Vipera ammodytes ammodytes | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Vipera ammodytes ammodytes | ? | - |
? | |
bovine factor X + H2O | cleavage occurs at position Gln49-/-Cys50 in the light chain and at position Asp173-/-Leu174 in the heavy chain | Vipera ammodytes ammodytes | ? | - |
? | |
bovine oxidized insulin B-chain + H2O | the enzyme hydrolyzes at positions Gln4His5, His10Leu11 and Tyr16Leu17. No other peptide bond hydrolysis is detected after 30 min of incubation | Vipera ammodytes ammodytes | ? | - |
? | |
bovine prothrombin + H2O | - |
Vipera ammodytes ammodytes | ? | - |
? | |
Fibronectin + H2O | - |
Vipera ammodytes ammodytes | ? | - |
? | |
human fibrinogen alpha-chain + H2O | hydrolysis at multiple positions, including Ser220-/-Gln221, Lys413-/-Leu414, Glu422-/-Leu423 and Glu520-/-Phe521. Hydrolysis at lower rate (after 6 h) is detected at sites Asn109-/-Arg110, Arg23-/-Met240 and Arg491-/-His492 | Vipera ammodytes ammodytes | ? | - |
? | |
human fibrinogen beta-chain + H2O | the fibrinogen beta-chain is cleaved much slower than the alpha-chain | Vipera ammodytes ammodytes | ? | - |
? | |
additional information | the enzyme acts as a strong hemorrhagin in both rats and mice. Laminin is not cleaved even after 24 h treatment with ammodytagin. No cleavage of the human fibrinogen gamma-chain | Vipera ammodytes ammodytes | ? | - |
? | |
Nidogen + H2O | in Matrigel Growth Factor Reduced, nidogen is cleaved at positions Val422-/-Phe423 and Tyr352-/-Asn353 | Vipera ammodytes ammodytes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 50000 + 1 * 60000, SDS-PAGE under reducing conditions | Vipera ammodytes ammodytes |