Information on EC 3.4.24.B38 - ammodytagin

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The expected taxonomic range for this enzyme is: Vipera ammodytes ammodytes

EC NUMBER
COMMENTARY hide
3.4.24.B38
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
ammodytagin
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
The enzyme hydrolyzes bovine insulin B-chain at positions Gln4-/-His5, His10-/-Leu11 and Tyr16-/-Leu17. The enzyme possesses potent azocaseinolytic activity.
show the reaction diagram
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
azocasein + H2O
?
show the reaction diagram
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-
?
bovine factor X + H2O
?
show the reaction diagram
cleavage occurs at position Gln49-/-Cys50 in the light chain and at position Asp173-/-Leu174 in the heavy chain
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?
bovine oxidized insulin B-chain + H2O
?
show the reaction diagram
the enzyme hydrolyzes at positions Gln4–His5, His10–Leu11 and Tyr16–Leu17. No other peptide bond hydrolysis is detected after 30 min of incubation
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?
bovine prothrombin + H2O
?
show the reaction diagram
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?
Fibronectin + H2O
?
show the reaction diagram
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?
human fibrinogen alpha-chain + H2O
?
show the reaction diagram
hydrolysis at multiple positions, including Ser220-/-Gln221, Lys413-/-Leu414, Glu422-/-Leu423 and Glu520-/-Phe521. Hydrolysis at lower rate (after 6 h) is detected at sites Asn109-/-Arg110, Arg23-/-Met240 and Arg491-/-His492
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?
human fibrinogen beta-chain + H2O
?
show the reaction diagram
the fibrinogen beta-chain is cleaved much slower than the alpha-chain
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?
Nidogen + H2O
?
show the reaction diagram
in Matrigel Growth Factor Reduced, nidogen is cleaved at positions Val422-/-Phe423 and Tyr352-/-Asn353
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
5 mM, slightly enhances activity for 19.2%
Mg2+
5 mM, slightly enhances activity for 16.9%
Zn2+
Zn2+-dependent enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
1 mM, reduces activity to 2.8%
EDTA
5 mM, inhibits azocaseinolytic activity
L-cysteine
5 mM, reduces activity to 52%
Zn2+
5 mM, inhibits azocaseinolytic activity
additional information
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
1 * 50000 + 1 * 60000, SDS-PAGE under reducing conditions
60000
1 * 50000 + 1 * 60000, SDS-PAGE under reducing conditions
108000
MALDI mass spectrometry
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
1 * 50000 + 1 * 60000, SDS-PAGE under reducing conditions
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
total carbohydrate content is approximately 7.1%
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE