Literature summary for 3.4.24.69 extracted from

Elliott, M.; Favre-Guilmard, C.; Liu, S.M.; Maignel, J.; Masuyer, G.; Beard, M.; Boone, C.; Carre, D.; Kalinichev, M.; Lezmi, S.; Mir, I.; Nicoleau, C.; Palan, S.; Perier, C.; Raban, E.; Zhang, S.; Dong, M.; Stenmark, P.; Krupp, J.
Engineered botulinum neurotoxin B with improved binding to human receptors has enhanced efficacy in preclinical models (2019), Sci. Adv., 5, eaau7196 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Clostridium botulinum

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzyme E1191M/S1199Y in complex with synaptotagmin 1, sitting drop vapor diffusion method, using 1.1 M sodium malonate dibasic monohydrate, HEPES (pH 7.0), and 0.5% (v/v) Jeffamine ED-2003. Mutant enzyme E1191M/S1199Y in complex with synaptotagmin 2, sitting drop vapor diffusion method, using 0.2 M MgCl2, Tris (pH 7.0), and 10% (w/v) PEG 8000	Clostridium botulinum

Protein Variants

Protein Variants	Comment	Organism
E1191M/S1199Y	the mutation enhances binding to the human receptor synaptotagmin 2	Clostridium botulinum
E1191Q/S1199W	the mutation enhances binding to the human receptor synaptotagmin 2	Clostridium botulinum

Organism

Organism	UniProt	Comment	Textmining
Clostridium botulinum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
BuHP Sepharose column chromatography, Q Sepharose column chromatography, and phenyl Sepharose column chromatography	Clostridium botulinum

Synonyms

Synonyms	Comment	Organism
BoNT/B1	-	Clostridium botulinum
botulinum neurotoxin B	-	Clostridium botulinum

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Release 2023.1 (January 2023)