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Literature summary for 3.4.24.69 extracted from

  • Dhaliwal, H.K.; Thiruvanakarasu, N.; Kumar, R.; Patel, K.; Ambrin, G.; Cai, S.; Singh, B.R.
    High yield preparation of functionally active catalytic-translocation domain module of botulinum neurotoxin type A that exhibits uniquely different enzyme kinetics (2017), Protein J., 36, 489-501 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.69

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Clostridium botulinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0308
-
 SNAPtide 521 recombinant light chain-translocation domain, at pH 7.4 and 37°C Clostridium botulinum
0.10058
-
 SNAPtide 520 botulinum neurotoxin type A, at pH 7.4 and 37°C Clostridium botulinum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ dependent on Clostridium botulinum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 gel filtration Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
synaptosome-associated protein SNAP-25 + H2O Clostridium botulinum
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cobalt affinity column chromatography, trypsinization, and QAE Sephadex A-50 anion exchange column chromatography Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
SNAPtide 520 + H2O
-
 Clostridium botulinum ?
-
 ?
SNAPtide 521 + H2O
-
 Clostridium botulinum ?
-
 ?
synaptosome-associated protein SNAP-25 + H2O
-
 Clostridium botulinum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52200, light chain subunit of botulinum neurotoxin type A, SDS-PAGE Clostridium botulinum

Synonyms

Synonyms Comment Organism
BoNT/A
-
 Clostridium botulinum
botulinum neurotoxin type A
-
 Clostridium botulinum
LC/A light chain subunit of botulinum neurotoxin type A Clostridium botulinum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.052
-
 SNAPtide 521 recombinant light chain-translocation domain, at pH 7.4 and 37°C Clostridium botulinum
0.343
-
 SNAPtide 520 botulinum neurotoxin type A, at pH 7.4 and 37°C Clostridium botulinum

General Information

General Information Comment Organism
metabolism the light chain domain of BoNT/A specifically cleaves synaptosome associated protein of 25 kDa, resulting in the inhibition of neurotransmission at the synapse of peripheral neurons Clostridium botulinum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6
-
 SNAPtide 521 recombinant light chain-translocation domain, at pH 7.4 and 37°C Clostridium botulinum
3.3
-
 SNAPtide 520 botulinum neurotoxin type A, at pH 7.4 and 37°C Clostridium botulinum