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Literature summary for 3.4.24.69 extracted from

  • Tao, L.; Peng, L.; Berntsson, R.P.; Liu, S.M.; Park, S.; Yu, F.; Boone, C.; Palan, S.; Beard, M.; Chabrier, P.E.; Stenmark, P.; Krupp, J.; Dong, M.
    Engineered botulinum neurotoxin B with improved efficacy for targeting human receptors (2017), Nat. Commun., 8, 53 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.69

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clostridium botulinum

Protein Variants

Protein Variants Comment Organism
E1191C/S1199W the mutations enhance enzyme binding to human synaptotagmin II and human synaptotagmin I Clostridium botulinum
E1191M/S1199Y the mutant exhibits about 11fold higher efficacy in blocking neurotransmission than wild type botulinum neurotoxin B in neurons expressing human synaptotagmin II Clostridium botulinum
E1191V/S1199W the mutations enhance enzyme binding to human synaptotagmin II and human synaptotagmin I Clostridium botulinum
R370A/373F inactive Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
vesicle-associated membrane protein + H2O Clostridium botulinum
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and phenyl Sepharose column chromatography Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
vesicle-associated membrane protein + H2O
-
 Clostridium botulinum ?
-
 ?

Synonyms

Synonyms Comment Organism
BoNT/B
-
 Clostridium botulinum
botulinum neurotoxin B
-
 Clostridium botulinum