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Literature summary for 3.4.24.69 extracted from

  • Suzuki, T.; Kouguchi, H.; Watanabe, T.; Hasegawa, K.; Yoneyama, T.; Niwa, K.; Nishikawa, A.; Lee, J.C.; Oguma, K.; Ohyama, T.
    Effect of nicking the C-terminal region of the Clostridium botulinum serotype D neurotoxin heavy chain on its toxicity and molecular properties (2007), Protein J., 26, 173-181.
    View publication on PubMed
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 analytical gel filtration analysis of enzyme fragments from trypsin-derived nicks in the enzyme molecule, overview Clostridium botulinum
50000
-
 light chain of BoNT, analytical gel filtration Clostridium botulinum
100000
-
 heavy chain of BoNT, analytical gel filtration Clostridium botulinum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Clostridium botulinum toxicity in mice of full-length, single-nicked, and double-nicked enzyme forms, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium botulinum
-
 serotype D 4947
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification limited trypsin proteolysis of BoNT produces two nicks, the first nick yields a BoNT 50 kDa light chain disulfide linked to a 100 kDa heavy chain, a second nick arose in Hc C-terminal 10 kDa. The second nick occurrs in the putative binding domain of the BoNT molecule and induces alterations in its secondary structure, leading to a significant reduction of mouse toxicity in comparison with that of the fully activated singly nicked BoNT Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information toxicity in mice of full-length, single-nicked, and double-nicked enzyme forms, overview Clostridium botulinum ?
-
 ?

Synonyms

Synonyms Comment Organism
Clostridium botulinum serotype D neurotoxin
-
 Clostridium botulinum