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Literature summary for 3.4.24.63 extracted from
- Expression, purification and initial characterization of human meprin beta from Pichia pastoris (2015), Protein Expr. Purif., 116, 75-81 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene MEP1B, recombinant expression of C-terminally or N-terminally His6-tagged enzyme in Pichia pastoris strain X33 or SMD1168H. The N-terminal signal sequence is replaced by the alpha-leader of Saccharomyces, enabling efficient secretion of the mature enzyme, harboring either an N-terminal or C-terminal His-tag. The recombinant enzyme is N-glycosylated and secreted as a dimer | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Abz-MGWMDEIDK(Dnp)SG-OH | substrate inhibition | Homo sapiens |  |
| actinonin | - |
Homo sapiens | |
| NF 449 | 4,4',4'',4'''-[carbonyl- bis[imino-5,1,3-benzenetriyl-bis-(carbonylimino)]]tetrakis-(benzene-1,3-disulfonic acid) | Homo sapiens | |
| NNGH | - |
Homo sapiens | |
| PPNDS | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0218 | - |
Abz-MGWMDEIDK(Dnp)SG-OH | pH 8.0, 37°C, recombinant enzyme | Homo sapiens | |
| 0.0271 | - |
Abz-YVAEAPK(Dnp)G-OH | pH 8.0, 37°C, recombinant enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | - |
Homo sapiens | 9986 | - |
| membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | a single-zinc metalloendoprotease | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 133000 | - |
recombinant deglycosylated enzyme, gel filtration | Homo sapiens |
| 148000 | - |
recombinant glycosylated enzyme, gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q16820 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the recombinant enzyme expressed in Pichia pastoris is N-glycosylated. Deglycosylation by endoglycosidase H does not alter the kinetics of the enzyme significantly | Homo sapiens |
| proteolytic modification | pro-meprin beta is activated by trypsin (15:1 w/w) at room temperature in the presence of 10 mM CaCl2 for 10 min. Subsequently, trypsin is inactivated by the addition of 5 mM 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF) | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Pichia pastoris strain X33 or SMD1168H by nickel affinity chromatography, enzyme activation by trypsin, and hydrophobic interaction chromatography, followed by gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Abz-MGWMDEIDK(Dnp)SG-OH + H2O | - |
Homo sapiens | Abz-MGWM + DEIDK(Dnp)SG-OH | - |
? | |
| Abz-YVAEAPK(Dnp)G-OH + H2O | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| h-meprin beta | - |
Homo sapiens |
| meprin beta | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 35 | - |
Abz-YVAEAPK(Dnp)G-OH | pH 8.0, 37°C, recombinant enzyme | Homo sapiens | |
| 71.7 | - |
Abz-MGWMDEIDK(Dnp)SG-OH | pH 8.0, 37°C, recombinant enzyme | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant enzyme | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
NF 449 | pH 8.0, 37°C, recombinant enzyme | Homo sapiens | |
| 0.232 | - |
actinonin | pH 8.0, 37°C, recombinant enzyme | Homo sapiens | |
| 2.64 | - |
PPNDS | pH 8.0, 37°C, recombinant enzyme | Homo sapiens | |
| 8.58 | - |
NNGH | pH 8.0, 37°C, recombinant enzyme | Homo sapiens | |
| 52.9 | - |
Abz-MGWMDEIDK(Dnp)SG-OH | pH 8.0, 37°C, recombinant enzyme | Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | human meprin beta ia a single-zinc metalloendoprotease of the astacin family | Homo sapiens |
| physiological function | meprin beta is potentially involved in disorders such as fibrosis and Alzheimer's disease | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1291.5 | - |
Abz-YVAEAPK(Dnp)G-OH | pH 8.0, 37°C, recombinant enzyme | Homo sapiens | |
| 3289 | - |
Abz-MGWMDEIDK(Dnp)SG-OH | pH 8.0, 37°C, recombinant enzyme | Homo sapiens | |
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