Cloned (Comment) | Organism |
---|---|
gene MEP1B, recombinant expression of meprin beta in Pichia pastoris strain X-33 | Homo sapiens |
gene MEP1B, recombinant expression of N-terminally His6-tagged meprin beta Pichia pastoris strain X33 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-[[(3-carboxyphenyl)sulfonyl-[2-(hydroxyamino)-2-oxo-ethyl]amino]methyl]benzoic acid | hyperbolic noncompetitive/mixed-type inhibition; mixed type inhibition | Homo sapiens | |
3-[[[2-(hydroxyamino)-2-oxo-ethyl]-(4-methoxyphenyl)sulfonylamino]methyl]benzoic acid | hyperbolic noncompetitive/mixed-type inhibition; mixed type inhibition | Homo sapiens | |
actinonin | mixed type inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens | |
additional information | - |
additional information | enzyme kinetic data for meprin beta suggests hyperbolic v/S characteristics | Homo sapiens | |
0.074 | - |
KKGYVADAP-7-amido-4-methylcoumarin | pH 7.5, 30°C, recombinant enzyme | Homo sapiens | |
0.074 | - |
KKGYVADAP-7-amido-4-methylcoumarin | pH 7.4, 30°C, recombinant enzyme | Homo sapiens | |
0.184 | - |
KKGYVADAP-4-nitroanilide | pH 7.5, 30°C, recombinant enzyme | Homo sapiens | |
0.184 | - |
KKGYVADAP-4-nitroanilide | pH 7.4, 30°C, recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens | |
Zn2+ | required, a zinc-metalloprotease | Homo sapiens | |
Zn2+ | zinc-dependent metalloendoprotease | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | meprin beta preferentially cleaves substrates with acidic amino acids in P1'-position | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16820 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the recombinant His6-tagged enzyme is activated by cleavage through trypsin | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged meprin beta from Pichia pastoris strain X33 by nickel affinity chromatography, activation of the enzyme by trypsin, followed by hydrophobic interaction chromatography, and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
KKGYVADAP-4-nitroanilide + H2O | - |
Homo sapiens | KKGYVA + DAP-4-nitroanilide | - |
? | |
KKGYVADAP-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | KKGYVA + DAP-7-amido-4-methylcoumarin | - |
? | |
additional information | meprin beta preferentially cleaves substrates with acidic amino acids in P1'-position | Homo sapiens | ? | - |
? | |
additional information | the cleavage of a meprin beta substrate leads to generation of the prolyl tripeptidyl aminopeptidase (PtP, EC 3.4.14.12) substrate, and the activity of PtP results in release of a chromophore or fluorophore, coupled assay method evaluation, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MEP1B | - |
Homo sapiens |
meprin A subunit beta | UniProt | Homo sapiens |
meprin beta | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
KKGYVADAP-7-amido-4-methylcoumarin | pH 7.5, 30°C, recombinant enzyme | Homo sapiens | |
4 | - |
KKGYVADAP-7-amido-4-methylcoumarin | pH 7.4, 30°C, recombinant enzyme | Homo sapiens | |
20 | - |
KKGYVADAP-4-nitroanilide | pH 7.5, 30°C, recombinant enzyme | Homo sapiens | |
20 | - |
KKGYVADAP-4-nitroanilide | pH 7.4, 30°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
7.5 | - |
- |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Homo sapiens | |
0.00003 | - |
3-[[(3-carboxyphenyl)sulfonyl-[2-(hydroxyamino)-2-oxo-ethyl]amino]methyl]benzoic acid | pH 7.5, 30°C, recombinant enzyme | Homo sapiens | |
0.00003 | - |
3-[[[2-(hydroxyamino)-2-oxo-ethyl]-(4-methoxyphenyl)sulfonylamino]methyl]benzoic acid | pH 7.5, 30°C, recombinant enzyme | Homo sapiens | |
0.00003 | - |
3-[[(3-carboxyphenyl)sulfonyl-[2-(hydroxyamino)-2-oxo-ethyl]amino]methyl]benzoic acid | pH 7.4, 30°C, recombinant enzyme | Homo sapiens | |
0.00003 | - |
3-[[[2-(hydroxyamino)-2-oxo-ethyl]-(4-methoxyphenyl)sulfonylamino]methyl]benzoic acid | pH 7.4, 30°C, recombinant enzyme | Homo sapiens | |
0.0003 | - |
actinonin | pH 7.5, 30°C, recombinant enzyme | Homo sapiens | |
0.0003 | - |
actinonin | pH 7.4, 30°C, recombinant enzyme | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the astacin proteases meprin alpha and meprin beta are zinc-dependent metalloproteases of the metzincin superfamily | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
54.1 | - |
KKGYVADAP-7-amido-4-methylcoumarin | pH 7.5, 30°C, recombinant enzyme | Homo sapiens | |
54.1 | - |
KKGYVADAP-7-amido-4-methylcoumarin | pH 7.4, 30°C, recombinant enzyme | Homo sapiens | |
108.7 | - |
KKGYVADAP-4-nitroanilide | pH 7.5, 30°C, recombinant enzyme | Homo sapiens | |
108.7 | - |
KKGYVADAP-4-nitroanilide | pH 7.4, 30°C, recombinant enzyme | Homo sapiens |