Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Hathewaya histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EGTA | Ca2+ chelation by EGTA induces interdomain conformational changes with an increase in the percent polydispersity as the Ca2+ is chelated, suggesting an increase in protein flexibility. The thermostability is decreased by Ca2+ chelation | Hathewaya histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | interdomain flexibility and the domain arrangement of full-length collagenase H are reversibly regulated by Ca2+ | Hathewaya histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
gel filtration | Hathewaya histolytica |
114000 | - |
1 * 114000, calculated | Hathewaya histolytica |
119000 | - |
gel filtration, presence of EGTA | Hathewaya histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hathewaya histolytica | Q46085 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azokoll + H2O | - |
Hathewaya histolytica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 114000, calculated | Hathewaya histolytica |
Synonyms | Comment | Organism |
---|---|---|
ColH | - |
Hathewaya histolytica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
49 | - |
melting point, presence of EGTA. Addition of excess Ca2+ restores melting point to 54°C | Hathewaya histolytica |
54 | - |
melting point | Hathewaya histolytica |