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Literature summary for 3.4.23.B11 extracted from

  • Pfrepper, K.I.; Lochelt, M.; Schnolzer, M.; Flugel, R.M.
    Expression and molecular characterization of an enzymatically active recombinant human spumaretrovirus protease (1997), Biochem. Biophys. Res. Commun., 237, 548-553.
    View publication on PubMed
Search for more literature for 3.4.23.B11

Cloned(Commentary)

Cloned (Comment) Organism
the enzyme is cloned into a modified thioredoxin fusion vector that carries a His-tag in the centrally located surface loop of the Escherichia coli trxA protein, bacterially expressed as a soluble fusion protein Human spumaretrovirus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24500
-
 x * 24500, recombinant enzyme, SDS-PAGE Human spumaretrovirus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pr74 + H2O Human spumaretrovirus the cleavage site that leads to the formation of the HFV gag proteins p70 and p3 is located 27 amino acid residues upstream of the carboxy terminus of the p74 gag precursor ?
-
 ?

Organism

Organism UniProt Comment Textmining
Human spumaretrovirus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Human spumaretrovirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pr74 + H2O the cleavage site that leads to the formation of the HFV gag proteins p70 and p3 is located 27 amino acid residues upstream of the carboxy terminus of the p74 gag precursor Human spumaretrovirus ?
-
 ?
RAVNTVTQ + H2O
-
 Human spumaretrovirus RAVN + TVTQ
-
 ?

Subunits

Subunits Comment Organism
? x * 24500, recombinant enzyme, SDS-PAGE Human spumaretrovirus