Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.23.B11 extracted from

  • Fenyofalvi, G.; Bagossi, P.; Copeland, T.D.; Oroszlan, S.; Boross, P.; Tozser, J.
    Expression and characterization of human foamy virus proteinase (1999), FEBS Lett., 462, 397-401.
    View publication on PubMed
Search for more literature for 3.4.23.B11

Cloned(Commentary)

Cloned (Comment) Organism
expression of a 141 residue long part of the HFV as a fusion protein with maltose binding protein in Escherichia coli Human spumaretrovirus

General Stability

General Stability Organism
50% loss of activity at 0.75 M urea Human spumaretrovirus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
 VPTINNVHTS pH 6.6, 37°C Human spumaretrovirus
0.15
-
 SRAVYTITPE pH 6.6, 37°C Human spumaretrovirus
0.56
-
 SRAVNTVTOS pH 6.6, 37°C Human spumaretrovirus
0.91
-
 GSYVVNCNTKK pH 6.6, 37°C Human spumaretrovirus
1
-
 IHLIAAVKAV pH 6.6, 37°C Human spumaretrovirus

Organism

Organism UniProt Comment Textmining
Human spumaretrovirus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GSYVVNCNTKK + H2O
-
 Human spumaretrovirus GSYVVN + CNTKK
-
 ?
IHLIAAVKAV + H2O
-
 Human spumaretrovirus IHLIA + AVKAV
-
 ?
SRAVNTVTOS + H2O oligopeptide representing the Gag(nucleocapsi)-cleavage site in human foamy virus Human spumaretrovirus SRAVN + TVTOS
-
 ?
SRAVYTITPE + H2O
-
 Human spumaretrovirus SRAVY + TITPE
-
 ?
VPTINNVHTS + H2O
-
 Human spumaretrovirus VPTIN + NVHTS
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0002
-
 VPTINNVHTS pH 6.6, 37°C Human spumaretrovirus
0.001
-
 SRAVYTITPE pH 6.6, 37°C Human spumaretrovirus
0.002
-
 IHLIAAVKAV pH 6.6, 37°C Human spumaretrovirus
0.003
-
 GSYVVNCNTKK pH 6.6, 37°C Human spumaretrovirus
0.006
-
 SRAVNTVTOS pH 6.6, 37°C Human spumaretrovirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
-
 Human spumaretrovirus

pH Range

pH Minimum pH Maximum Comment Organism
5.8 7.6 pH 5.8: about 65% of maximal activity, pH 7.6: about 45% of maximal activity Human spumaretrovirus