Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli inclusion bodies, expression in insect cells | Sulfolobus acidocaldarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2-epoxy-3-(4-nitrophenoxy)propane | inactivation | Sulfolobus acidocaldarius | |
acetamide | - |
Sulfolobus acidocaldarius | |
Diazoacetyl-DL-norleucine methyl ester | i.e. DAN, inactivation | Sulfolobus acidocaldarius | |
Mercuric acetate | - |
Sulfolobus acidocaldarius | |
additional information | no inhibition by iodoacetate and 4-chloromercuribenzoate | Sulfolobus acidocaldarius | |
pepstatin A | competitive inhibition | Sulfolobus acidocaldarius | |
phenylalaninamide | - |
Sulfolobus acidocaldarius | |
Urea | - |
Sulfolobus acidocaldarius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | attached to the cell suface | Sulfolobus acidocaldarius | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32651 | - |
x * 32651, amino acid sequence calculation, x * 46000-51000, glycosylated enzyme | Sulfolobus acidocaldarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hemoglobin + H2O | Sulfolobus acidocaldarius | - |
? | - |
? | |
additional information | Sulfolobus acidocaldarius | the enzyme shows a broad protein substrate specificity, it is involved in supply of nutrients from protein substrates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | - |
originally isolated from hot springs of the Yellowstone Park | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme has 11 potential glycosylation sites, e.g. at Asn24 and Asn28 | Sulfolobus acidocaldarius |
Purification (Comment) | Organism |
---|---|
native enzyme from cell culture by solubilization with detergents, ultrafiltration and five chromatographic steps to homogeneity | Sulfolobus acidocaldarius |
Renatured (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli inclusion bodies by treatment with 8 M urea, incubation in acid solution, and gel filtration | Sulfolobus acidocaldarius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimal cell cultivation at pH 2.0 and 70°C | Sulfolobus acidocaldarius | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FVNQHLCGSHLVEALYLVCGERGFFYTPKA + H2O | substrate insulin B chain | Sulfolobus acidocaldarius | FVNQHLCGSHL + VEAL + YLVCGERGF + L-Phe + YTPKA | - |
? | |
Hemoglobin + H2O | - |
Sulfolobus acidocaldarius | ? | - |
? | |
Lys-Pro-Ala-Glu-Phe-Nph-Ala-Leu + H2O | synthetic chromogenic substrate | Sulfolobus acidocaldarius | Lys-Pro-Ala-Glu-Phe + Nph-Ala-Leu | - |
? | |
additional information | the enzyme shows a broad protein substrate specificity, it is involved in supply of nutrients from protein substrates | Sulfolobus acidocaldarius | ? | - |
? | |
additional information | the enzyme shows a broad protein substrate specificity | Sulfolobus acidocaldarius | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32651, amino acid sequence calculation, x * 46000-51000, glycosylated enzyme | Sulfolobus acidocaldarius |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the A5 peptidase family | Sulfolobus acidocaldarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | substrate hemoglobin | Sulfolobus acidocaldarius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
100 | activity range, substrate hemoglobin, slow activity increase between 26°C and 65°C, rapid increase above 65°C | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
- |
Sulfolobus acidocaldarius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
- |
12 | activity range, highest activity at pH 1.0- pH 5.0, substrate hemoglobin | Sulfolobus acidocaldarius |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
pepstatin A | 56°C | Sulfolobus acidocaldarius | |
0.0002 | - |
pepstatin A | 76°C | Sulfolobus acidocaldarius | |
0.00049 | - |
pepstatin A | 37°C | Sulfolobus acidocaldarius | |
10 | - |
phenylalaninamide | - |
Sulfolobus acidocaldarius | |
400 | - |
acetamide | - |
Sulfolobus acidocaldarius | |
500 | - |
Urea | - |
Sulfolobus acidocaldarius |