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Literature summary for 3.4.23.34 extracted from
- Isolation and characterization of human gastric procathepsin E and cathepsin E (1995), Adv. Exp. Med. Biol., 362, 201-210.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | 1,10-phenanthroline, diprotin A, antipain, amastatin, L-trans-epoxysuccinyl-leucylamido-(4-guanidino)-butane (i.e. E-64, at pH 7.4); inhibition by PMSF, diisopropyl fluorophosphate, leupeptin | Homo sapiens | |
| pepstatin | as substrate, incubation at pH 7.4 restores; at pH 3 and 5.5, not at pH 7.4; oxidized insulin B-chain | Homo sapiens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE, reducing conditions | Homo sapiens |
| 40000 | - |
2 * 40000, procathepsin E, SDS-PAGE, reducing conditions | Homo sapiens |
| 76000 | - |
human, SDS-PAGE, non-reducing conditions | Homo sapiens |
| 80000 | - |
human, procathepsin E, SDS-PAGE, non-reducing conditions | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | site of carbohydrate attachment is Asn73 | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| as procathepsin E with following activation by lowering the pH-value of the solution to 4 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gastric mucosa | - |
Homo sapiens | - |
| stomach | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, at pH 8, several months, with some autodigestion | Homo sapiens |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE, reducing conditions | Homo sapiens |
| dimer | 2 * 40000, procathepsin E, SDS-PAGE, reducing conditions | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 5.5 | 6 h at 37°C, unstable | Homo sapiens |
| 6 | 7.4 | 6 h at 37°C, rather stable | Homo sapiens |
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