Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Human immunodeficiency virus 1 |
Crystallization (Comment) | Organism |
---|---|
PRS17-D25N alone and in complex with substrate analogues RVLrFEANle and Ace-TINlerNleQR , hanging drop vapor diffusion method, using 1.95 M sodium chloride and 0.1 M bis-Tris pH 7.5 (inhibitor-free), 2.1 M sodium chloride and 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid at pH 7.6 (with RVLrFEANle) or 29.5% (w/v) poly(ethylene glycol) 4000, 0.2 M ammonium acetate, and 0.1 M sodium acetate buffer at pH 4.6 (with Ace-TINlerNleQR) | Human immunodeficiency virus 1 |
Protein Variants | Comment | Organism |
---|---|---|
PRS17 | the highly drug-resistant mutant enzyme shows enhanced binding to substrate analogues RVLrFEANle and Ace-TINlerNleQR | Human immunodeficiency virus 1 |
PRS17-D25N | inactive | Human immunodeficiency virus 1 |
PRV82A | the highly drug-resistant mutant enzyme shows enhanced binding to substrate analogues RVLrFEANle and Ace-TINlerNleQR | Human immunodeficiency virus 1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ace-TINlerNleQR | - |
Human immunodeficiency virus 1 | |
amprenavir | - |
Human immunodeficiency virus 1 | |
darunavir | - |
Human immunodeficiency virus 1 | |
indinavir | - |
Human immunodeficiency virus 1 | |
RVLrFEANle | - |
Human immunodeficiency virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | Q5RZ08 | - |
- |
Purification (Comment) | Organism |
---|---|
Source 15RPC column chromatography and Superdex 75 gel filtration | Human immunodeficiency virus 1 |
Synonyms | Comment | Organism |
---|---|---|
HIV-1 protease | - |
Human immunodeficiency virus 1 |
human immunodeficiency virus protease | - |
Human immunodeficiency virus 1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000022 | - |
Ace-TINlerNleQR | mutant enzyme PRS17, at pH 5.6 and 37°C | Human immunodeficiency virus 1 | |
0.000024 | - |
Ace-TINlerNleQR | mutant enzyme PRV82A, at pH 5.6 and 37°C | Human immunodeficiency virus 1 | |
0.000075 | - |
Ace-TINlerNleQR | wild type enzyme, at pH 5.6 and 37°C | Human immunodeficiency virus 1 | |
0.000514 | - |
RVLrFEANle | mutant enzyme PRS17, at pH 5.6 and 37°C | Human immunodeficiency virus 1 | |
0.00053 | - |
RVLrFEANle | mutant enzyme PRV82A, at pH 5.6 and 37°C | Human immunodeficiency virus 1 | |
0.00217 | - |
RVLrFEANle | wild type enzyme, at pH 5.6 and 37°C | Human immunodeficiency virus 1 |