Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Shewanella denitrificans |
Protein Variants | Comment | Organism |
---|---|---|
D37A | inactive | Shewanella denitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by Pefabloc, EDTA, E-64, amstatin, and dithiohtreitol | Shewanella denitrificans | |
pepstatin | complete inhibition at 0.001 mM | Shewanella denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00867 | - |
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | at pH 4.0 and 37°C | Shewanella denitrificans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Shewanella denitrificans | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella denitrificans | Q12R32 | - |
- |
Purification (Comment) | Organism |
---|---|
Co2+-carged His-Trap HP column chromatography, pepstatin-agarose affinity chromatography, and Mono Q column chromatography | Shewanella denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) + H2O | - |
Shewanella denitrificans | ((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe + Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | - |
? | |
additional information | no activity with ((7-methoxycoumarin-4-yl)acetyl)-Lys-Leu-His-Pro-Glu-Val-Leu-Phe-Val-Leu-Glu-Lys-(2,4-dinitrophenyl), Arg-Glu-(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys-(DABCYL)-Arg, ((7-methoxycoumarin-4-yl)acetyl)-Lys-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys-(2,4-dinitrophenyl), or ((7-methoxycoumarin-4-yl)acetyl)-Lys-Ala-Leu-Ile-Pro-Ser-Tyr-Lys-Trp-Ser-Lys-(2,4-dinitrophenyl) | Shewanella denitrificans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Shewanella denitrificans |
? | x * 49025, calculated from amino acid sequence | Shewanella denitrificans |
Synonyms | Comment | Organism |
---|---|---|
shewasin D | - |
Shewanella denitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | - |
Shewanella denitrificans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 40 | maximum enzyme activity is detected between 30°C and 37°C. Above this temperature the activity abruptly decreases, with no detectable activity remaining at 50°C. At lower temperatures the activity decreases gradually, with the enzyme retaining 30% activity at 10°C | Shewanella denitrificans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.51 | - |
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | at pH 4.0 and 37°C | Shewanella denitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4 | - |
Shewanella denitrificans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5 | maximum activity towards this substrate at pH values between pH 3.5 and 4.0, with a drastic decrease in activity of 50% and 70% at pH values 3.0 and 5.0, respectively | Shewanella denitrificans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
400 | - |
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl) | at pH 4.0 and 37°C | Shewanella denitrificans |