Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by EDTA, E-64, leupeptin, 4-(2-aminoethyl)benzenesulfonyl fluoride or aprotinin | Oncorhynchus mykiss | |
pepstatin A | - |
Oncorhynchus mykiss |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000079 | - |
Acid-denatured hemoglobin | isoform P-III, at pH 2.0 and 37°C | Oncorhynchus mykiss | |
0.000013 | - |
Acid-denatured hemoglobin | isoform P-II, at pH 2.0 and 37°C | Oncorhynchus mykiss | |
0.000028 | - |
Acid-denatured hemoglobin | isoform P-I, at pH 2.0 and 37°C | Oncorhynchus mykiss |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oncorhynchus mykiss | - |
rainbow trout | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE Sepharose column chromatography and Superdex G75 gel filtration | Oncorhynchus mykiss |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | - |
Oncorhynchus mykiss | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Acid-denatured hemoglobin + H2O | - |
Oncorhynchus mykiss | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, isoform P-II, SDS-PAGE | Oncorhynchus mykiss |
? | x * 37000, isoform P-Ia, SDS-PAGE | Oncorhynchus mykiss |
? | x * 37000, isoform P-Ib, SDS-PAGE | Oncorhynchus mykiss |
? | x * 37000, isoform P-III, SDS-PAGE | Oncorhynchus mykiss |
Synonyms | Comment | Organism |
---|---|---|
P-Ia | isoform | Oncorhynchus mykiss |
P-Ib | isoform | Oncorhynchus mykiss |
P-II | isoform | Oncorhynchus mykiss |
P-III | isoform | Oncorhynchus mykiss |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
isoforms P-II and P-III | Oncorhynchus mykiss |
40 | - |
isoform P-I | Oncorhynchus mykiss |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.43 | - |
Acid-denatured hemoglobin | isoform P-I, at pH 2.0 and 37°C | Oncorhynchus mykiss | |
8.37 | - |
Acid-denatured hemoglobin | isoform P-II, at pH 2.0 and 37°C | Oncorhynchus mykiss | |
19.1 | - |
Acid-denatured hemoglobin | isoform P-III, at pH 2.0 and 37°C | Oncorhynchus mykiss |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
isoforms P-II and P-III | Oncorhynchus mykiss |
3 | - |
isoform P-I | Oncorhynchus mykiss |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1 | 6 | trout pepsins exhibit the highest enzyme activity at pH 3.0 (isoform P-I) and 2.5 (isoforms P-II and P-III); P-I shows a broad optimum in the pH range from 1.5 to 4.0, while the optima for P-II and III are restricted to the pH range from 2.0 to 3.0.at pH 4.0. Isoform P-I retains 94% activity, while isoforms P-II 72 and P-III have 65% activity. All three isoforms show residual activity of about 20% at pH 6.0 | Oncorhynchus mykiss |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Oncorhynchus mykiss | isoform P-IIIa, isoelectric focusing | - |
3.7 |
Oncorhynchus mykiss | isoform P-IIIb, isoelectric focusing | - |
3.8 |
Oncorhynchus mykiss | isoform P-Ia, isoelectric focusing | - |
4 |
Oncorhynchus mykiss | isoform P-Ib, isoelectric focusing | - |
4 |
Oncorhynchus mykiss | isoform P-IIIc, isoelectric focusing | - |
4 |
Oncorhynchus mykiss | isoform P-IIId, isoelectric focusing | - |
4.2 |
Oncorhynchus mykiss | isoform P-II, isoelectric focusing | - |
5.9 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
190000 | - |
Acid-denatured hemoglobin | isoform P-I, at pH 2.0 and 37°C | Oncorhynchus mykiss | |
640000 | - |
Acid-denatured hemoglobin | isoform P-II, at pH 2.0 and 37°C | Oncorhynchus mykiss | |
2400000 | - |
Acid-denatured hemoglobin | isoform P-III, at pH 2.0 and 37°C | Oncorhynchus mykiss |