Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the pepsinogen isoform converts into the active form pepsin under pH 2.0 | Lateolabrax japonicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin A | complete inhibition at a molar ratio of 1:20 (pepstatin A:pepsin) | Lateolabrax japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
hemoglobin | isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C | Lateolabrax japonicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, pepsin isoform P1, SDS-PAGE | Lateolabrax japonicus |
35000 | - |
x * 35000, pepsinogen isoform PG1, SDS-PAGE | Lateolabrax japonicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Lateolabrax japonicus | the cleavage site of isoform PG1 to produce pepsin is clearly at the 41-42 bond of Phe-Ala | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lateolabrax japonicus | E2IKQ9 | pepsinogen isoform A1; Japanese seabass | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sephacel column chromatography, and Sephacryl S-200 gel filtration | Lateolabrax japonicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | - |
Lateolabrax japonicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the specific activity of the crude extract and 7.9fold purified enzyme are 4.0 and 31.5 units/mg. One unit of pepsin activity is defined as an increase of absorbance of 1.0 at 280 nm during an incubation time of 30 min at pH 3.0 and 37°C | Lateolabrax japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hemoglobin + H2O | - |
Lateolabrax japonicus | ? | - |
? | |
additional information | the cleavage site of isoform PG1 to produce pepsin is clearly at the 41-42 bond of Phe-Ala | Lateolabrax japonicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, pepsin isoform P1, SDS-PAGE | Lateolabrax japonicus |
? | x * 35000, pepsinogen isoform PG1, SDS-PAGE | Lateolabrax japonicus |
Synonyms | Comment | Organism |
---|---|---|
pepsin 1 | pepsin A isoform 1 | Lateolabrax japonicus |
PG1 | pepsinogen isoform | Lateolabrax japonicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Lateolabrax japonicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
at 50°C and pH 7.0, the half-life of isoform P1 is 35 min | Lateolabrax japonicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.34 | - |
hemoglobin | isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C | Lateolabrax japonicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
- |
Lateolabrax japonicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lateolabrax japonicus | pepsinogen isoform PG1, isoelectric focusing | - |
5.3 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
130 | - |
hemoglobin | isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C | Lateolabrax japonicus |