Cloned (Comment) | Organism |
---|---|
overexpression of CPB2.8 as an inactive pro-form lacking the characteristic C-terminal extension, CPB2.8DELTACTE in Escherichia coli. Pro-region processing is initiated during protein refolding and proceeds through several intermediate stages. Maximum enzyme activity after removal of the entire pro-region. This is facilitated by acidification | Leishmania mexicana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | - |
Leishmania mexicana | |
E-64 | - |
Leishmania mexicana | |
leupeptin | effective inhibitor of the recombinant mature CPB2.8DELTACTE | Leishmania mexicana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
N-benzoyl-Pro-Phe-Arg-p-nitroanilide | pH 6.0, 37°C | Leishmania mexicana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inclusion body | recombinant enzyme from Escherichia coli | Leishmania mexicana | 16234 | - |
inclusion body | recombinant enzyme expressed in Escherichia coli | Leishmania mexicana | 16234 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
x * 26000, activated enzyme, SDS-PAGE | Leishmania mexicana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania mexicana | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | overexpression of CPB2.8 as an inactive pro-form lacking the characteristic C-terminal extension, CPB2.8DELTACTE in Escherichia coli. Pro-region processing is initiated during protein refolding and proceeds through several intermediate stages. Maximum enzyme activity after removal of the entire pro-region. The activated enzyme has a MW of 26000 Da, the inactive pro-form of the enzyme has a MW of 38000 Da as determined by SDS-PAGE | Leishmania mexicana |
Purification (Comment) | Organism |
---|---|
- |
Leishmania mexicana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.16 | - |
- |
Leishmania mexicana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzoyl-Pro-Phe-Arg-p-nitroanilide + H2O | - |
Leishmania mexicana | N-benzoyl-Pro-Phe-Arg + p-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, activated enzyme, SDS-PAGE | Leishmania mexicana |
More | the inactive pro-form of the enzyme has a MW of 38000 Da as determined by SDS-PAGE | Leishmania mexicana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.8 | - |
N-benzoyl-Pro-Phe-Arg-p-nitroanilide | pH 6.0, 37°C | Leishmania mexicana |