Information on EC 3.4.22.B5 - CPB protease

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.22.B5
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
CPB protease
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
good cleavage of the Arg-Phe bond. The best substrate ortho-aminobenzoyl-KLR-/-FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) is also well hydrolyzed by cathepsin L, however the best inhibitor of the parasite enzyme ortho-aminobenzoyl-KLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) has low affinity to cathepsin L
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
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hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY hide
357959-79-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CT-IOC-067
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Manually annotated by BRENDA team
CT-IOC-067
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Manually annotated by BRENDA team
strain MHOM/BR/75 Josefa
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain MRHO/IR/75/ER
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Manually annotated by BRENDA team
strain MNYC/BZ/M379
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Manually annotated by BRENDA team
strain MYNC/BZ/62/M379
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Manually annotated by BRENDA team
isolate 9T
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
additional information
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recombinant enzyme mutant CPB2.8DEKTACTE structure homology modeling using 1.75 A resolution crystal structure of cruzain complexed with the irreversible fluoromethyl ketone inhibitor Mor-Leu-Hpq was used as a template, PDB ID 1EWP, overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-aminobenzoyl-ELRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-ELR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-FLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-FLR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-FRAK(Dnp)-NH2 + H2O
?
show the reaction diagram
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2-aminobenzoyl-FRAK(Dnp)-OH + H2O
?
show the reaction diagram
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2-aminobenzoyl-KERSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KER + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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-
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2-aminobenzoyl-KFRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KFR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KGRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KGR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLCSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLC + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLESSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLE + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLFSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLF + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLHSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLH + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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?
2-aminobenzoyl-KLLSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLL + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLNSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLN + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLRESKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLR + ESKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLRFSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLR + FSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLRGSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLR + GSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLRNSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLR + NSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLRRSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLR + RSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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?
2-aminobenzoyl-KLRSEKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLR + SEKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLRSFKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLR + SFKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLRSGKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLR + SGKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLRSLKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLR + SLKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLRSPKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLR + SPKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLRSRKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLR + SRKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KLR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KNRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KNR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-KRRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-KRR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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2-aminobenzoyl-LLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-LLR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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?
2-aminobenzoyl-NLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide) + H2O
2-aminobenzoyl-NLR + SSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
show the reaction diagram
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Abz-ALRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
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Abz-ARF(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
?
show the reaction diagram
Abz-ARFK-OH + H2O
?
show the reaction diagram
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Abz-FR(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
?
show the reaction diagram
Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
?
show the reaction diagram
Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys-amide + H2O
?
show the reaction diagram
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Abz-FRAK-NH2 + H2O
?
show the reaction diagram
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Abz-FRAK-OH + H2O
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show the reaction diagram
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Abz-FRF(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
?
show the reaction diagram
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Abz-FRFK-OH + H2O
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show the reaction diagram
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Abz-FRKP-OH + H2O
?
show the reaction diagram
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Abz-FRKW-OH + H2O
?
show the reaction diagram
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Abz-KLGFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
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Abz-KLRFSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
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?
Abz-peptidyl-Q-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
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Abz-RRF(2,4-dinitrophenyl)-epsilon-NH2-Lys + H2O
?
show the reaction diagram
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Abz-RRFK-OH + H2O
?
show the reaction diagram
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Ac-Phe-Arg-7-amido-4-methylcoumarin + H2O
Ac-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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50 microM, 13 nanoM cpB, 45 min, room temperature, pH 5.5
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?
albumin
?
show the reaction diagram
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37C, 48 h, pH 5.0
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benzoyl-(3-pyridylalanine)-R-4-methylcoumarin-7-amide + H2O
benzoyl-(3-pyridylalanine)-R + 7-amino-4-methylcoumarin
show the reaction diagram
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benzoyl-(4-aminomethyl-N-isopropyl-phenylalanine)-R-4-methylcoumarin-7-amide + H2O
benzoyl-(4-aminomethyl-N-isopropyl-phenylalanine) + 7-amino-4-methylcoumarin
show the reaction diagram
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benzoyl-(4-aminomethyl-phenylalanine)-R-4-methylcoumarin-7-amide + H2O
benzoyl-(4-aminomethyl-phenylalanine)-R + 7-amino-4-methylcoumarin
show the reaction diagram
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?
benzoyl-(4-guanidine-phenylalanine)-R-4-methylcoumarin-7-amide + H2O
benzoyl-(4-guanidine-phenylalanine)-R + 7-amino-4-methylcoumarin
show the reaction diagram
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?
benzoyl-FR-4-methylcoumarin-7-amide + H2O
benzoyl-FR + 7-amino-4-methylcoumarin
show the reaction diagram
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?
benzoyl-HR-4-methylcoumarin-7-amide + H2O
benzoyl-HR + 7-amino-4-methylcoumarin
show the reaction diagram
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benzoyl-RR-4-methylcoumarin-7-amide + H2O
benzoyl-RR + 7-amino-4-methylcoumarin
show the reaction diagram
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?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
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benzyloxycarbonyl-Phe-Arg-p-nitroanilide + H2O
benzyloxycarbonyl-Phe-Arg + p-nitroaniline
show the reaction diagram
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?
casein
?
show the reaction diagram
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37C, 48 h, pH 5.0
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gelatin
?
show the reaction diagram
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37C, 48 h, pH 5.0
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Gelatin + H2O
?
show the reaction diagram
GST-IkappaBalpha + H2O
?
show the reaction diagram
haemoglobin
?
show the reaction diagram
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37C, 48 h, pH 5.0
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?
mucin
?
show the reaction diagram
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37C, 48 h, pH 5.0
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?
N-benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O
N-benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
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isoenzyme CPB1, CPB2.8 and CPB18 show almost proficient hydrolyzing activity
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?
N-benzoyl-Pro-Phe-Arg-p-nitroanilide + H2O
N-benzoyl-Pro-Phe-Arg + p-nitroaniline
show the reaction diagram
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?
N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
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isoenzyme CPB18 has significant higher activity than isoenzymes CPB1 or CPB2.8
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non-immune immunoglobulin
?
show the reaction diagram
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37C, 48 h, pH 5.0
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NPHAQFGITKFFDK-NH2 + H2O
NPHAQFGIT + KFFDK-NH2
show the reaction diagram
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o-aminobenzoyl-ALRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-ALR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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o-aminobenzoyl-ANHFLCTQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-ANHFL + CTQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-HLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-HLR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KARFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KAR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KFRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KFR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLC(SBzl)FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLC(SBzl) + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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o-aminobenzoyl-KLCFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLC + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLDFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLD + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLEFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLE + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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o-aminobenzoyl-KLFNPKFQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLF + NPKFQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLHFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLH + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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o-aminobenzoyl-KLKFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLK + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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o-aminobenzoyl-KLRASKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLR + ASKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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o-aminobenzoyl-KLRFAKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLR + FAKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLRFFKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLR + FFKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLRFPKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLR + FPKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLRFRKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLR + FRKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLRFSAQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLR + FSAQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLRFSPQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLR + FSPQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLRFSRQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLR + FSRQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLRLSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLR + LSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KLRRSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLR + RSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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-
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?
o-aminobenzoyl-KLSKYLSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLS + KYLSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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slow hydrolysis at D-/-K and L-/-S
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o-aminobenzoyl-KLT(OBzl)FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KLT(OBzl) + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KPRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KPLR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-KRRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-KRR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
o-aminobenzoyl-LLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-LLR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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-
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?
o-aminobenzoyl-RLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
o-aminobenzoyl-RLR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
show the reaction diagram
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?
pGlu-Phe-Leu-p-nitroanilide + H2O
pGlu-Phe-Leu + p-nitroaniline
show the reaction diagram
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?
RYLNGAAYFAAAKR-NH2 + H2O
?
show the reaction diagram
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hydrolysis at RYLNGAAYF-/-A-/-AAKR-NH2
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salivary gland extract
?
show the reaction diagram
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37C, 48 h, pH 5.0, salivary gland extract from Oncopeltus fasciatus
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YFAAAKRHAAQHYR-NH2 + H2O
YFA + AAKRHAAQHYR-NH2
show the reaction diagram
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Z-FR-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2(1H)-pyridinethionato-kappaS2)[2,6-bis[(mercapto-KappaS)methyl]pyridine-kappaN1] oxorhenium(V)
-
-
(methanethiolato)[2,2'-(thio-kappaS)bis[ethanethiolato-kappaS]] oxorhenium (V)
-
-
(p-methoxyphenylthiolato-S)[2,6-bis[(mercapto-kappaS)methyl]pyridine-kappaN] oxorhenium(V)
-
-
2-aminobenzoyl-KLRGSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
-
2-phenyl-1H-indole-3-carbaldehyde thiosemicarbazone
-
a thiosemicarbazon
3-[(1H-benzimidazol-2-yl)-2H-1-benzopyran-2-ylidene]-2-carbaldehyde semicarbazone
-
a semicarbazone
4-(cyclohexylamino)-6-[(2-methoxy-3-pyridinyl)amino]-1,3,5-triazine-2-carbonitrile
-
a nitrile
4-methyl N-[(S)-1-oxo-1-[(S)-1-oxo-4-phenyl-1-(5-phenylisoxazol-3-yl)butan-2-ylamino]-3-phenylpropan-2-yl]piperazine-1-carboxamide
-
-
5-(4-methoxy-phenyl)-2-furanyl-2-carbaldehyde thiosemicarbazone
-
a thiosemicarbazon
aceto[2,6-bis[(butylthio-jS)methyl]phenyl-kappaC]-,(SP-4-3)-palladium(II)
-
-
benzoyl-(4-aminomethyl-cyclohexyl-alanine)-R-4-methylcoumarin-7-amide
-
-
cathepsin inhibitor IV
-
-
-
chloro[2,2'-(thio-kappaS)bis[ethanethiolato-kappaS]] oxorhenium(V)
-
-
Cystatin
-
1 microM, 37C, 48 h, pH 5.0
-
diaceto[2-[(2-pyridinyl-kappaN)methyl]-phenyl-kappaC]Au(III)-,(SP-4-3)
-
-
EAEFAARYLNGAAYF-NH2
-
-
FAARYLNGA
-
-
FAARYLNGA-NH2
-
-
FAARYLNGAQ-[N-(2,4-dinitrophenyl)]-ethylenediamine
-
-
ITKFFDLSEAEFAA-NH2
-
-
leupeptin
N-((S)-1-[(S)-1-[1-(benzyloxymethyl)-1H-1,2,3-triazol-4-yl]-1-oxo-3-phenylpropan-2-ylamino]-1-oxo-3-phenylpropan-2-yl)morpholine-4-carboxamide
-
-
N-[(2S)-1-[(2S)-1-hydroxy-3-phenyl-1-(5-phenyl-1,2,4-oxadiazol-3-yl)propan-2-ylamino]-1-oxo-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
-
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(1-phenyl-1H-1,2,3-triazol-4-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
-
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(3-phenylisoxazol-5-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
-
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(5-phenylisoxazol-3-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
-
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(5-phenyloxazol-2-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
-
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(5-phenylthiazol-2-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
-
N-[(S)-1-[(d)-1-(5-benzylisoxazol-3-yl)-1-oxo-4-phenylbutan-2-ylamino]-1-oxo-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
-
N-[(S)-1-[(S)-1-(benzo[d]oxazol-2-yl)-1-oxo-3-phenylpropan-2-ylamino]-1-oxo-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
-
N-[(S)-1-[(S)-1-(benzo[d]thiazol-2-yl)-1-oxo-3-phenylpropan-2-ylamino]-1-oxo-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
-
NaCl
-
inhibition of rCPB2.8 and rCPB3 at pH higher than 5
Nalpha-(morpholin-4-ylcarbonyl)-N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-phenylalaninamide
-
-
Nalpha-(morpholin-4-ylcarbonyl)-N-[(2S)-1-oxo-4-phenyl-1-(5-phenyl-1,2-oxazol-3-yl)butan-2-yl]-L-phenylalaninamide
-
-
Nalpha-[(4-methylpiperazin-1-yl)carbonyl]-N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-phenylalaninamide
-
-
NPHAQFGITKFFDL-NH2
-
-
o-aminobenzoyl-FAARYLNGA-NH2
-
-
o-aminobenzoyl-FAARYLNGAQ-[N-(2,4-dinitrophenyl)]-ethylenediamine
-
-
o-aminobenzoyl-KLAFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLFFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLGFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLIFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLLFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLMFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLMFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
o-aminobenzoyl-KLNFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLPFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLQFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLRFSFQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
o-aminobenzoyl-KLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLRPSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLS(OBzl)FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLSFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLSKYLSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLSKYLSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
o-aminobenzoyl-KLTFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLVFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLWFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-KLYFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
-
o-aminobenzoyl-LKLLRKMQ-(N-[2,4-dinitrophenyl]-ethylenediamine)
-
-
o-aminobenzoyl-LKLLRKMQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
o-aminobenzoyl-MISLMKRPQ-[N-(2,4-dinitrophenyl)]-ethylenediamine
-
-
o-aminobenzoyl-RIIRIKRQ-(N-[2,4-dinitrophenyl]-ethylenediamine)
-
-
o-aminobenzoyl-RIIRIKRQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
o-aminobenzoyl-YRFFRNRFQ-(N-[2,4-dinitrophenyl]-ethylenediamine)
-
-
o-aminobenzoyl-YRFFRNRFQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
-
RYLNGAAYFAAAKR-NH2
-
-
SEAEFAARYLNGAA-NH2
-
-
trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane
-
1 microM, 37C, 48 h, pH 5.0
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
YFAAAKRHAAQHYR-NH2
-
-
additional information
-
high throughput screening of a compound library, identification of inhibitors containing the warhead-types semicarbazone, thiosemicarbazone and triazine nitrile, that can be used as leads for antiparasite drug design, docking study, overview
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
-
2 milliM, pH 5.0, 48 h, 37C, enzymatic activity depends on reducing agents
L-cysteine
-
2 milliM, pH 5.0, 48 h, 37C, enzymatic activity depends on reducing agents
NaCl
-
activation of rCPB2.8 and rCPB3 in acidic pH
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.058
2-aminobenzoyl-ELRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
-
0.021
2-aminobenzoyl-FLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
-
0.0006
2-aminobenzoyl-FRAK(Dnp)-NH2
-
pH 6.5, 37C
0.0024
2-aminobenzoyl-FRAK(Dnp)-OH
-
pH 6.5, 37C
0.435
2-aminobenzoyl-KERSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.024
2-aminobenzoyl-KFRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.041
2-aminobenzoyl-KGRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.044
2-aminobenzoyl-KLCSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.085
2-aminobenzoyl-KLESSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.084
2-aminobenzoyl-KLFSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.018
2-aminobenzoyl-KLHSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.038
2-aminobenzoyl-KLLSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.052
2-aminobenzoyl-KLNSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.106
2-aminobenzoyl-KLRESKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.043
2-aminobenzoyl-KLRFSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.038
2-aminobenzoyl-KLRGSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.035
2-aminobenzoyl-KLRNSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.103
2-aminobenzoyl-KLRRSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.059
2-aminobenzoyl-KLRSEKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.015
2-aminobenzoyl-KLRSFKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.052
2-aminobenzoyl-KLRSGKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.078
2-aminobenzoyl-KLRSLKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.029
2-aminobenzoyl-KLRSPKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.351
2-aminobenzoyl-KLRSRKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.029
2-aminobenzoyl-KLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.406
2-aminobenzoyl-KNRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.124
2-aminobenzoyl-KRRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.172
2-aminobenzoyl-LLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
-
0.029
2-aminobenzoyl-NLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
-
4.7
Abz-ARF(2,4-dinitrophenyl)-epsilon-NH2-Lys
-
rH84Y
0.0174
Abz-ARFK-OH
-
-
0.7 - 1.9
Abz-FR(2,4-dinitrophenyl)-epsilon-NH2-Lys
2.2 - 2.9
Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys
0.85 - 1.2
Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys-amide
0.001
Abz-FRAK-NH2
-
-
0.0036
Abz-FRAK-OH
-
-
3.3 - 3.9
Abz-FRF(2,4-dinitrophenyl)-epsilon-NH2-Lys
0.0036
Abz-FRFK-OH
-
-
0.0051
Abz-FRKP-OH
-
-
0.0016
Abz-FRKW-OH
-
-
0.001
Abz-RRFK-OH
-
-
0.005
Ac-Phe-Arg-7-amido-4-methylcoumarin
-
-
0.0114
benzoyl-(3-pyridylalanine)-R-4-methylcoumarin-7-amide
-
pH 6.3, 37C
0.021
benzoyl-(4-aminomethyl-N-isopropyl-phenylalanine)-R-4-methylcoumarin-7-amide
-
pH 6.3, 37C
0.0038
benzoyl-(4-aminomethyl-phenylalanine)-R-4-methylcoumarin-7-amide
-
pH 6.3, 37C
0.035
benzoyl-(4-guanidine-phenylalanine)-R-4-methylcoumarin-7-amide
-
pH 6.3, 37C
0.0014
benzoyl-FR-4-methylcoumarin-7-amide
-
pH 6.3, 37C
0.051
benzoyl-HR-4-methylcoumarin-7-amide
-
pH 6.3, 37C
0.012
benzoyl-RR-4-methylcoumarin-7-amide
-
pH 6.3, 37C
0.083
N-benzoyl-Pro-Phe-Arg-p-nitroanilide
-
pH 6.0, 37C
0.00013
o-aminobenzoyl-ALRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00044
o-aminobenzoyl-ANHFLCTQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00025
o-aminobenzoyl-HLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.0007
o-aminobenzoyl-KARFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00029
o-aminobenzoyl-KFRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00017
o-aminobenzoyl-KLFNPKFQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00016
o-aminobenzoyl-KLRASKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00011
o-aminobenzoyl-KLRFAKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00009
o-aminobenzoyl-KLRFFKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00016
o-aminobenzoyl-KLRFPKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00006
o-aminobenzoyl-KLRFRKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00011
o-aminobenzoyl-KLRFSAQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00004
o-aminobenzoyl-KLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.001
o-aminobenzoyl-KLRFSPQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00007
o-aminobenzoyl-KLRFSRQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00016
o-aminobenzoyl-KLRLSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00043
o-aminobenzoyl-KLRRSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.1
o-aminobenzoyl-KLTC(SBzl)FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.31
o-aminobenzoyl-KLTDFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.11
o-aminobenzoyl-KLTEFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.03
o-aminobenzoyl-KLTHFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.043
o-aminobenzoyl-KLTKFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.04
o-aminobenzoyl-KLTRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.05
o-aminobenzoyl-KLTT(OBzl)FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00113
o-aminobenzoyl-KPRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00044
o-aminobenzoyl-KRRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00012
o-aminobenzoyl-LLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
0.00019
o-aminobenzoyl-RLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
pH 5.5, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
496
2-aminobenzoyl-ELRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
-
424
2-aminobenzoyl-FLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
-
4.05
2-aminobenzoyl-FRAK(Dnp)-NH2
Leishmania braziliensis
-
pH 6.5, 37C
1.92
2-aminobenzoyl-FRAK(Dnp)-OH
Leishmania braziliensis
-
pH 6.5, 37C
360
2-aminobenzoyl-KLCSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
372
2-aminobenzoyl-KLESSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
572
2-aminobenzoyl-KLFSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
255
2-aminobenzoyl-KLHSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
130
2-aminobenzoyl-KLLSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
491
2-aminobenzoyl-KLNSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
631
2-aminobenzoyl-KLRESKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
543
2-aminobenzoyl-KLRFSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
322
2-aminobenzoyl-KLRGSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
286
2-aminobenzoyl-KLRNSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
375
2-aminobenzoyl-KLRRSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
614
2-aminobenzoyl-KLRSEKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
912
2-aminobenzoyl-KLRSFKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
276
2-aminobenzoyl-KLRSGKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
334
2-aminobenzoyl-KLRSLKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
567
2-aminobenzoyl-KLRSPKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
552
2-aminobenzoyl-KLRSRKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
pH 6.5, 37C
10 - 1026
2-aminobenzoyl-KLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
296
2-aminobenzoyl-LLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
-
583
2-aminobenzoyl-NLRSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
Leishmania braziliensis
-
-
0.004
Abz-ARF(2,4-dinitrophenyl)-epsilon-NH2-Lys
Leishmania mexicana
-
rH84Y
0.02
Abz-ARFK-OH
Leishmania mexicana
-
-
0.14 - 0.24
Abz-FR(2,4-dinitrophenyl)-epsilon-NH2-Lys
0.35 - 0.46
Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys
0.9 - 1.14
Abz-FRA(2,4-dinitrophenyl)-epsilon-NH2-Lys-amide
5.4
Abz-FRAK-NH2
Leishmania mexicana
-
-
1.4
Abz-FRAK-OH
Leishmania mexicana
-
-
0.23 - 0.51
Abz-FRF(2,4-dinitrophenyl)-epsilon-NH2-Lys
1.1
Abz-FRFK-OH
Leishmania mexicana
-
-
1.5
Abz-FRKP-OH
Leishmania mexicana
-
-
1
Abz-FRKW-OH
Leishmania mexicana
-
-
0.005
Abz-RRFK-OH
Leishmania mexicana
-
-
1.45
benzoyl-(3-pyridylalanine)-R-4-methylcoumarin-7-amide
Leishmania mexicana
-
pH 6.3, 37C
1.4
benzoyl-(4-aminomethyl-N-isopropyl-phenylalanine)-R-4-methylcoumarin-7-amide
Leishmania mexicana
-
pH 6.3, 37C
0.18
benzoyl-(4-aminomethyl-phenylalanine)-R-4-methylcoumarin-7-amide
Leishmania mexicana
-
pH 6.3, 37C
1.1
benzoyl-(4-guanidine-phenylalanine)-R-4-methylcoumarin-7-amide
Leishmania mexicana
-
pH 6.3, 37C
3.9
benzoyl-FR-4-methylcoumarin-7-amide
Leishmania mexicana
-
pH 6.3, 37C
0.3
benzoyl-HR-4-methylcoumarin-7-amide
Leishmania mexicana
-
pH 6.3, 37C
0.2
benzoyl-RR-4-methylcoumarin-7-amide
Leishmania mexicana
-
pH 6.3, 37C
17.8
N-benzoyl-Pro-Phe-Arg-p-nitroanilide
Leishmania mexicana
-
pH 6.0, 37C
2.18
o-aminobenzoyl-ALRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
1.58
o-aminobenzoyl-HLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
2.2
o-aminobenzoyl-KARFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
4.5
o-aminobenzoyl-KFRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
0.7
o-aminobenzoyl-KLFNPKFQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
2.4
o-aminobenzoyl-KLRASKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
0.98
o-aminobenzoyl-KLRFAKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
2.5
o-aminobenzoyl-KLRFFKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
1.9
o-aminobenzoyl-KLRFPKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
0.95
o-aminobenzoyl-KLRFRKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
1.4
o-aminobenzoyl-KLRFSAQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
1.1
o-aminobenzoyl-KLRFSPQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
1.7
o-aminobenzoyl-KLRFSRQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
2.3
o-aminobenzoyl-KLRLSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
2.35
o-aminobenzoyl-KLRRSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
2.05
o-aminobenzoyl-KLTC(SBzl)FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
1.48
o-aminobenzoyl-KLTCFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
0.27
o-aminobenzoyl-KLTDFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
0.85
o-aminobenzoyl-KLTEFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
1.1
o-aminobenzoyl-KLTHFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
1.35
o-aminobenzoyl-KLTKFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
2.6
o-aminobenzoyl-KLTRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
0.583
o-aminobenzoyl-KLTT(OBzl)FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
0.53
o-aminobenzoyl-KPRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
1.45
o-aminobenzoyl-KRRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
0.74
o-aminobenzoyl-LLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
1.98
o-aminobenzoyl-RLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
Leishmania mexicana
-
pH 5.5, 37C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000016
2-aminobenzoyl-KLPSSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.1
2-aminobenzoyl-KLRGSKQ-(N-(ethylenediamine)-2,4-dinitrophenyl amide)
-
pH 6.5, 37C
0.00004
2-phenyl-1H-indole-3-carbaldehyde thiosemicarbazone
-
truncated enzyme mutant CPB2.8DEKTACTE, pH 7.4, 22C
0.000005
3-[(1H-benzimidazol-2-yl)-2H-1-benzopyran-2-ylidene]-2-carbaldehyde semicarbazone
-
truncated enzyme mutant CPB2.8DEKTACTE, pH 7.4, 22C
0.00057
4-(cyclohexylamino)-6-[(2-methoxy-3-pyridinyl)amino]-1,3,5-triazine-2-carbonitrile
-
truncated enzyme mutant CPB2.8DEKTACTE, pH 7.4, 22C
0.0000074
4-methyl N-[(S)-1-oxo-1-[(S)-1-oxo-4-phenyl-1-(5-phenylisoxazol-3-yl)butan-2-ylamino]-3-phenylpropan-2-yl]piperazine-1-carboxamide
-
pH 5.0 30C
0.00004
5-(4-methoxy-phenyl)-2-furanyl-2-carbaldehyde thiosemicarbazone
-
truncated enzyme mutant CPB2.8DEKTACTE, pH 7.4, 22C
0.0076
benzoyl-(4-aminomethyl-cyclohexyl-alanine)-R-4-methylcoumarin-7-amide
-
pH 6.3, 37C
0.00081
EAEFAARYLNGAAYF-NH2
-
pH 5.5, 37C
0.004
FAARYLNGA
-
pH 5.5, 37C
0.0041
FAARYLNGA-NH2
-
pH 5.5, 37C
0.00045
FAARYLNGAQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.0017
ITKFFDLSEAEFAA-NH2
-
pH 5.5, 37C
0.000078
KLSKYLSK-NH2
-
pH 5.5, 37C
0.000043
LKLLRKM-NH2
-
pH 5.5, 37C
0.001706
N-((S)-1-[(S)-1-[1-(benzyloxymethyl)-1H-1,2,3-triazol-4-yl]-1-oxo-3-phenylpropan-2-ylamino]-1-oxo-3-phenylpropan-2-yl)morpholine-4-carboxamide
-
pH 5.0 30C
0.0000016
N-[(2S)-1-[(2S)-1-hydroxy-3-phenyl-1-(5-phenyl-1,2,4-oxadiazol-3-yl)propan-2-ylamino]-1-oxo-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
pH 5.0 30C
0.000014
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(1-phenyl-1H-1,2,3-triazol-4-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
one of two purified isomers, pH 5.0 30C
0.000048
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(3-phenylisoxazol-5-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
pH 5.0 30C
0.000004
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(5-phenylisoxazol-3-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
one of two purified isomers, pH 5.0 30C
0.000015
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(5-phenyloxazol-2-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
pH 5.0 30C
0.000417
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(5-phenylthiazol-2-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
pH 5.0 30C
0.000118
N-[(S)-1-[(d)-1-(5-benzylisoxazol-3-yl)-1-oxo-4-phenylbutan-2-ylamino]-1-oxo-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
pH 5.0 30C
0.000115
N-[(S)-1-[(S)-1-(benzo[d]oxazol-2-yl)-1-oxo-3-phenylpropan-2-ylamino]-1-oxo-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
pH 5.0 30C
0.000767
N-[(S)-1-[(S)-1-(benzo[d]thiazol-2-yl)-1-oxo-3-phenylpropan-2-ylamino]-1-oxo-3-phenylpropan-2-yl]morpholine-4-carboxamide
-
pH 5.0 30C
0.000006
Nalpha-(morpholin-4-ylcarbonyl)-N-[(2S)-1-oxo-4-phenyl-1-(5-phenyl-1,2-oxazol-3-yl)butan-2-yl]-L-phenylalaninamide
-
one of two purified isomers, pH 5.0 30C
0.0002
NPHAQFGITKFFDL-NH2
-
pH 5.5, 37C
0.00006
o-aminobenzoyl-FAARYLNGA-NH2
-
pH 5.5, 37C
0.00003
o-aminobenzoyl-FAARYLNGAQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000027
o-aminobenzoyl-KLAFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000018
o-aminobenzoyl-KLFFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000015 - 0.000018
o-aminobenzoyl-KLGFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
0.000009
o-aminobenzoyl-KLIFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000022
o-aminobenzoyl-KLMFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000032
o-aminobenzoyl-KLNFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.0004
o-aminobenzoyl-KLPFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000043
o-aminobenzoyl-KLQFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.00004
o-aminobenzoyl-KLRFSFQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.00049
o-aminobenzoyl-KLRPSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.00001
o-aminobenzoyl-KLS(OBzl)FSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000024
o-aminobenzoyl-KLSFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000007
o-aminobenzoyl-KLSKYLSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000016
o-aminobenzoyl-KLTFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000029
o-aminobenzoyl-KLVFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000028
o-aminobenzoyl-KLWFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000026
o-aminobenzoyl-KLYFSKQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000012
o-aminobenzoyl-LKLLRKMQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000008
o-aminobenzoyl-MISLMKRPQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000042
o-aminobenzoyl-RIIRIKRQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.000039
o-aminobenzoyl-YRFFRNRFQ-[N-(2,4-dinitrophenyl)-ethylenediamine]
-
pH 5.5, 37C
0.0001
RYLNGAAYFAAAKR-NH2
-
pH 5.5, 37C
0.0012
SEAEFAARYLNGAA-NH2
-
pH 5.5, 37C
0.00055
YFAAAKRHAAQHYR-NH2
-
pH 5.5, 37C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00007
(2(1H)-pyridinethionato-kappaS2)[2,6-bis[(mercapto-KappaS)methyl]pyridine-kappaN1] oxorhenium(V)
Leishmania major
-
-
0.01
(methanethiolato)[2,2'-(thio-kappaS)bis[ethanethiolato-kappaS]] oxorhenium (V)
Leishmania major
-
above 0.01 mM
0.001
(p-methoxyphenylthiolato-S)[2,6-bis[(mercapto-kappaS)methyl]pyridine-kappaN] oxorhenium(V)
Leishmania major
-
-
0.00006
2-phenyl-1H-indole-3-carbaldehyde thiosemicarbazone
Leishmania mexicana
-
truncated enzyme mutant CPB2.8DEKTACTE, pH 7.4, 22C
0.00001
3-[(1H-benzimidazol-2-yl)-2H-1-benzopyran-2-ylidene]-2-carbaldehyde semicarbazone
Leishmania mexicana
-
truncated enzyme mutant CPB2.8DEKTACTE, pH 7.4, 22C
0.0011
4-(cyclohexylamino)-6-[(2-methoxy-3-pyridinyl)amino]-1,3,5-triazine-2-carbonitrile
Leishmania mexicana
-
truncated enzyme mutant CPB2.8DEKTACTE, pH 7.4, 22C
0.00023
4-methyl N-[(S)-1-oxo-1-[(S)-1-oxo-4-phenyl-1-(5-phenylisoxazol-3-yl)butan-2-ylamino]-3-phenylpropan-2-yl]piperazine-1-carboxamide
Leishmania mexicana
-
pH 5.0 30C
0.00007
5-(4-methoxy-phenyl)-2-furanyl-2-carbaldehyde thiosemicarbazone
Leishmania mexicana
-
truncated enzyme mutant CPB2.8DEKTACTE, pH 7.4, 22C
0.0021
aceto[2,6-bis[(butylthio-jS)methyl]phenyl-kappaC]-,(SP-4-3)-palladium(II)
Leishmania major
-
-
0.0002
chloro[2,2'-(thio-kappaS)bis[ethanethiolato-kappaS]] oxorhenium(V)
Leishmania major
-
-
0.0017
diaceto[2-[(2-pyridinyl-kappaN)methyl]-phenyl-kappaC]Au(III)-,(SP-4-3)
Leishmania major
-
-
0.0529
N-((S)-1-[(S)-1-[1-(benzyloxymethyl)-1H-1,2,3-triazol-4-yl]-1-oxo-3-phenylpropan-2-ylamino]-1-oxo-3-phenylpropan-2-yl)morpholine-4-carboxamide
Leishmania mexicana
-
pH 5.0 30C
0.00005
N-[(2S)-1-[(2S)-1-hydroxy-3-phenyl-1-(5-phenyl-1,2,4-oxadiazol-3-yl)propan-2-ylamino]-1-oxo-3-phenylpropan-2-yl]morpholine-4-carboxamide
Leishmania mexicana
-
pH 5.0 30C
0.00044
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(1-phenyl-1H-1,2,3-triazol-4-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
Leishmania mexicana
-
one of two purified isomers, pH 5.0 30C
0.0015
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(3-phenylisoxazol-5-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
Leishmania mexicana
-
pH 5.0 30C
0.00013
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(5-phenylisoxazol-3-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
Leishmania mexicana
-
one of two purified isomers, pH 5.0 30C
0.00047
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(5-phenyloxazol-2-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
Leishmania mexicana
-
pH 5.0 30C
0.01294
N-[(S)-1-oxo-1-[(S)-1-oxo-3-phenyl-1-(5-phenylthiazol-2-yl)propan-2-ylamino]-3-phenylpropan-2-yl]morpholine-4-carboxamide
Leishmania mexicana
-
pH 5.0 30C
0.00366
N-[(S)-1-[(d)-1-(5-benzylisoxazol-3-yl)-1-oxo-4-phenylbutan-2-ylamino]-1-oxo-3-phenylpropan-2-yl]morpholine-4-carboxamide
Leishmania mexicana
-
pH 5.0 30C
0.00355
N-[(S)-1-[(S)-1-(benzo[d]oxazol-2-yl)-1-oxo-3-phenylpropan-2-ylamino]-1-oxo-3-phenylpropan-2-yl]morpholine-4-carboxamide
Leishmania mexicana
-
pH 5.0 30C
0.02379
N-[(S)-1-[(S)-1-(benzo[d]thiazol-2-yl)-1-oxo-3-phenylpropan-2-ylamino]-1-oxo-3-phenylpropan-2-yl]morpholine-4-carboxamide
Leishmania mexicana
-
pH 5.0 30C
0.000027
Nalpha-(morpholin-4-ylcarbonyl)-N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-phenylalaninamide
Leishmania mexicana
-
pH 5.0 30C
0.00019
Nalpha-(morpholin-4-ylcarbonyl)-N-[(2S)-1-oxo-4-phenyl-1-(5-phenyl-1,2-oxazol-3-yl)butan-2-yl]-L-phenylalaninamide
Leishmania mexicana
-
one of two purified isomers, pH 5.0 30C
0.000054
Nalpha-[(4-methylpiperazin-1-yl)carbonyl]-N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-phenylalaninamide
Leishmania mexicana
-
pH 5.0 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
room temperature, 45 min
6.5
-
substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
7.4
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 7.5
-
pH 4.5: about 70% of maximal activity, pH 7.5: about 45% of maximal activity, substrate: benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
-
assay at
26
-
reduced activity at 37 C
additional information
-
room temperature, 45 min, pH 5.5
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.34
-
isoenzyme CPB1
4.37
-
isoenzyme CPB19
4.83
-
isoenzyme CPB2.8
5.4
-
isoenzyme CPB18
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
attached to membrane domains via glycosylphophatidylinositol anchor
Manually annotated by BRENDA team
-
recombinant enzyme expressed in Escherichia coli; recombinant enzyme from Escherichia coli
Manually annotated by BRENDA team
-
promastigote enzyme
-
Manually annotated by BRENDA team
-
main amastigote enzymes
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26000
-
x * 26000, activated enzyme, SDS-PAGE
35000
-
SDS-PAGE
38000
-
Western blotting
40000
-
Western blotting
45000
-
SDS-PAGE
50000
-
SDS-PAGE, unstable
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 26000, activated enzyme, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
-
overexpression of CPB2.8 as an inactive pro-form lacking the characteristic C-terminal extension, CPB2.8DELTACTE in Escherichia coli. Pro-region processing is initiated during protein refolding and proceeds through several intermediate stages. Maximum enzyme activity after removal of the entire pro-region. The activated enzyme has a MW of 26000 Da, the inactive pro-form of the enzyme has a MW of 38000 Da as determined by SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 9
-
37C, decreased enzymatic activity at pH 3.0 or neutral-alkaline pH values, 48 h
697814
additional information
-
stable at basic pH
668458
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
pH 5.0, 48 h
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
37C, 48 h, pH 5.0
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from inclusion bodies
-
gel filtration and anion exchange chromatography
-
recombinant CPB2.3DELTACTE (CPB2.3 lacking the C-terminal extension)
-
Triton X-114 extraction, Con A-affinity chromatography, ion-exchange chromatography (DEAE-Sephacel)
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expression in Escherichia coli
-
expression of CPB2.3DELTACTE (truncated CPB2.3 lacking the C-terminal extension) in Escherichia coli
-
in Escherichia coli
-
overexpression of CPB2.8 as an inactive pro-form lacking the characteristic C-terminal extension, CPB2.8DELTACTE in Escherichia coli. Pro-region processing is initiated during protein refolding and proceeds through several intermediate stages. Maximum enzyme activity after removal of the entire pro-region. This is facilitated by acidification
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recombinant expression of the amastigote-specific isoform CPB2.8, expressed without the C-terminal extension, termed CPB2.8DEKTACTE
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subcloned into pET-23a for expression in Escherichia coli BL21 (DE3) pLysS cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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CBT2.8deltaCTE, CBT lacking the characteristic C-terminal extension
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine