Application | Comment | Organism |
---|---|---|
medicine | patients infected with a SARS-Cov-2 sub-lineage (B.1.1.284) carrying the Pro108Ser mutation in 3CLpro tend to have a comparatively milder clinical course than patients infected with the same sub-lineage of virus not carrying the mutation | Severe acute respiratory syndrome coronavirus 2 |
Protein Variants | Comment | Organism |
---|---|---|
P108S | patients infected with a SARS-Cov-2 sub-lineage (B.1.1.284) carrying the Pro108Ser mutation in 3CLpro tend to have a comparatively milder clinical course than patients infected with the same sub-lineage of virus not carrying the mutation. The Kcat/Km value of the 3CLpro enzyme containing Ser108 is 58% lower than that of Pro108 3CLpro. The reduced activity is associated with structural perturbation surrounding the substrate-binding region of the enzyme | Severe acute respiratory syndrome coronavirus 2 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GC376 | - |
Severe acute respiratory syndrome coronavirus 2 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
Dabcyl-KTSAVLQSGFRKME-EDANS | wild-type, pH 7.5, 25°C | Severe acute respiratory syndrome coronavirus 2 | |
0.216 | - |
Dabcyl-KTSAVLQSGFRKME-EDANS | mutant P108S, pH 7.5, 25°C | Severe acute respiratory syndrome coronavirus 2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome coronavirus 2 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Dabcyl-KTSAVLQSGFRKME-EDANS + H2O | - |
Severe acute respiratory syndrome coronavirus 2 | Dabcyl-KTSAVLQ + SGFRKME-EDANS | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
Dabcyl-KTSAVLQSGFRKME-EDANS | mutant P108S, pH 7.5, 25°C | Severe acute respiratory syndrome coronavirus 2 | |
0.0014 | - |
Dabcyl-KTSAVLQSGFRKME-EDANS | wild-type, pH 7.5, 25°C | Severe acute respiratory syndrome coronavirus 2 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00193 | - |
wild-type, pH 7.5, 25°C | Severe acute respiratory syndrome coronavirus 2 | GC376 | |
0.00374 | - |
mutant P108S, pH 7.5, 25°C | Severe acute respiratory syndrome coronavirus 2 | GC376 |