Protein Variants | Comment | Organism |
---|---|---|
A252S | A252S mutation in 3CLpro confers an 1.15fold increase in the IC50 value for the inhibitor GC376 and a 1.24fold increase in the IC50 value for the inhibitor NPI52 | feline coronavirus |
K260N | K260N mutation in 3CLpro confers an 1.05fold increase in the IC50 value for the inhibitor GC376 | feline coronavirus |
additional information | comparison of the amino acid sequences of feline coronavirus 3CLpro from the pre-treatment ascites sample and the postmortem tissue samples of Felis catus shows amino acid changes of N25S and K260N in the kidney and N25S, A252S and K260N in the lung and spleen | feline coronavirus |
N25S | N25S mutation in 3CLpro confers an 1.38fold increase in the IC50 value for the inhibitor GC376 and a 1.54fold increase in the IC50 value for the inhibitor NPI52 | feline coronavirus |
N25S/A252S/K260N | N25S/A252S/K260N mutation in 3CLpro confers an 1.68fold increase in the IC50 value for the inhibitor GC376 and a 1.06fold increase in the IC50 value for the inhibitor NPI52 | feline coronavirus |
N25S/K260N | N25S/K260N mutation in 3CLpro confers an 1.53fold increase in the IC50 value for the inhibitor GC376 and a 1.33fold increase in the IC50 value for the inhibitor NPI52 | feline coronavirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GC376 | peptidomimetic antiviral drug. A single amino acid change of N25S, but not A252S or K260N, leads to a statistically significant increase in the IC50 value as compared to that of wild-typwe enzyme | feline coronavirus | |
NPI52 | - |
feline coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
feline coronavirus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Dabcyl-KTSAVLQSGFRKME-EDANS + H2O | - |
feline coronavirus | Dabcyl-KTSAVLQ + SGFRKME-EDANS | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3cLpro | - |
feline coronavirus |
coronavirus 3C-like protease | - |
feline coronavirus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | feline coronavirus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | feline coronavirus |