Cloned (Comment) | Organism |
---|---|
wild-type enzyme and C-terminally truncated proteases, expression in Escherichia coli | Severe acute respiratory syndrome-related coronavirus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | truncation of C-terminus from 306 to 300 has no appreciable effect on the quaternary structure, and the enzyme remains catalytically active. Further deletion of Gln299 or Arg298 drastically decreases the enzyme activity to 12% of wild type, and the major form is a monomeric one. The catalytic constant and specificity constant (kcat/Km) of the proteases are significantly decreased in the DELTA(299306), DELTA(298306), and DELTA(297306) mutants. Wild type and DELTA(300306) proteases exist with dimer as the major form. The major form becomes monomeric in DELTA(299306), DELTA(298306) and DELTA(297306) | Severe acute respiratory syndrome-related coronavirus |
Q299A | the quaternary structures of exists in a mixture of monomeric and dimeric forms | Severe acute respiratory syndrome-related coronavirus |
Q299E | more than 90% loss of activity | Severe acute respiratory syndrome-related coronavirus |
Q299K | more than 90% loss of activity | Severe acute respiratory syndrome-related coronavirus |
Q299N | more than 90% loss of activity | Severe acute respiratory syndrome-related coronavirus |
R298A | the quaternary structures of exists in a mixture of monomeric and dimeric forms | Severe acute respiratory syndrome-related coronavirus |
R298A/Q299A | mutant is present almost exclusively in the monomeric form | Severe acute respiratory syndrome-related coronavirus |
R298K | mutation has no significant effect on activity | Severe acute respiratory syndrome-related coronavirus |
R298L | mutation destroys 85% of the enzyme activity | Severe acute respiratory syndrome-related coronavirus |
S123A | mutation does not destroy the enzyme activity, the dimeric structure remains intact | Severe acute respiratory syndrome-related coronavirus |
S123C | mutation does not destroy the enzyme activity, the dimeric structure remains intact | Severe acute respiratory syndrome-related coronavirus |
S139A | mutation can destroy neither the enzyme activity nor the dimeric structure | Severe acute respiratory syndrome-related coronavirus |
S139A | mutation does not destroy the enzyme activity, the dimeric structure remains intact | Severe acute respiratory syndrome-related coronavirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(303-306) | Severe acute respiratory syndrome-related coronavirus | |
0.005 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme Q200E | Severe acute respiratory syndrome-related coronavirus | |
0.005 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme Q299N | Severe acute respiratory syndrome-related coronavirus | |
0.006 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(304-306) | Severe acute respiratory syndrome-related coronavirus | |
0.01 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme R298A/Q299A | Severe acute respiratory syndrome-related coronavirus | |
0.01 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme S139A | Severe acute respiratory syndrome-related coronavirus | |
0.011 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | wild-type enzyme | Severe acute respiratory syndrome-related coronavirus | |
0.011 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme R298A | Severe acute respiratory syndrome-related coronavirus | |
0.012 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme S123a/R298A | Severe acute respiratory syndrome-related coronavirus | |
0.013 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(299-306) | Severe acute respiratory syndrome-related coronavirus | |
0.013 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme R298L | Severe acute respiratory syndrome-related coronavirus | |
0.013 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme S123A | Severe acute respiratory syndrome-related coronavirus | |
0.013 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme S123C | Severe acute respiratory syndrome-related coronavirus | |
0.014 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(305-306) | Severe acute respiratory syndrome-related coronavirus | |
0.015 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(300-306) | Severe acute respiratory syndrome-related coronavirus | |
0.016 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme R298K | Severe acute respiratory syndrome-related coronavirus | |
0.018 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme Q299A | Severe acute respiratory syndrome-related coronavirus | |
0.021 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(297-306) | Severe acute respiratory syndrome-related coronavirus | |
0.021 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme S139A/Q299A | Severe acute respiratory syndrome-related coronavirus | |
0.022 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(302-306) | Severe acute respiratory syndrome-related coronavirus | |
0.03 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(298-306) | Severe acute respiratory syndrome-related coronavirus | |
0.03 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(301-306) | Severe acute respiratory syndrome-related coronavirus | |
0.037 | - |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme Q299K | Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type enzyme and C-terminally truncated proteases | Severe acute respiratory syndrome-related coronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide + H2O | - |
Severe acute respiratory syndrome-related coronavirus | 2-aminobenzoyl-TSAVLQ + SGFRK-2,4-dinitrophenyl amide | - |
? | |
o-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide + H2O | - |
Severe acute respiratory syndrome-related coronavirus | o-aminobenzoyl-TSAVLQSGFRK + 2,4-dinitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | wild type and DELTA(300306) proteases exist with dimer as the major form. The major form becomes monomeric in DELTA(299306), DELTA(298306) and DELTA(297306) | Severe acute respiratory syndrome-related coronavirus |
monomer | wild type and DELTA(300306) proteases exist with dimer as the major form. The major form becomes monomeric in DELTA(299306), DELTA(298306) and DELTA(297306) | Severe acute respiratory syndrome-related coronavirus |
Synonyms | Comment | Organism |
---|---|---|
SARS-CoV main protease | - |
Severe acute respiratory syndrome-related coronavirus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(297-306) | Severe acute respiratory syndrome-related coronavirus | |
0.00021 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme R298A/Q299A | Severe acute respiratory syndrome-related coronavirus | |
0.0006 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(298-306) | Severe acute respiratory syndrome-related coronavirus | |
0.0007 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme Q299A | Severe acute respiratory syndrome-related coronavirus | |
0.001 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(299-306) | Severe acute respiratory syndrome-related coronavirus | |
0.0017 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme Q299N | Severe acute respiratory syndrome-related coronavirus | |
0.0021 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme Q299K | Severe acute respiratory syndrome-related coronavirus | |
0.0022 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme Q200E | Severe acute respiratory syndrome-related coronavirus | |
0.0034 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme R298A | Severe acute respiratory syndrome-related coronavirus | |
0.0048 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme R298L | Severe acute respiratory syndrome-related coronavirus | |
0.0048 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme S139A/Q299A | Severe acute respiratory syndrome-related coronavirus | |
0.0069 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme S123a/R298A | Severe acute respiratory syndrome-related coronavirus | |
0.017 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(300-306) | Severe acute respiratory syndrome-related coronavirus | |
0.017 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme R298K | Severe acute respiratory syndrome-related coronavirus | |
0.022 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(303-306) | Severe acute respiratory syndrome-related coronavirus | |
0.025 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(301-306) | Severe acute respiratory syndrome-related coronavirus | |
0.027 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(304-306) | Severe acute respiratory syndrome-related coronavirus | |
0.03 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(302-306) | Severe acute respiratory syndrome-related coronavirus | |
0.032 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | wild-type enzyme | Severe acute respiratory syndrome-related coronavirus | |
0.033 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme S123A | Severe acute respiratory syndrome-related coronavirus | |
0.033 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme S139A | Severe acute respiratory syndrome-related coronavirus | |
0.035 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme S123C | Severe acute respiratory syndrome-related coronavirus | |
0.039 | - |
2-aminobenzoyl-TSAVLQSGFRK-2,4-dinitrophenyl amide | mutant enzyme DELTA(305-306) | Severe acute respiratory syndrome-related coronavirus |