Cloned (Comment) | Organism |
---|---|
expression analysis by realtime PCR, regulation of enzyme expression takes place at the transcriptional level, transcriptional transactivation of the NF-kappaB-responsive promoter which is higher in cells with mitochondria depleted in mtDNA | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | cathepsin B is secreted as an inactive precursor form, up-regulation of cathepsin B expression and enhanced secretion in mitochondrial DNA-depleted osteosarcoma cells resulting, at least in part, from the activation of the transcription factor NF-kappaB | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, enzyme precursor form, SDS-PAGE, x * 30000-31000, intermediate single-chain enzyme form, SDS-PAGE, x* 24000-25000, mature double-chain enzyme form, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | NF-kappaB induced up-regulation of cathepsin B expression contributes to the invasive property of the 143B cells lacking mtDNA, overview. Extracellularly released cathepsin B can act alone or in concert with some matrix metalloproteinases | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | cathepsin B is secreted as an inactive precursor form and activated by cleavage through pepsin at pH 3.5 | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
143B cell | - |
Homo sapiens | - |
osteosarcoma cell | up-regulation of cathepsin B expression and enhanced secretion in mitochondrial DNA-depleted osteosarcoma cells resulting, at least in part, from the activation of the transcription factor NF-kappaB | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NF-kappaB induced up-regulation of cathepsin B expression contributes to the invasive property of the 143B cells lacking mtDNA, overview. Extracellularly released cathepsin B can act alone or in concert with some matrix metalloproteinases | Homo sapiens | ? | - |
? | |
Nalpha-benzoyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Nalpha-benzoyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, enzyme precursor form, SDS-PAGE, x * 30000-31000, intermediate single-chain enzyme form, SDS-PAGE, x* 24000-25000, mature double-chain enzyme form, SDS-PAGE | Homo sapiens |
More | peptide mapping in 143B cells by 2D gel electrophoresis and mass spectrometry | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | multiple forms of cathepsin B derived peptides with pIs ranging from a pI of 5.5 to a pI of 5.9 | 5.9 | 5.5 |