Cloned (Comment) | Organism |
---|---|
expression of NS2B(H)-NS3pro complex in E.scherichiacoli | Dengue virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
acetyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide | pH 9.5, 37°C | Dengue virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10000 | - |
1 * 21000 plus 1 * 10000, SDS-PAGE, corresponding to the NS3pro protease domain only, and the His-tagged NS2B(H) cofactor | Dengue virus |
21000 | - |
1 * 21000 plus 1 * 10000, SDS-PAGE, corresponding to the NS3pro protease domain only, and the His-tagged NS2B(H) cofactor | Dengue virus |
37000 | - |
x * 37000, SDS-PAGE, intact NS2B(H)-NS3pro complex | Dengue virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dengue virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
expression of NS2B(H)-NS3pro complex in E.scherichiacoli predominantly as insoluble protein and purification to 95% purity | Dengue virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide + H2O | - |
Dengue virus | acetyl-Nle-Lys-Arg-Arg + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, SDS-PAGE, intact NS2B(H)-NS3pro complex | Dengue virus |
dimer | 1 * 21000 plus 1 * 10000, SDS-PAGE, corresponding to the NS3pro protease domain only, and the His-tagged NS2B(H) cofactor | Dengue virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.112 | - |
acetyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide | pH 9.5, 37°C | Dengue virus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.12 | - |
acetyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide | pH 9.5, 37°C | Dengue virus |