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Literature summary for 3.4.21.82 extracted from

  • Balaban, N.P.; Mardanova, A.M.; Sharipova, M.R.; Gabdrakhmanova, L.A.; Sokolova, E.A.; Garusov, A.V.; Milgotina, E.I.; Rudenskaya, G.N.; Leshchinskaya, I.B.
    Isolation and characterization of glutamyl endopeptidase 2 from Bacillus intermedius 3-19 (2003), Biochemistry (Moscow), 68, 1217-1224.
    View publication on PubMed
Search for more literature for 3.4.21.82

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate
-
 Bacillus intermedius
additional information little or no inhibition with PMSF, EDTA, benzamidine, p-CMB, duck ovomucoid, inhibitor from marine anemone, and soybean trypsin inhibitor Bacillus intermedius

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stabilizes Bacillus intermedius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26500
-
 gel filtration Bacillus intermedius

Organism

Organism UniProt Comment Textmining
Bacillus intermedius
-
 strain 3-19
-
Bacillus intermedius Mrz 19
-
 strain 3-19
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzlyoxycarbonyl-Ala-Ala-Leu-Glu-p-nitroanilide + H2O
-
 Bacillus intermedius ?
-
 ?
benzlyoxycarbonyl-Ala-Ala-Met-Glu-p-nitroanilide + H2O
-
 Bacillus intermedius ?
-
 ?
benzlyoxycarbonyl-Ala-Ala-Met-Glu-p-nitroanilide + H2O
-
 Bacillus intermedius Mrz 19 ?
-
 ?
benzlyoxycarbonyl-Ala-Ala-Phe-Glu-p-nitroanilide + H2O
-
 Bacillus intermedius ?
-
 ?
benzlyoxycarbonyl-Ala-Ala-Trp-Glu-p-nitroanilide + H2O
-
 Bacillus intermedius ?
-
 ?
benzlyoxycarbonyl-Ala-Ala-Trp-Glu-p-nitroanilide + H2O
-
 Bacillus intermedius Mrz 19 ?
-
 ?
benzlyoxycarbonyl-Glu-p-nitroanilide + H2O
-
 Bacillus intermedius ?
-
 ?
benzlyoxycarbonyl-Gly-Ala-Ala-Glu-p-nitroanilide + H2O
-
 Bacillus intermedius ?
-
 ?
casein + H2O
-
 Bacillus intermedius hydrolyzed casein
-
 ?
casein + H2O
-
 Bacillus intermedius Mrz 19 hydrolyzed casein
-
 ?
insulin + H2O hydrolyzes Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12 bonds in the oxidized A-chain of insulin and Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20 bonds in the oxidized B-chain of insulin Bacillus intermedius ?
-
 ?
insulin + H2O hydrolyzes Glu4-Gln5, Glu17-Asp18, and Cys11-Ser12 bonds in the oxidized A-chain of insulin and Glu13-Ala14, Glu21-Arg22, Cys7-Gly8, and Cys19-Gly20 bonds in the oxidized B-chain of insulin Bacillus intermedius Mrz 19 ?
-
 ?
additional information does not hydrolyze the subtilisin substrate benzyloxycarbonyl-Ala-Ala-Leu-p-nitroanilide, only little activity with benzyloxycarbonyl-Ala-Ala-Trp-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Leu-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Met-Asp-p-nitroanilide, and benzyloxycarbonyl-Gly-Ala-Ala-Asp-p-nitroanilide Bacillus intermedius ?
-
 ?
additional information does not hydrolyze the subtilisin substrate benzyloxycarbonyl-Ala-Ala-Leu-p-nitroanilide, only little activity with benzyloxycarbonyl-Ala-Ala-Trp-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Leu-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-Asp-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Met-Asp-p-nitroanilide, and benzyloxycarbonyl-Gly-Ala-Ala-Asp-p-nitroanilide Bacillus intermedius Mrz 19 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26500, SDS-PAGE Bacillus intermedius

Synonyms

Synonyms Comment Organism
glutamyl endopeptidase 2
-
 Bacillus intermedius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22 37 without Ca2+, stable at 37°C, but activity decreases at 50°C and 55°C by 55% and 90% respectively Bacillus intermedius
37 55 with Ca2+, highly thermostable, heating at 37°C, 50°C, and 55°C has no effect on activity Bacillus intermedius