Information on EC 3.4.21.82 - Glutamyl endopeptidase II

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.21.82
-
RECOMMENDED NAME
GeneOntology No.
Glutamyl endopeptidase II
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage: -Glu-/- >> -Asp-/-. Preference for Pro or Leu at P2 and Phe at P3. Cleavage of -Glu-/-Asp- and -Glu-/-Pro- bonds is slow
show the reaction diagram
preferential cleavage:-Glu-+- >>-Asp-+-. Preference for Pro or Leu at P2 and Phe at P3. Cleavage of-Glu-+-Asp and-Glu-+-Pro- bonds is slow, the term-+- depicts the point of cleavage
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
137010-42-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 3-19
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-
Manually annotated by BRENDA team
strain 3-19
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-Aminobenzoyl-Ala-Ala-Glu-Val-Tyr(NO2)-Asp-OH + H2O
2-Aminobenzoyl-Ala-Ala-Glu + Val-Tyr(NO2)-Asp-OH
show the reaction diagram
-
-
-
-
-
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
benzlyoxycarbonyl-Ala-Ala-Leu-Glu-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
benzlyoxycarbonyl-Ala-Ala-Met-Glu-p-nitroanilide + H2O
?
show the reaction diagram
benzlyoxycarbonyl-Ala-Ala-Phe-Glu-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
benzlyoxycarbonyl-Ala-Ala-Trp-Glu-p-nitroanilide + H2O
?
show the reaction diagram
benzlyoxycarbonyl-Glu-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
benzlyoxycarbonyl-Gly-Ala-Ala-Glu-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Gly-Leu-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Ile-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Leu-Ala-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Leu-GLy-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Leu-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Met-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-2-carboxyphenyl thioester + Nva-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Gly-Leu-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Ile-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Leu-Ala-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Leu-Gly-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Leu-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Met-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-3-carboxyphenyl ester + Nva-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Gly-Leu-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Ile-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Leu-Ala-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Leu-Gly-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Leu-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Met-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-4-carboxyphenyl ester + Nva-NH2
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Gly-Leu-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Ile-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Leu-Ala-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Leu-Gly-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Leu-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Met-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxyethyl thioester + Nva-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Gly-Leu-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Ile-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Leu-Ala-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Leu-Gly-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Leu-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Met-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Ala-carboxymethyl thioester + Nva-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Gly-Leu-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Ile-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Leu-Ala-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Leu-Gly-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Leu-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Met-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Asp-methyl ester + Nva-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Gly-Leu-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Ile-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Leu-Ala-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Leu-Gly-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Leu-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Met-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl ester + Nva-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Gly-Leu-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Ile-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Leu-Ala-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Leu-Gly-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Leu-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Met-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
benzyloxycarbonyl-Glu-methyl thioester + Nva-NH2
?
show the reaction diagram
-
protease catalyzed acyltransfer from substrates to amino acid amides
-
?
Benzyloxycarbonyl-Leu-Leu-Glu 2-naphthylamide + H2O
Benzyloxycarbonyl-Leu-Leu-Glu + 2-naphthylamine
show the reaction diagram
-
-
-
-
-
Benzyloxycarbonyl-Phe-Leu-Glu 2-nitroanilide + H2O
Benzyloxycarbonyl-Phe-Leu-Glu + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Bovine serum albumin + H2O
?
show the reaction diagram
-
BSA
-
-
?
casein + H2O
hydrolyzed casein
show the reaction diagram
insulin + H2O
?
show the reaction diagram
S-AAPEpNA + H2O
?
show the reaction diagram
-
specific SGPE substrate
-
-
?
Synthetic peptide substrates containing an N-terminal anthraniloyl group and a 3-nitrotyrosine close to the C-terminus + H2O
?
show the reaction diagram
-
-
-
-
-
tert-butoxycarbonyl-Ala-Ala-Pro-Asp 4-nitroanilide + H2O
tert-butoxycarbonyl-Ala-Ala-Pro-Asp + 4-nitroaniline
show the reaction diagram
-
slowly
-
-
-
tert-butoxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide + H2O
tert-butoxycarbonyl-Ala-Ala-Pro-Glu + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
tert-butoxycarbonyl-Ala-Phe-Pro-Glu 4-nitroanilide + H2O
tert-butoxycarbonyl-Ala-Phe-Pro-Glu + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Tert-Butyloxycarbonyl-Ala-Ala-Ala-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Ala-Ala-Glu + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Tert-Butyloxycarbonyl-Ala-Ala-Gly-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Ala-Gly-Glu + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Tert-Butyloxycarbonyl-Ala-Ala-Phe-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Ala-Phe-Glu + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Tert-Butyloxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Ala-Pro-Glu + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Tert-Butyloxycarbonyl-Ala-Leu-Pro-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Leu-Pro-Glu + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Tert-Butyloxycarbonyl-Ala-Phe-Pro-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Phe-Pro-Glu + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
Tert-Butyloxycarbonyl-Ala-Pro-Glu 4-nitroanilide + H2O
Tert-Butyloxycarbonyl-Ala-Pro-Glu + 4-nitroaniline
show the reaction diagram
-
-
-
-
-
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
stabilizes
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Chloromethane analogs of glutamic and aspartic acid-containing substrates
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-
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diisopropylfluorophosphate
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Inhibitor from the seeds of bitter gourd
-
-
-
PCMB
-
partial
RTI-2
-
proteinase inhibitor in rapeseed seeds
-
tosylphenylalanine chloromethyl ketone
-
partial
[Leu18-->Glu]-modified ovomucoid third domain
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strong
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.9
tert-butyloxycarbonyl-Ala-Ala-Ala-Glu 4-nitroanilide
-
-
3.98
tert-butyloxycarbonyl-Ala-Ala-Gly-Glu 4-nitroanilide
-
-
1.13
tert-butyloxycarbonyl-Ala-Ala-Leu-Glu 4-nitroanilide
-
-
1.39
Tert-Butyloxycarbonyl-Ala-Ala-Phe-Glu 4-nitroanilide
-
-
0.484
tert-butyloxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide
-
-
0.599
Tert-Butyloxycarbonyl-Ala-Leu-Pro-Glu 4-nitroanilide
-
-
0.407
Tert-Butyloxycarbonyl-Ala-Phe-Pro-Glu 4-nitroanilide
-
-
3.25
Tert-Butyloxycarbonyl-Ala-Pro-Glu 4-nitroanilide
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
473
tert-butyloxycarbonyl-Ala-Ala-Ala-Glu 4-nitroanilide
Streptomyces griseus
-
-
30
tert-butyloxycarbonyl-Ala-Ala-Gly-Glu 4-nitroanilide
Streptomyces griseus
-
-
179
tert-butyloxycarbonyl-Ala-Ala-Leu-Glu 4-nitroanilide
Streptomyces griseus
-
-
365
Tert-Butyloxycarbonyl-Ala-Ala-Phe-Glu 4-nitroanilide
Streptomyces griseus
-
-
5390
tert-butyloxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide
Streptomyces griseus
-
-
500
Tert-Butyloxycarbonyl-Ala-Leu-Pro-Glu 4-nitroanilide
Streptomyces griseus
-
-
381
Tert-Butyloxycarbonyl-Ala-Phe-Pro-Glu 4-nitroanilide
Streptomyces griseus
-
-
182
Tert-Butyloxycarbonyl-Ala-Pro-Glu 4-nitroanilide
Streptomyces griseus
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.044
RTI-2
-
competitive inhibition, titration curve
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.8
-
tert-butoxycarbonyl-Ala-Ala-Pro-Glu 4-nitroanilide
additional information
-
pI: 8.4
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18270
-
Streptomyces griseus, amino acid sequence
20000
-
Streptomyces griseus, gel filtration
22000
-
1 * 22000, Streptomyces griseus, SDS-PAGE in presence of 2-mercaptoethanol
26500
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
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relatively stable
29778
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22 - 37
-
without Ca2+, stable at 37C, but activity decreases at 50C and 55C by 55% and 90% respectively
37 - 55
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with Ca2+, highly thermostable, heating at 37C, 50C, and 55C has no effect on activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
of the recombinant protein
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Bacillus subtilis
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
peptide coupling catalyzed by proteasesis an alternative to chemical solution and solid phase peptide synthesis