Cloned (Comment) | Organism |
---|---|
recombinant expression of mutant enzyme | Homo sapiens |
recombinant expression of wild-type enzyme | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme mutant H99Y in complex with mupain-1 inhibitor variants, sitting drop vapour diffusion method, equilibrating of protein against a reservoir solution containing 2.0 M ammonium sulfate, 50 mM sodium citrate, pH 4.6, and 5% PEG 400, room temperature, about 3 days, soaking of crystals in 40% PEG 4000, 100 mM Tris-HCl, pH 7.4 containing 1 mM mupain ligand variant, X-ray diffraction structure determination and analysis at 1.6 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H99Y | site-directed mutagenesis, structure analysis with bound inhibitors | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(1-carbamimidoylpiperidin-3-yl)-L-alanine | - |
Homo sapiens | |
3-(1-carbamimidoylpiperidin-3-yl)-L-alanine | - |
Mus musculus | |
3-(1-carbamimidoylpiperidin-4-yl)-L-alanine | - |
Homo sapiens | |
3-(1-carbamimidoylpiperidin-4-yl)-L-alanine | - |
Mus musculus | |
mupain-1 | i.e. CPAYSRYLDC | Homo sapiens | |
mupain-1 | i.e. CPAYSRYLDC | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mutant enzyme by dialysis and ultrafiltration | Homo sapiens |
recombinant wild-type enzyme by dialysis and ultrafiltration | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
uPA | - |
Mus musculus |
uPA | - |
Homo sapiens |
Urokinase-type plasminogen activator | - |
Mus musculus |
Urokinase-type plasminogen activator | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
7.4 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics of mupain variants, overview | Mus musculus | |
additional information | - |
additional information | inhibition kinetics of mupain variants, overview | Homo sapiens | |
0.00248 | - |
3-(1-carbamimidoylpiperidin-4-yl)-L-alanine | pH 7.4, 37°C, enzyme mutant H99Y | Homo sapiens | |
0.0065 | - |
3-(1-carbamimidoylpiperidin-3-yl)-L-alanine | pH 7.4, 37°C, wild-type enzyme | Mus musculus | |
0.045 | - |
3-(1-carbamimidoylpiperidin-4-yl)-L-alanine | pH 7.4, 37°C, wild-type enzyme | Mus musculus | |
0.219 | - |
3-(1-carbamimidoylpiperidin-3-yl)-L-alanine | pH 7.4, 37°C, enzyme mutant H99Y | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme's catalytic domain comprises residues 159-411 | Mus musculus |
additional information | the enzyme's catalytic domain comprises residues 159-411 | Homo sapiens |