KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
N-alpha-benzoyl-L-arginine-p-nitroanilide | isozyme TLE II, at 37°C, pH 8.0 | Euphausia pacifica | |
0.09 | - |
N-alpha-benzoyl-L-arginine-p-nitroanilide | isozyme TLE I, at 37°C, pH 8.0 | Euphausia pacifica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates at concentrations above 0.4 mM | Euphausia pacifica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32300 | - |
TLE II, SDS-PAGE | Euphausia pacifica |
33000 | - |
TLE I, SDS-PAGE | Euphausia pacifica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euphausia pacifica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose FF column chromatography and Sephacryl S100 gel filtration | Euphausia pacifica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.52 | - |
crude extract, at 37°C, pH 8.0 | Euphausia pacifica |
64 | - |
isozyme TLE I after 123fold purification, at 37°C, pH 8.0 | Euphausia pacifica |
92 | - |
isozyme TLE II after 177fold purification, at 37°C, pH 8.0 | Euphausia pacifica |
Storage Stability | Organism |
---|---|
25°C, crude extract, 30 days, retains more than 70% of the initial activity | Euphausia pacifica |
40°C, crude extract, 5-10 days, retains about 80% of the initial activity | Euphausia pacifica |
40°C, purified enzyme, 30 min, remains stable | Euphausia pacifica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O | - |
Euphausia pacifica | N-alpha-benzoyl-L-arginine + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TLE I | - |
Euphausia pacifica |
TLE II | - |
Euphausia pacifica |
trypsin-like enzyme | - |
Euphausia pacifica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | - |
Euphausia pacifica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 70 | - |
Euphausia pacifica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
activity is lost rapidly over 50°C | Euphausia pacifica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.84 | - |
N-alpha-benzoyl-L-arginine-p-nitroanilide | isozyme TLE I, at 37°C, pH 8.0 | Euphausia pacifica | |
0.98 | - |
N-alpha-benzoyl-L-arginine-p-nitroanilide | isozyme TLE II, at 37°C, pH 8.0 | Euphausia pacifica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | - |
Euphausia pacifica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 11 | - |
Euphausia pacifica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Euphausia pacifica | TLE II, isoelectric focusing | - |
4.3 |
Euphausia pacifica | TLE I, isoelectric focusing | - |
4.5 |