Information on EC 3.4.21.4 - trypsin

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.4.21.4
-
RECOMMENDED NAME
GeneOntology No.
trypsin
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
preferential cleavage: Arg-/-, Lys-/-
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide hydrolysis
-
-
hydrolysis of peptide bond
-
-
endopeptidase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9002-07-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
Cambarus affinis
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Sugarcane rootstalk borer weevil
UniProt
Manually annotated by BRENDA team
sharpsnout sea bream
-
-
Manually annotated by BRENDA team
isoform EFE-b
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
putative trypsin
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
tobacco hornworm moth
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
diamondback moth
-
-
Manually annotated by BRENDA team
red palm weevil
-
-
Manually annotated by BRENDA team
salmon
-
-
-
Manually annotated by BRENDA team
Atlantic bonito
-
-
Manually annotated by BRENDA team
trypsin isozymes A, B and C
-
-
Manually annotated by BRENDA team
isoform TR-S
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
insecticide-resistant and -susceptible strains, serine proteinases with trypsin-like activity
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
yellowfin tuna
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Triops sp.
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(benzyloxycarbonyl-L-alanyl-L-arginine)2-rhodamine-110 + 2 H2O
2 benzyloxycarbonyl-L-alanyl-L-arginine + rhodamine-110
show the reaction diagram
-
specific trypsin substrate
-
-
?
4-methylumbelliferyl-4-guanidinobenzoate + H2O
4-methylumbelliferol + 4-guanidinobenzoate
show the reaction diagram
-
-
-
-
?
Abz-ERFK-EDDnp + H2O
Abz-ER + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-FRFK-EDDnp + H2O
Abz-FR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-GRFK-EDDnp + H2O
Abz-GR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-HRFK-EDDnp + H2O
Abz-HR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KRFK-EDDnp + H2O
Abz-KR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-LRFK-EDDnp + H2O
Abz-LR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-NRFK-EDDnp + H2O
Abz-NR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-QRFK-EDDnp + H2O
Abz-QR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RDFK-EDDnp + H2O
Abz-R + DFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-REFK-EDDnp + H2O
Abz-R + EFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RGFK-EDDnp + H2O
Abz-R + GFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RHFK-EDDnp + H2O
Abz-R + HFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RNFK-EDDnp + H2O
Abz-R + NFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RPFK-EDDnp + H2O
Abz-R + PFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RQFK-EDDnp + H2O
Abz-R + QFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RRFK-EDDnp + H2O
Abz-R + RFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RSFK-EDDnp + H2O
Abz-R + SFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RTFK-EDDnp + H2O
Abz-R + TFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RVFK-EDDnp + H2O
Abz-R + VFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-RYFK-EDDnp + H2O
Abz-R + YFK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-VRFK-EDDnp + H2O
Abz-VR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-WRFK-EDDnp + H2O
Abz-WR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-YRFK-EDDnp + H2O
Abz-YR + FK-EDDnp
show the reaction diagram
-
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg(NO2)-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Lys-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-N-omega-hydroxy-L-arginine-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-CN)-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Phe(p-NH2)-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
acetyl-L-Phe-L-Ala-L-Thr-(4-guanidino)-L-Phe-NH-(3-carbamoyl-4-nitrophenol) + H2O
acetyl-L-Phe-L-Ala-L-Thr-(4-guanidino)-L-Phe + 5-amino-2-nitrobenzamide
show the reaction diagram
-
-
-
-
?
AGPYAHSS + H2O
?
show the reaction diagram
-
trypsin N143H/E151H hydrolyzes the peptide exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency
-
-
?
alpha-amylase + H2O
?
show the reaction diagram
-
-
-
?
alpha-casein + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-N-p-tosyl-L-arginine methyl ester + H2O
alpha-N-p-tosyl-L-arginine + methanol
show the reaction diagram
-
-
-
-
?
ANVIPFKVHFRAAFC + H2O
ANVIPFK + VHFR + AAFC
show the reaction diagram
-
-
-
-
?
apomyoglobin + H2O
?
show the reaction diagram
-
-
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
show the reaction diagram
-
-
?
benzoyl-Arg 4-nitroanilide + H2O
benzoyl-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
benzoyl-Arg ethyl ester + H2O
benzoyl-Arg + ethanol
show the reaction diagram
-
-
-
?
benzoyl-Arg ethyl ester + leucinamide
benzoyl-Arg leucinamide + ethanol
show the reaction diagram
-
-
-
?
benzoyl-Arg-4-nitroanilide + H2O
benzoyl-Arg + 4-nitroaniline
show the reaction diagram
-
-
?
benzoyl-Arg-p-nitroanilide + H2O
benzoyl-Arg + p-nitroaniline
show the reaction diagram
benzoyl-DL-Arg-4-nitroanilide + H2O
benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
benzoyl-DL-Arg-7-amido-4-methylcoumarin + H2O
benzoyl-DL-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzoyl-DL-Arg-p-nitroanilide + H2O
benzoyl-DL-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
benzoyl-DL-arginine-p-nitroanilide + H2O
benzoyl-DL-arginine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
benzoyl-L-Arg amide + H2O
?
show the reaction diagram
-
beta-trypsin is about 40% more active than alpha trypsin
-
-
?
benzoyl-L-Arg-4-nitroanilide + H2O
benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
benzoyl-L-arginine-p-nitroanilide + H2O
benzoyl-L-arginine + p-nitroaniline
show the reaction diagram
benzyloxycarbonyl-Arg-4-nitroanilide + H2O
benzyloxycarbonyl-Arg + 4-nitrolaniline
show the reaction diagram
-
-
?
benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O
?
show the reaction diagram
-
-
?
benzyloxycarbonyl-Arg-thiobenzyl ester + H2O
benzyloxycarbonyl-Arg + phenylmethanethiol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
94.8% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonyl-Glu-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Glu-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
75.2% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
14.8% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonyl-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-Pro-Arg-p-nitroanilide + H2O
benzyloxycarbonyl-Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-glutaminyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-glutamyl-L-lysyl-L-lysine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-glutamyl-L-lysyl-L-lysine + 7-amino-4-methylcoumarin
show the reaction diagram
-
low activity
-
-
?
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-leucyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-leucyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-phenylalaninyl-L-seryl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-valyl-L-leucyl-L-lysine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonyl-L-valyl-L-prolyl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
51.4% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
44.5% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
Benzyloxycarbonyl-Lys methyl ester + H2O
Benzyloxycarbonyl-Lys + methanol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Lys p-nitrophenyl ester + H2O
benzyloxycarbonyl-Lys + p-nitrophenol
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Lys-thiobenzyl ester + H2O
benzyloxycarbonyl-Lys + phenylmethanethiol
show the reaction diagram
benzyloxycarbonyl-Phe-Arg-4-nitroanilide + H2O
?
show the reaction diagram
-
-
?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
benzyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Val-Leu-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
27.1% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
33.5% of the activtiy with benzyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
-
?
benzyloxycarbonylglycyl-L-prolyl-L-arginine-4-methylcoumaryl-7-amide + H2O
benzyloxycarbonylglycyl-L-prolyl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
benzyloxycarbopnyl-L-Lys benzoyl ester + H2O
benzyloxycarbopnyl-L-Lys + benzoate
show the reaction diagram
-
-
-
-
?
beta-crystallin + H2O
?
show the reaction diagram
-
-
-
-
?
beta-lactoglobulin + H2O
?
show the reaction diagram
-
-
-
-
?
bovine heart cytochrome c + H2O
?
show the reaction diagram
-
-
-
-
?
bovine M-proinsulin + H2O
?
show the reaction diagram
-
insulin containing methionine at the N-terminus
cleaved at the K59R60-G61 bond of the C/A junction, but at the B/C junction cleavage occurs at the R31R32-E33 as well as the R31-R32E33 bond. Cleavage at the B/C junction is preferred (65%) over that at the C/A junction (35%)
-
?
Bovine serum albumin + H2O
?
show the reaction diagram
bovine serum albumin + H2O
LVVSTQTALA + ?
show the reaction diagram
-
-
-
-
?
bovine skin DQ-collagen type I + H2O
?
show the reaction diagram
calmodulin + H2O
?
show the reaction diagram
-
-
-
-
?
carbobenzoxy-L-Arg-7-amido-4-methylcoumarin + H2O
carbobenzoxy-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
carbonic anhydrase + H2O
?
show the reaction diagram
-
-
-
?
casein + H2O
?
show the reaction diagram
Cbz-L-Arg-7-amido-4-methylcoumarin + H2O
Cbz-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Collagen + H2O
?
show the reaction diagram
-
trypsin has little activity against collagen
-
-
?
Cry1Ab protoxin + H2O
Cry1Ab toxin
show the reaction diagram
-
-
-
-
?
Cytochrome c + H2O
?
show the reaction diagram
D-Phe-Pip-Arg 4-nitroanilide + H2O
D-Phe-Pip-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
D-Phe-piperidinyl-Arg-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
D-Val-Leu-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
D-Val-Leu-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
D-Val-Leu-Lys-thiobenzyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
DrmSP8-36 + H2O
?
show the reaction diagram
-
trypsin is the major endopeptidase regulating N-terminal truncated sex peptide DrmSP8-36 in hemolymph, the trypsin cleavage site R7-K8 is essential for release of the C-terminal fragment SP8-36 from sperm tail, the protein is cleaved more rapidly and completely in mated female hemolymph
-
-
?
enhanced green fluorescent protein-T1 + H2O
?
show the reaction diagram
-
enhanced green fluorescent protein-T1 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 20 and 8 kDa
-
-
?
enhanced green fluorescent protein-T1nb + H2O
?
show the reaction diagram
-
-
-
-
?
enhanced green fluorescent protein-T2 + H2O
?
show the reaction diagram
-
enhanced green fluorescent protein-T2 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 18 and 10 kDa
-
-
?
epithelial sodium channel + H2O
?
show the reaction diagram
factor Xa + H2O
?
show the reaction diagram
-
-
-
-
?
Fibronectin + H2O
?
show the reaction diagram
-
-
-
-
?
FITC-casein + H2O
?
show the reaction diagram
-
fluorescence-labeled casein
-
-
?
gamma-crystallin + H2O
?
show the reaction diagram
-
-
-
-
?
GDDSTPSILPAPRGYPGQV + H2O
GDDSTPSILPAPR + GYPGQV
show the reaction diagram
-
-
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
GERGFFYTPKT + H2O
?
show the reaction diagram
-
insulin-derived peptide
cleavage at bond K-T
-
?
GERGFFYTPKTRR + H2O
?
show the reaction diagram
-
insulin-derived peptide
cleavage at bond K-T
-
?
Glu-Gly-Arg-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
glucose oxidase + H2O
?
show the reaction diagram
-
-
-
?
Gly-Pro-Arg-p-nitroanilide + H2O
Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
glycogen phosphorylase b + H2O
?
show the reaction diagram
-
-
-
?
GPNSKGRSLIGRLDTPYGGC + H2O
GPNSKGR + SLIGRLDTPYGGC
show the reaction diagram
-
-
-
-
?
Hemoglobin + H2O
?
show the reaction diagram
high pathogenic hemagglutinin + H2O
?
show the reaction diagram
-
-
-
-
?
human cartilage collagen type II + H2O
?
show the reaction diagram
human M-proinsulin + H2O
?
show the reaction diagram
-
insulin containing methionine at the N-terminus
cleavage at the R31R32-E33 and K64R65-G66 bonds, i.e. B/C and C/A junctions. Cleavage at the B/C junction is preferred (65%) over that at the C/A junction (35%)
-
?
human placenta DQ-collagen type IV + H2O
?
show the reaction diagram
insulin
?
show the reaction diagram
-
-
cleavage at bond K29-T30
-
?
insulin + H2O
?
show the reaction diagram
inter-alpha-trypsin inhibitor heavy chain H4 + H2O
?
show the reaction diagram
-
inter-alpha-trypsin inhibitor heavy chain H4 is also a marker of acute ischemic stroke
-
-
?
intestinal alkaline sphingomyelinase bound to mucosa + H2O
free intestinal alkaline sphingomyelinase
show the reaction diagram
L-Phe-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
L-Phe-L-Pro-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide + H2O
L-Phe-L-Val-L-Ile-L-Arg + 5-amino-2-nitrobenzamide
show the reaction diagram
-
-
-
-
?
L-Phe-L-Val-L-Ile-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide + H2O
L-Phe-L-Val-L-Ile-L-Lys + 5-amino-2-nitrobenzamide
show the reaction diagram
-
-
-
-
?
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Arg-amide + H2O
L-Phe-L-Val-L-Pro-L-Arg + 5-amino-2-nitrobenzamide
show the reaction diagram
-
-
-
-
?
L-Phe-L-Val-L-Pro-N-(3-carbamoyl-4-nitrophenyl)-L-Lys-amide + H2O
L-Phe-L-Val-L-Pro-L-Lys + 5-amino-2-nitrobenzamide
show the reaction diagram
-
-
-
-
?
L-Tat (47-58) + H2O
?
show the reaction diagram
-
trypsin significantly inactivates L-Tat (47-58)
-
-
?
low pathogenic hemagglutinin + H2O
?
show the reaction diagram
-
-
-
-
?
lubricin + H2O
?
show the reaction diagram
-
-
-
-
?
Lysozyme + H2O
?
show the reaction diagram
-
-
-
?
M-diarginyl insulin + H2O
?
show the reaction diagram
-
insulin containing methionine at the N-terminus
cleavage at bond K29-T30
-
?
M-insulin
?
show the reaction diagram
-
insulin containing methionine at the N-terminus
cleavage at bond K29-T30
-
?
MeOSuc-Ala-Ala-Ala-Arg 4-nitroanilide + H2O
MeOSuc-Ala-Ala-Ala-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
MeOSuc-Ala-Ala-Arg 4-nitroanilide + H2O
MeOSuc-Ala-Ala-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
MeOSuc-Ala-Arg 4-nitroanilide + H2O
MeOSuc-Ala-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Myoglobin + H2O
?
show the reaction diagram
-
-
-
?
N-(p-tosyl)-Gly-Pro-Arg 4-nitroanilide + H2O
N-(p-tosyl)-Gly-Pro-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-acetyl-GTNRSSKGRSLIGKVDGTSHVTGKGVT-amide + H2O
SLIGKVDGTSHVTGKGVT-amide + N-acetyl-GTNRSSKGR
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-DL-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
N-alpha-benzoyl-DL-arginine-4-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
N-alpha-benzoyl-DL-arginine-p-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + p-nitroaniline
show the reaction diagram
N-alpha-benzoyl-L-Arg ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
show the reaction diagram
N-alpha-benzoyl-L-Arg methyl ester + H2O
N-alpha-benzoyl-L-Arg + methanol
show the reaction diagram
N-alpha-benzoyl-L-Arg-2-naphthylamide + H2O
N-alpha-benzoyl-L-Arg + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
N-alpha-benzoyl-L-Arg-ethyl ester + H2O
N-alpha-benzoyl-L-Arg + ethanol
show the reaction diagram
N-alpha-benzoyl-L-arginine 4-nitroanilide + H2O
N-alpha-benzoyl-L-arginine + 4-nitroanilide
show the reaction diagram
-
-
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
?
show the reaction diagram
N-alpha-benzoyl-L-arginine ethyl ester + H2O
N-alpha-benzoyl-L-arginine + ethanol
show the reaction diagram
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O
N-alpha-benzoyl-L-arginine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Lys-p-nitroanilide + H2O
N-alpha-benzoyl-L-Lys + p-nitroaniline
show the reaction diagram
N-alpha-benzoyl-L-Phe-L-Val-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Phe-L-Val-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O
N-alpha-benzoyl-L-Pro-L-Phe-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-alpha-p-tosyl-L-Arg methyl ester + H2O
N-alpha-p-tosyl-L-Arg + methanol
show the reaction diagram
-
-
-
-
?
N-alpha-p-tosyl-L-arginine methyl ester + H2O
N-alpha-p-tosyl-L-arginine + ?
show the reaction diagram
-
-
-
-
?
N-alpha-p-tosyl-L-arginine methyl ester + H2O
N-alpha-p-tosyl-L-arginine + ethanol
show the reaction diagram
-
-
-
-
?
N-alpha-p-tosyl-L-arginine methyl ester + H2O
N-alpha-p-tosyl-L-arginine + methanol
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-Arg-4-nitroanilide + H2O
N-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-Arg-4-nitroanilide + H2O
N-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-DL-arginine 4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
N-benzoyl-DL-arginine-4-nitroanilide + H2O
N-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
?
N-benzoyl-DL-arginyl-4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
?
N-Benzoyl-L-Arg ethyl ester + H2O
?
show the reaction diagram
N-benzoyl-L-Arg-4-nitroanilide + H2O
N-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-arginine 4-nitroanilide + H2O
N-benzoyl-L-arginine + 4-nitroaniline
show the reaction diagram
-
the trypsin-like proteinases from the resistant strains exhibited higher affinity for the substrate than the enzymes from the susceptible strains
-
-
?
N-benzoyl-L-arginine ethyl ester + H2O
N-benzoyl-L-arginine + ethanol
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-arginine-p-nitroanilide + H2O
N-benzoyl-L-arginine + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine methyl ester + H2O
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine + methanol
show the reaction diagram
-
-
-
-
?
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide + H2O
N-benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Gly-L-Pro-L-Arg-4-nitroanilide + H2O
N-benzyloxycarbonyl-Gly-L-Pro-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Gly-Pro-Arg 4-nitroanilide + H2O
N-benzyloxycarbonyl-Gly-Pro-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
N-carbobenzyloxy-Gly-Pro-Arg-p-nitroanilide + H2O
N-carbobenzyloxy-Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
Nafamostat + H2O
?
show the reaction diagram
-
extremely poor substrate
-
?
Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine-4-nitroanilide + H2O
Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-4-tosyl-L-Arg methyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-4-tosyl-L-arginine methyl ester + H2O
Nalpha-4-tosyl-L-arginine + methanol
show the reaction diagram
Nalpha-benzoyl-DL-Arg 2-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha-Benzoyl-DL-Arg 4-nitroanilide + H2O
Nalpha-Benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-Arg-p-nitroanilide + H2O
?
show the reaction diagram
Nalpha-benzoyl-DL-arginine 4-nitroanilide + H2O
Nalpha-benzoyl-DL-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-arginine 4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-L-Arg-4-nitroanilide + H2O
Nalpha-benzoyl-L-Arg + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
Nalpha-benzoyl-L-arginine + ethanol
show the reaction diagram
Nalpha-Boc-D-Ala-(p-amidino)phenyl ester + D-Ala-4-nitroanilide
Nalpha-Boc-D-Ala-D-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Ala-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Ala-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Ala-Gly-(4-carbamido)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Ala-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Leu-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Leu-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Leu-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Leu-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Phe-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Phe-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-D-Phe-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-D-Phe-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-Gly-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-Gly-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-Gly-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-Gly-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Ala-(p-amidino)phenyl ester + D-Ala-4-nitroanilide
Nalpha-Boc-L-Ala-D-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Ala-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Ala-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Ala-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Ala-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Leu-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Leu-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Leu-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Leu-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Phe-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Phe-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nalpha-Boc-L-Phe-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nalpha-Boc-L-Phe-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nalpha-p-tosyl-L-Arg methyl ester + H2O
?
show the reaction diagram
Nalpha-p-tosyl-L-arginine methyl ester + H2O
?
show the reaction diagram
NATLDPRSFLLRNPNDKYE + H2O
NATLDPR + SFLLRNPNDKYE
show the reaction diagram
-
-
-
-
?
Nbeta-Boc-beta-Ala-(p-amidino)phenyl ester + L-Ala-4-nitroanilide
Nbeta-Boc-beta-Ala-L-Ala-4-nitroanilide + 4-hydroxybenzimidamide
show the reaction diagram
-
-
-
-
?
Nbeta-Boc-beta-Ala-(p-guanidino)phenyl ester + beta-Ala-4-nitroanilide
Nbeta-Boc-beta-Ala-beta-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
Nbeta-Boc-beta-Ala-(p-guanidino)phenyl ester + L-Ala-4-nitroanilide
Nbeta-Boc-beta-Ala-L-Ala-4-nitroanilide + 1-(4-hydroxyphenyl)guanidine
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AAGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AAGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AAGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-ADGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ADGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-ADGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AEGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AEGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AEGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AFGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AFGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AFGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AGARGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGAR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGARGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AGDRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGDR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGDRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AGERGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGER + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGERGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AGFRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGFR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGFRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AGGKGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AGGRAAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRDAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GDGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGREAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GEGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRFAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GFGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GGGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AGGRGDGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGEGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGFGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGGGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGHGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGIGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGKGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGLGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGMGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGNGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGQGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGSGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGTGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGVGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRGYGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGGRHAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GHGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRIAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GIGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRKAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GKGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRLAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GLGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRMAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GMGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRNAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GNGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRQAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GQGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRSAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GSGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRTAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GTGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRVAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GVGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGGRYAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGGR + GYGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGHRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGHR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGHRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGIRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGIR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGIRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AGKRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGKR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGKRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AGLRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGLR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGLRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGMRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGMR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGMRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGNRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGNR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGNRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGQRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGQR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGQRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AGSRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGSR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGSRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AGTRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGTR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGTRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AGVRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGVR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGVRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AGYRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AGYR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AGYRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AHGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AHGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AHGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AIGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AIGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AIGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AKGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AKGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AKGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-ALGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ALGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-ALGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AMGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AMGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AMGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-ANGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ANGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-ANGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AQGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AQGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AQGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-ASGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ASGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-ASGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-ATGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-ATGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-ATGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-AVGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AVGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AVGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
o-aminobenzoyl-AYGRGAGQ-(2,3-dinitrophenyl-ethylene diamine) + H2O
o-aminobenzoyl-AYGR + GAGQ-(2,3-dinitrophenyl-ethylene diamine)
show the reaction diagram
-
-
-
?
o-aminobenzoyl-AYGRGAGQ-(2,4-dinitrophenyl-ethylene diamine) + H2O
?
show the reaction diagram
ovalbumin + H2O
?
show the reaction diagram
-
-
-
?
p-amidinophenyl acetate + H2O
p-amidinophenol + acetate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl p'-guanidinobenzoate + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-p'-guanidino benzoate + H2O
p-nitrophenol + p-guanidinobenzoate
show the reaction diagram
p-tosyl-L-Arg methyl ester + H2O
?
show the reaction diagram
Pefachrome + H2O
?
show the reaction diagram
-
-
-
?
Pefachrome tPA + H2O
?
show the reaction diagram
-
-
-
?
peroxidase + H2O
?
show the reaction diagram
-
-
-
?
pre-Pro-Arg-insulin + H2O
mono/di-Arg insulin + ?
show the reaction diagram
-
-
-
-
-
Pro-Arg-insulin + H2O
mono/di-Arg insulin + ?
show the reaction diagram
-
-
-
-
-
pro-human defensin-5 + H2O
human defensin-5 + pro-human defensin-5 propeptide
show the reaction diagram
-
pro-human defensin-5 in the intestinal lumen is processed by trypsin in a complex in which chymotrypsinogen is also cleaved for activation
-
-
?
pro-insulin + H2O
human insulin-threonine-ester
show the reaction diagram
-
-
-
-
-
pro-matrix metalloproteinase-2 + H2O
matrix metalloproteinase-2 + pro-peptide
show the reaction diagram
-
activation by trypsin depends on various factors such as the level of exogenously added Ca2+ and Brij-35, temperature, and trypsin concentration. Activation occurs as sequential processing, initially generating an active 62 kDa species followed by successive truncation of the C-terminal domain leading to active species of 56 kDa, 52 kDa and 50kDa. Comparison with activation of pro-matrix metalloproteinase-2 by membrane-type 1 metalloproteinase or 4-aminophenylmercuric acetate
-
-
?
protease-activated receptor-1 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-2 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-3 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-4 + H2O
?
show the reaction diagram
-
activation
-
-
?
proteinase-activated receptor 1 + H2O
?
show the reaction diagram
-
-
-
-
?
proteinase-activated receptor PAR-1 + H2O
activated proteinase-activated receptor PAR-1
show the reaction diagram
-
no activation by mesotrypsin in epithelial cells, but mesotrypsin activates PAR-1 in astrocytoma cells. Half-maximal response for both cationic and anionic trypsin at about 10-20 nM
-
-
?
proteinase-activated receptor PAR-2 + H2O
activated proteinase-activated receptor PAR-2
show the reaction diagram
-
no activation by mesotrypsin in epithelial cells. Half-maximal response for both cationic and anionic trypsin at about 5 nM
-
-
?
proteinase-activated receptor PAR-3 + H2O
activated proteinase-activated receptor PAR-3
show the reaction diagram
-
no activation by mesotrypsin in epithelial cells. Half-maximal response for both cationic and anionic trypsin at about 10-20 nM
-
-
?
S-2222 + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-Ala-Ala-Pro-Arg-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Leu + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-Ala-Ala-Pro-Lys-p-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
?
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Trp + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin + H2O
succinyl-Ala-Ala-Pro-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
succinyl-L-Ala-L-Ala-L-Pro-L-Leu-7-amido-4-methylcoumarin + H2O
succinyl-L-Ala-L-Ala-L-Pro-L-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
superoxide dismutase 1 + H2O
?
show the reaction diagram
-
-
-
-
?
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin + H2O
tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
tert-butyloxycarbonyl-Gln-Ala-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
tert-butyloxycarbonyl-Gln-Arg-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
tert-butyloxycarbonyl-Gln-Gly-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
?
Tos-L-Arg-OMe + H2O
?
show the reaction diagram
tosyl-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
?
show the reaction diagram
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
?
tosyl-Gly-Pro-Lys-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
tosyl-Gly-Pro-Lys-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
?
tosyl-GPK-p-nitroanilide + H2O
tosyl-GPK + p-nitroaniline
show the reaction diagram
tosyl-GPR-p-nitroanilide + H2O
tosyl-GPR + p-nitroaniline
show the reaction diagram
ubiquitin + H2O
?
show the reaction diagram
-
-
-
-
?
VP4 + H2O
fragments VP8* and VP5*
show the reaction diagram
-
outer layer protein of rotavirus capsid
-
-
?
Z-Ala-Arg-Arg-7-amido-4-methylcoumarin + H2O
Z-Ala-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low amidolytic activity
-
-
?
Z-Arg-7-amido-4-methylcoumarin + H2O
Z-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Z-Arg-Arg-7-amido-4-methylcoumarin + H2O
Z-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low amidolytic activity
-
-
?
Z-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
Z-Gly-Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low amidolytic activity
-
-
?
Z-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
Z-Gly-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Z-Gly-Pro-Arg-p-nitroanilide + H2O
Z-Gly-Pro-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Z-Phe-Arg-4-methylcoumaryl-7-amide + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low activity
-
-
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
Z-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
high amidolytic activity
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DrmSP8-36 + H2O
?
show the reaction diagram
-
trypsin is the major endopeptidase regulating N-terminal truncated sex peptide DrmSP8-36 in hemolymph, the trypsin cleavage site R7-K8 is essential for release of the C-terminal fragment SP8-36 from sperm tail, the protein is cleaved more rapidly and completely in mated female hemolymph
-
-
?
enhanced green fluorescent protein-T1 + H2O
?
show the reaction diagram
-
enhanced green fluorescent protein-T1 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 20 and 8 kDa
-
-
?
enhanced green fluorescent protein-T2 + H2O
?
show the reaction diagram
-
enhanced green fluorescent protein-T2 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 18 and 10 kDa
-
-
?
Gelatin + H2O
?
show the reaction diagram
-
-
-
-
?
human cartilage collagen type II + H2O
?
show the reaction diagram
-
use of enzyme and its regulators as markers of prognosis and disease activity in rheumatoid arthritis
-
-
?
intestinal alkaline sphingomyelinase bound to mucosa + H2O
free intestinal alkaline sphingomyelinase
show the reaction diagram
-
cleavage at R440, first tryptic site upstream of signal anchor. Physiological role of enzyme in sphingomyelin digestion
-
-
?
protease-activated receptor-1 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-2 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-3 + H2O
?
show the reaction diagram
-
activation
-
-
?
protease-activated receptor-4 + H2O
?
show the reaction diagram
-
activation
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Na+
-
Na+ activation is interwoven with substrate selectivity in the trypsin scaffold
Ni2+
-
trypsin N143H/E151H hydrolyzes the peptide AGPYAHSS exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency
Zn2+
-
trypsin N143H/E151H hydrolyzes the peptide AGPYAHSS exclusively in presence of Ni2+ or Zn2+ with high levels of catalytic efficiency
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2R,4R)-4-phenyl-1-[Nalpha-(7-methoxy-2-naphthalenesulfonyl)-L-arginyl]-2-piperidinecarboxylic acid
-
competitive, small molecular weight inhibitor without tryptase inhibitor activity
(4-(6-chloro-naphthalene-2-sulfonyl)-piperazin-1-yl)-(3,4,5-6-tetrahydro[2H-1,4']bipyridinyl-4yl)-methanone
-
-
1,10-phenanthroline
-
-
1-(L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane
1-[N2-[(7-methoxynaphthalen-2-yl)sulfonyl]-L-arginyl]-4-phenylpiperidine-2-carboxylic acid
-
IC50: 0.0003 mM
2,7-bis(4-amidinobenzylidene)-cycloheptan-1-one
-
-
2,7-bis(4-amidinobenzylidene)cycloheptan-1-one
-
unspecific strong inhibition
2-mercaptoethanol
-
0.1 mM, isoenzyme aT-I loses 90% of its activity, isoenzyme aT-II loses 93% of its activity
3,4-dichloroisocoumarin
-
-
4-(2-aminoethyl)-benzensulfonylfluoride
-
isozyme TryIII activity is completely (more than 95%) inhibited by 4-(2-aminoethyl)-benzensulfonylfluoride
4-(amidinophenyl)methanesulfonyl fluoride
-
-
6-amidino-2-naphthol
-
mixed-type inhibition
acetonitrile
-
partial inhibition at 20% acetonitrile, a 5% aqueous acetonitrile solution less likely reduces the activity of the porous polymer monolith-immobilized trypsin
acyclic SFTI
-
i.e. oSFTI, modified sunflower trypsin inhibitor 1, SFTI-1, generated by Fmoc-based automated synthesis
acyclic sun flower trypsin inhibitor-1
-
-
-
Adenanthera pavonina trypsin inhibitor
Adunil CEL
-
0.1% v/v, 47.3% residual activity
-
alpha-1-antitrypsin
-
in gestational diabetes mellitus reduction of serum trypsin inhibitory capacity may be due to non-enzymatic glycosylation of alpha-1-antitrypsin or oxidation of methionine in the active site of alpha-1-antitrypsin
-
Alpha1-antitrypsin
-
-
-
alpha1-antitrypsin Pittsburgh
-
effective inhibitor, variant of alpha1-antitrypsin containing an Arg residue in place of the P1 Met358 in the reactive-site peptide bond
-
antipain
Apios Americana trypsin inhibitor
-
AATI, strong inhibitor, complete inhibition with Apios Americana trypsin inhibitor bound to trypsin in a 1:2 molar ratio
-
Aprotinin
atropine
-
strongly diminishes trypsin activity in stressed animals
AVNIPFKVHFRCKAAFC
-
a small trypsin inhibitor from the skin secretion of the frog Odorrana grahami, only inhibits the hydrolysis activity of trypsin on synthetic chromogenic substrate
Bauhinia variegata var. variegata trypsin inhibitor
-
i.e. BvvTI, a Kunitz-type inhibitor from seeds of the Camel’s foot tree, Bauhinia variegata var. variegata. The inhibitor is also capable of significant inhibition of the proliferation of nasopharyngeal cancer CNE-1 cells in a selective way and of inhibiting anti-HIV-1 reverse transcriptase activity, overview
-
benzamidine
benzylamine
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-ethylphenyl)phosphonate
-
50% inhibition at 0.000017 mM, comparison with inhibitory effect on urokinase
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-isopropylphenyl)phosphonate
-
50% inhibition at 0.000061 mM, comparison with inhibitory effect on urokinase
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-methylthiophenyl)phosphonate
-
50% inhibition at 0.0000087 mM, comparison with inhibitory effect on urokinase
benzyloxycarbonyl-amino(4-guanidinophenyl)methyl-bis(4-t-butylphenyl)phosphonate
-
50% inhibition at 0.000088 mM, comparison with inhibitory effect on urokinase
bis(m-amidinosalicylidene-L-alaninato)iron(III)
-
-
bis(p-amidinosalicylidene-L-alaninato)iron(III)
-
-
-
bisphenol A
-
the secondary and tertiary structures of trypsin are altered by bisphenol S binding, which results in the loosening of the skeleton of the enzyme. Bisphenol S induces microenvironmental changes around tyrosine and tryptophan residues of trypsin. The activity of trypsin does not change remarkably with the increasing concentration of bisphenol S. The binding of bisphenol S to trypsin is a spontaneous process and hydrogen bonding and hydrophobic interactions play a vital role in stabilizing the bisphenol S-trypsin complex. The binding constants of bisphenol S with trypsin are 74200 (25°C) and 59100 L/mol (37°C)
black gram trypsin inhibitor 1
-
highly effective trypsin inhibitor, 87.5% of trypsin inhibitory activity of black gram trypsin inhibitor 1 is retained after treatment with 10 mM dithiothreitol for 2 hours, and the activity dwindles to 12.5% after treatment with 100 mM dithiothreitol for 2 hours
-
black gram trypsin inhibitor 2
-
highly effective trypsin inhibitor, the trypsin inhibitory activity of black gram trypsin inhibitor 2 is unaffected after exposure to 100 mM dithiothreitol for 2 hours
-
black gram trypsin inhibitor 3
-
highly effective trypsin inhibitor, in the presence of 10 mM dithiothreitol trypsin inhibitory of black gram trypsin inhibitor 3 drops to 57.1% after 1 h and becomes undetectable after 2 hours, while treatment of 100 mM dithiothreitol for 1 h has the same effect
-
Bovine pancreatic trypsin inhibitor
bovine secretory inhibitors
-
A and B
-
Bowman-Birk inhibitor
BPTI-20st
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simplified variant of bovine pancreatic trypsin inhibitor containing 20 alanines, retains a wild type level of trypsin inhibitory activity
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BPTI-[5,55]st
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single-disulfide-bonded variant of bovine pancreatic trypsin inhibitor, retains a wild type level of trypsin inhibitory activity
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captopril
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feeding angiotensin converting enzyme inhibitor captopril down-regulates trypsin activity in the larval gut, injecting third instar larvae with a combination of Aedes aegypti trypsin-modulating oostatic factor and captopril downregulates trypsin biosynthesis in the larval gut
Cd2+
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10 mM, 48% inhibition
chymostatin
clispin
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a Clitocybe nebularis trypsin inhibitor variant, recombinantly expressed in Escherichia coli, highly specific towards trypsin, fast-acting, and tight-binding. Molecular cloning of the gene and cDNA encoding cnispin, overview
Clitocybe nebularis trypsin inhibitor
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CnSPI, several inhibitor variants, separation by trypsin affinity chromatography. Possible regulatory role for CnSPIs in the endogenous proteolytic system of Clitocybe nebularis. Isolation from frozen fruiting bodies or basidiocarps
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cMCoTI-II
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-
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cyclic SFTI
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i.e. cSFTI, modified sunflower trypsin inhibitor 1, SFTI-1, generated by Fmoc-based automated synthesis
dithiothreitol
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1 mM, no residual activity
DMTI-II
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trypsin inhibitor from seeds of Dimorphandra mollis. The inhibitory activity is stable over a wide range and in the presence of DTT
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domesticated soybean inhibitor
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SBTI
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DOTA-SFTI
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modified sunflower trypsin inhibitor 1, SFTI-1, labeled, generated by Fmoc-based automated synthesis
E64
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weak inhibition
Ecballium elaterium trypsin inhibitor II
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EETI, a a model member of the knottin family, used for construction of libraries of EETI loop-substituted variants with diversity in both amino acid sequence and loop length, wild-type EETI or loop-substituted clones expressed from yeast, structure-function analysis, modelling, overview
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enalapril
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feeding angiotensin converting enzyme inhibitor enalapril down-regulates trypsin activity in the larval gut
ethylenediaminetetraacetic acid
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partial
Gly-L-Arg-(2-amino butanoyl)-L-Thr-Nphe-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-(2-amino butanoyl)-L-Phe-L-Pro-L-Asp
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Gly-L-Arg-L-Cys-L-Thr-L-Lys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp
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sun flower trypsin inhibitor SFTI-1
Glycine max trypsin inhibitor
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from raw soymilk, inhibition kinetics, overview
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I-ovalbumin
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high affinity interaction of enzyme with I-ovalbumin, the product of a heating transition of ovalbumin which acts as a potent reversible serine proteinase inhibitor. Interaction is characterized by high kinetic association constants and low kinetic dissociation konstants
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iodoacetic acid
K+
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10 mM, 16% inhibition
Kunitz domain 1 of tissue factor pathway inhibitor-2
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-
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Kunitz type trypsin inhibitor from Glycine max
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isolated and purified from Korean large black soybeans cultivar, overview. Inhibits bovine trypsin, but also shows Anti-HIV reverse transcriptase activity, cytokine-inducing activity and antiproliferative activity on tumor cell lines
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L-1-chloro-3-tosylamido-4-phenyl-2-butanone
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L-1-chloro-3-[4-tosylamido]-4-phenyl-2-butanone
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L-Lys-L-Cys-L-Thr-Nlys-L-Ser-L-Ile-L-Pro-L-Pro-L-Ile-L-Cys-L-Phe-L-Pro-L-Asp
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leech-derived tryptase inhibitor
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i.e. LDTI, purely competitive inhibitor
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LEKTI
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the LEKI gene encodes a 15-domain serine proteinase inhibitor, disulfide bonds are important for LEKTI function, noncompetitive mechanism, expression and purification of human LEKTI gene in Sf9
Leupeptin
Li+
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10 mM, 19% inhibition
lily bulb trypsin inhibitor
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-
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Lima bean trypsin inhibitor
LKGCWTKSIPPKPCFGK
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Locusta migratoria protease inhibitor 1
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strong inhibition
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Locusta migratoria protease inhibitor 3
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strong inhibition, the inhibitor interacts with trypsin through the reactive site P3-P4' and the P10-P6 residues
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low-molecular-mass trypsin inhibitor inhibitor type-2
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from Sinapis alba, inhibitor consists of a peptide mixture, displaying Ile or Arg at position 43, Trp or kynurenine at position 44, and C-terminal ragged ends
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m-amidinosalicylidene-L-alaninato(aqua)copper(II)
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m-amidinosalicylidene-L-alaninato(aqua)copper(II) hydrochloride
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-
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m-guanidinosalicylidene-L-alaninato(aqua)copper(II)
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mung bean trypsin inhibitor
mustard trypsin inhibitor 2
mustard trypsin inhibitor II
inhibition of HzTrypsin-C, no inhibition of HzTrypsin-S
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N-alpha-p-tosyl-L-lysine chloromethyl ketone
N-alpha-tosyl-L-Lys-chloromethyl ketone
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complete inhibition at 1 mM
N-alpha-tosyl-L-lysine chlormethylketone
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less than 50% inhibition of isozyme TryIII by the trypsin-specific inhibitor N-alpha-tosyl-L-lysine chlormethylketone
N-alpha-tosyl-L-lysine chloromethyl ketone
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N-alpha-tosyl-L-lysine-chloromethylketone
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complete inhibition at 5 mM
N-p-tosyl-L-lysine chloromethyl ketone
N-p-tosyl-lysine chloroketone
N-tosyl-L-lysine chloromethyl ketone
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82.9% inhibition at 5 mM
N-tosyl-L-lysine-chloromethyl ketone
N-tosyl-L-phenyl-alanine-chloromethyl ketone
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11% inhibition at 1mg/ml
N-tosyl-L-phenylalanine-chloromethyl ketone
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5.34% inhibition at 5 mM
Na+
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10 mM, 21% inhibition
Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride
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inhibits trypsin specifically and irreversibly, 40% reduced activity in the presence of 1 mM Na-p-tosyl-L-lysine chloromethyl ketone hydrochloride
Nafamostat
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potent inhibitor
nafamostat mesilate
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IC50: 0.00003 mM
Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-4'-acetyl-piperazide
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Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-4'-methylpiperidide
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Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-D-pipecolic acid
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Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-D-pipecolic acid methyl ester
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Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-isopecotinic acid
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Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-isopecotinic acid methylester
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Nalpha-(2-naphthylsulfonyl)-L-3-amidino-phenylalanine-piperazide
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Nalpha-p-tosyl-L-lysine chloromethyl ketone
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complete inhibition
Nalpha-tosyl-L-Lys-chloromethyl ketone
Nalpha-tosyl-L-Lys-chloromethylketone
Nalpha-tosylglycyl-3-amidino-DL-phenylalanine-methyl ester
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napsagatran
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napsagatran ethylester
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nexin-1
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nexin-1 inhibits isozymes trypsin-1 ad trypsin-2, nexin-1 inhibits trypsin-4 and forms stable complexes only with this trypsin isoenzyme
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oMCoTI-II
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-
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ovomucoid
p-amidinosalicylidene-L-alaninato(aqua)copper(II)
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p-nitrophenyl-p'-guanidino-benzoate
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irreversible trypsin inhibitor
p-tosyl-L-Lys-chloromethane
pancreatic secretory trypsin inhibitor
Pancreatic trypsin inhibitor
PdKI-3.1
PdKI-3.2
pefabloc
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Pefabloc SC
Pepstatin
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pepstatin A
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26% inhibition at 0.01 mM
Phaseolus vulgaris trypsin inhibitor
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two different variants of 132 kDa and 118 kDA from navy beans and red kidney beans, respectively, isolated by heat treatment and ammonium sulfate fractionation
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phenyl methyl sulphonyl fluoride
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100% inhibition
phenylbutylamine
Phenylethylamine
phenylmethyl sulfonyl fluoride
phenylmethylsulfonyl fluoride