Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli BL21-CodonPlus DE3-RIL cells | Aeropyrum pernix K1 |
Protein Variants | Comment | Organism |
---|---|---|
E88A | mutant, lower thermodynamic stability than the wild-type, broken inter-domain salt bridge, catalytic activity almost abolished | Aeropyrum pernix K1 |
E88A/R526E | mutant, lower thermodynamic stability than the wild-type | Aeropyrum pernix K1 |
E88A/R526K | mutant, lower thermodynamic stability than the wild-type, broken inter-domain salt bridge, positive charge at position 526, catalytic activity almost abolished | Aeropyrum pernix K1 |
E88A/R526V | mutant, lower thermodynamic stability than the wild-type | Aeropyrum pernix K1 |
E88D | mutant, lower thermodynamic stability than the wild-type | Aeropyrum pernix K1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.467 | - |
Ac-Leu-p-nitroanilide | mutant R526V, in the presence of 1 M sodium chloride | Aeropyrum pernix K1 | |
0.49 | - |
Ac-Leu-p-nitroanilide | wild-type | Aeropyrum pernix K1 | |
0.695 | - |
Ac-Leu-p-nitroanilide | mutant E88A/R526V, in the presence of 1 M sodium chloride | Aeropyrum pernix K1 | |
0.748 | - |
Ac-Leu-p-nitroanilide | wild-type, in the presence of 1 M sodium chloride | Aeropyrum pernix K1 | |
1.14 | - |
Ac-Leu-p-nitroanilide | mutant E88A | Aeropyrum pernix K1 | |
1.19 | - |
Ac-Leu-p-nitroanilide | mutant E88A/R526V | Aeropyrum pernix K1 | |
1.76 | - |
Ac-Leu-p-nitroanilide | mutant E88A, in the presence of 1 M sodium chloride | Aeropyrum pernix K1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aeropyrum pernix K1 | removal of an N-acylated amino acid from blocked peptides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix K1 | Q9YBQ2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aeropyrum pernix K1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-Leu-p-nitroanilide + H2O | substrate peptidase assay | Aeropyrum pernix K1 | Ac-Leu + p-nitroaniline | - |
? | |
additional information | removal of an N-acylated amino acid from blocked peptides | Aeropyrum pernix K1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acylpeptide hydrolase | - |
Aeropyrum pernix K1 |
apAPH | - |
Aeropyrum pernix K1 |
APH | - |
Aeropyrum pernix K1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
activity assay | Aeropyrum pernix K1 |
90 | - |
- |
Aeropyrum pernix K1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
Ac-Leu-p-nitroanilide | mutant E88A | Aeropyrum pernix K1 | |
8.75 | - |
Ac-Leu-p-nitroanilide | mutant E88A/R526V | Aeropyrum pernix K1 | |
10.5 | - |
Ac-Leu-p-nitroanilide | mutant R526V, in the presence of 1 M sodium chloride | Aeropyrum pernix K1 | |
10.6 | - |
Ac-Leu-p-nitroanilide | wild-type | Aeropyrum pernix K1 | |
10.9 | - |
Ac-Leu-p-nitroanilide | mutant E88A/R526V, in the presence of 1 M sodium chloride | Aeropyrum pernix K1 | |
32.4 | - |
Ac-Leu-p-nitroanilide | mutant E88A, in the presence of 1 M sodium chloride | Aeropyrum pernix K1 | |
36.4 | - |
Ac-Leu-p-nitroanilide | wild-type, in the presence of 1 M sodium chloride | Aeropyrum pernix K1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Aeropyrum pernix K1 |