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Literature summary for 3.4.19.1 extracted from

  • Szeltner, Z.; Kiss, A.L.; Domokos, K.; Harmat, V.; Naray-Szabo, G.; Polgar, L.
    Characterization of a novel acylaminoacyl peptidase with hexameric structure and endopeptidase activity (2009), Biochim. Biophys. Acta, 1794, 1204-1210.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pET15b for expression in Escherichia coli Rosetta DE3 cells Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73230
-
calculated, monomer Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pyrococcus horikoshii cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide ?
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58323
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-EFSPF(NO2)RA + H2O
-
Pyrococcus horikoshii ?
-
?
Abz-GFEPF(NO2)RA + H2O
-
Pyrococcus horikoshii ?
-
?
Abz-KARVLF(NO2)EANle + H2O
-
Pyrococcus horikoshii ?
-
?
Abz-SAVLQSGF(NO2)A + H2O
-
Pyrococcus horikoshii ?
-
?
Ac-Ala-7-amido-4-methylcoumarin + H2O
-
Pyrococcus horikoshii N-acetyl-L-Ala + 7-amino-4-methylcoumarin
-
?
Ac-Leu-4-nitroanilide + H2O
-
Pyrococcus horikoshii N-acetyl-L-Leu + 4-nitroaniline
-
?
Ac-Phe-2-naphthylamide + H2O
-
Pyrococcus horikoshii ?
-
?
Ac-Phe-2-naphthylamide + H2O
-
Pyrococcus horikoshii N-acetyl-L-Phe + 2-naphthylamine
-
?
Ac-Phe-4-nitroanilide + H2O
-
Pyrococcus horikoshii N-acetyl-L-Phe + 4-nitroaniline
-
?
Gly-Phe-2-naphthylamide + H2O
-
Pyrococcus horikoshii Gly-Phe + 2-naphthylamine
-
?
additional information cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide Pyrococcus horikoshii ?
-
?

Subunits

Subunits Comment Organism
hexamer
-
Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
AAP
-
Pyrococcus horikoshii
acylaminoacyl peptidase
-
Pyrococcus horikoshii
PhAAP
-
Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
kinetic assay Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
kinetic assay Pyrococcus horikoshii