Cloned (Comment) | Organism |
---|---|
plasmid containing full-length acylpeptide hydrolase used as template to generate pFLAG-CMV1 expression construct. This construct encodes amino acids 2-732 of acylpeptide hydrolase. For protein production, transfection into 293 cells | synthetic construct |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ac-Gly-prolineboronic acid | - |
synthetic construct | |
acetyl-Gly-prolineboronic acid | selectivity for APH 25fold higher than for fibroblast activation protein | synthetic construct |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
synthetic construct | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by affinity chromatography, more than 95% pure | synthetic construct |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Ala-7-amido-4-methylcoumarin + H2O | - |
synthetic construct | acetyl-Ala + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acylpeptide hydrolase | - |
synthetic construct |
APH | - |
synthetic construct |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000575 | - |
Ac-Gly-prolineboronic acid | - |
synthetic construct |