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Literature summary for 3.4.19.1 extracted from

  • Edosada, C.Y.; Quan, C.; Wiesmann, C.; Tran, T.; Sutherlin, D.; Reynolds, M.; Elliott, J.M.; Raab, H.; Fairbrother, W.; Wolf, B.B.
    Selective inhibition of fibroblast activation protein protease based on dipeptide substrate specificity (2006), J. Biol. Chem., 281, 7437-7444.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
plasmid containing full-length acylpeptide hydrolase used as template to generate pFLAG-CMV1 expression construct. This construct encodes amino acids 2-732 of acylpeptide hydrolase. For protein production, transfection into 293 cells synthetic construct

Inhibitors

Inhibitors Comment Organism Structure
Ac-Gly-prolineboronic acid
-
synthetic construct
acetyl-Gly-prolineboronic acid selectivity for APH 25fold higher than for fibroblast activation protein synthetic construct

Organism

Organism UniProt Comment Textmining
synthetic construct
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography, more than 95% pure synthetic construct

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Ala-7-amido-4-methylcoumarin + H2O
-
synthetic construct acetyl-Ala + 7-amino-4-methylcoumarin
-
?

Synonyms

Synonyms Comment Organism
acylpeptide hydrolase
-
synthetic construct
APH
-
synthetic construct

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000575
-
Ac-Gly-prolineboronic acid
-
synthetic construct