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Literature summary for 3.4.19.1 extracted from

  • Yamauchi, Y.; Ejiri, Y.; Toyoda, Y.; Tanaka, K.
    Identification and biochemical characterization of plant acylamino acid-releasing enzyme (2003), J. Biochem., 134, 251-257.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a functional enzyme Cucumis sativus
expression in Escherichia coli as a functional enzyme Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, complete inhibition of recombinant enzyme Arabidopsis thaliana
Cu2+ 1 mM, complete inhibition of recombinant enzyme Cucumis sativus
diisopropyl fluorophosphate 1 mM, complete inactivation Arabidopsis thaliana
diisopropyl fluorophosphate 1 mM, complete inactivation Cucumis sativus
NEM 1 mM Arabidopsis thaliana
NEM 1 mM Cucumis sativus
PCMB 0.1 mM Arabidopsis thaliana
PCMB 0.1 mM Cucumis sativus
PMSF 1 mM, 50% inhibition Arabidopsis thaliana
PMSF 1 mM, 50% inhibition Cucumis sativus
Zn2+ 1 mM, complete inhibition of recombinant enzyme Arabidopsis thaliana
Zn2+ 1 mM, complete inhibition of recombinant enzyme Cucumis sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
N-acetyl-Ala-p-nitroanilide pH 7.0, 37°C Arabidopsis thaliana
4.6
-
N-acetyl-Ala-p-nitroanilide pH 7.0, 37°C Cucumis sativus

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast stroma
-
Cucumis sativus 9570
-
chloroplast stroma
-
Arabidopsis thaliana 9570
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
4 * 82000, SDS-PAGE Cucumis sativus
82000
-
4 * 82000, SDS-PAGE Arabidopsis thaliana
350000
-
gel filtration Cucumis sativus
350000
-
gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Cucumis sativus the enzyme might be involved in not only catalysis of the N-terminal hydrolysis of Nalpha-acylpeptides but also the elimination of glycated proteins ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q84LM4
-
-
Cucumis sativus
-
L. suyo
-

Purification (Commentary)

Purification (Comment) Organism
-
Cucumis sativus
-
Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Cucumis sativus
-
shoot
-
Cucumis sativus
-
shoot
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.9
-
-
Cucumis sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Ala-Ala + H2O
-
Cucumis sativus acetyl-Ala + Ala
-
?
acetyl-Ala-Ala + H2O
-
Arabidopsis thaliana acetyl-Ala + Ala
-
?
Acetyl-Ala-Ala-Ala + H2O native enzyme shows 147% of the activity compared to acetyl-Ala-Ala as substrate Cucumis sativus Acetyl-Ala + Ala-Ala
-
?
Acetyl-Ala-Ala-Ala + H2O native enzyme shows 81% of the activity compared to acetyl-Ala-Ala as substrate Arabidopsis thaliana Acetyl-Ala + Ala-Ala
-
?
acetyl-Ala-Ala-Ala-Ala + H2O native enzyme shows 126% of the activity compared to acetyl-Ala-Ala as substrate Arabidopsis thaliana acetyl-Ala + Ala-Ala-Ala
-
?
acetyl-Ala-Ala-Ala-Ala + H2O native enzyme shows 85.6% of the activity compared to acetyl-Ala-Ala as substrate Cucumis sativus acetyl-Ala + Ala-Ala-Ala
-
?
acetyl-Ala-Met + H2O native enzyme shows 70.4% of the activity compared to acetyl-Ala-Ala as substrate Cucumis sativus acetyl-Ala + Met
-
?
acetyl-Gly-Gly + H2O native enzyme shows 37% of the activity compared to acetyl-Ala-Ala as substrate Cucumis sativus acetyl-Gly + Gly
-
?
acetyl-Gly-Gly + H2O native enzyme shows 4% of the activity compared to acetyl-Ala-Ala as substrate Arabidopsis thaliana acetyl-Gly + Gly
-
?
acetyl-Gly-Leu + H2O native enzyme shows 30.3% of the activity compared to acetyl-Ala-Ala as substrate Cucumis sativus acetyl-Gly + Leu
-
?
acetyl-Met-Ala + H2O native enzyme shows 4% of the activity compared to acetyl-Ala-Ala as substrate Cucumis sativus acetyl-Met + Ala
-
?
acetyl-Met-Ala + H2O native enzyme shows 45% of the activity compared to acetyl-Ala-Ala as substrate Arabidopsis thaliana acetyl-Met + Ala
-
?
acetyl-Met-Asn + H2O native enzyme shows 34.1% of the activity compared to acetyl-Ala-Ala as substrate Cucumis sativus acetyl-Met + Asn
-
?
acetyl-Met-Glu + H2O native enzyme shows 4.3% of the activity compared to acetyl-Ala-Ala as substrate Cucumis sativus acetyl-Met + Glu
-
?
acetyl-Met-Glu + H2O native enzyme shows 6% of the activity compared to acetyl-Ala-Ala as substrate Arabidopsis thaliana acetyl-Met + Glu
-
?
acetyl-Met-Phe + H2O native enzyme shows 5% of the activity compared to acetyl-Ala-Ala as substrate Arabidopsis thaliana acetyl-Met + Phe
-
?
butyryl-Ala-Ala-Ala + H2O native enzyme shows 33.6% of the activity compared to acetyl-Ala-Ala as substrate Cucumis sativus butyryl-Ala + Ala-Ala
-
?
butyryl-Ala-Ala-Ala + H2O native enzyme shows 77% of the activity compared to acetyl-Ala-Ala as substrate Arabidopsis thaliana butyryl-Ala + Ala-Ala
-
?
formyl-Ala-Ala-Ala + H2O native enzyme shows 62.4% of the activity compared to acetyl-Ala-Ala as substrate Cucumis sativus formyl-Ala + Ala-Ala
-
?
formyl-Ala-Ala-Ala + H2O native enzyme shows 81% of the activity compared to acetyl-Ala-Ala as substrate Arabidopsis thaliana formyl-Ala + Ala-Ala
-
?
formyl-Gly-Val + H2O native enzyme shows 30.3% of the activity compared to acetyl-Ala-Ala as substrate Cucumis sativus formyl-Gly + Val
-
?
glycated ribulose-1,5-diphosphate carboxylase/oxygenase protein + H2O no degradation of the native protein Cucumis sativus ?
-
?
additional information the enzyme might be involved in not only catalysis of the N-terminal hydrolysis of Nalpha-acylpeptides but also the elimination of glycated proteins Cucumis sativus ?
-
?
N-acetyl-Ala-p-nitroanilide + H2O substrates in the order of catalytic efficiency: N-acetyl-Ala-p-nitranilide, N-acetyl-Gly-p-nitranilide, N-acetyl-Met-p-nitranilide Cucumis sativus N-acetyl-Ala + p-nitroaniline
-
?
N-acetyl-Ala-p-nitroanilide + H2O substrates in the order of catalytic efficiency: N-acetyl-Ala-p-nitranilide, N-acetyl-Met-p-nitranilide, N-acetyl-Gly-p-nitranilide Arabidopsis thaliana N-acetyl-Ala + p-nitroaniline
-
?
N-acetyl-Gly-p-nitroanilide + H2O substrates in the order of catalytic efficiency: N-acetyl-Ala-p-nitranilide, N-acetyl-Gly-p-nitranilide, N-acetyl-Met-p-nitranilide Cucumis sativus N-acetyl-Gly + p-nitroaniline
-
?
N-acetyl-Gly-p-nitroanilide + H2O substrates in the order of catalytic efficiency: N-acetyl-Ala-p-nitranilide, N-acetyl-Met-p-nitranilide, N-acetyl-Gly-p-nitranilide Arabidopsis thaliana N-acetyl-Gly + p-nitroaniline
-
?
N-acetyl-Met-p-nitroanilide + H2O substrates in the order of catalytic efficiency: N-acetyl-Ala-p-nitranilide, N-acetyl-Gly-p-nitranilide, N-acetyl-Met-p-nitranilide Cucumis sativus N-acetyl-Met + p-nitroaniline
-
?
N-acetyl-Met-p-nitroanilide + H2O substrates in the order of catalytic efficiency: N-acetyl-Ala-p-nitranilide, N-acetyl-Met-p-nitranilide, N-acetyl-Gly-p-nitranilide Arabidopsis thaliana N-acetyl-Met + p-nitroaniline
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 82000, SDS-PAGE Cucumis sativus
tetramer 4 * 82000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtAARE
-
Arabidopsis thaliana
cAARE
-
Cucumis sativus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Cucumis sativus
37
-
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Cucumis sativus
7
-
-
Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Cucumis sativus isoelectric focusing
-
5.5