Activating Compound | Comment | Organism | Structure |
---|---|---|---|
allopurinol | addition of allopurinol alone and allopurinol plus 5,5-dimethyl-1-pyrroline N-oxide results in increased CPB1 activity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lipopolysaccharide | lipopolysaccharide treatment significantly decreases carboxypeptidase B activity. Administration of 5,5-dimethyl-1-pyrroline N-oxide to LPS-treated mice alleviates this loss of enzyme activity | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
nitrosylation | a post-translational DMPO-nitrone adduct formed with CPB1 in mice treated with a single bolus dose of lipopolysaccharide is detected | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Mus musculus | - |
Synonyms | Comment | Organism |
---|---|---|
Carboxypeptidase B1 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
metabolism | nitrone spin-trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and a combination of immuno-spin trapping and mass spectrometry is used to identify in vivo products of radical reactions in mice. Dose-dependent production of 5,5-dimethyl-1-pyrroline N-oxide-CPB1 adducts are detected in the spleens of mice treated with lipopolysaccharide and also under normal physiological conditions. Treatments with inhibitors and experiments with knock-out mice indicate that xanthine oxidase and endothelial nitric oxide synthase are important sources of the reactive species that lead to CPB1 adduct formation | Mus musculus |