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Literature summary for 3.4.17.2 extracted from
- Kinetic properties of carboxypeptidase B in solutions and crystals (1977), Biochemistry, 16, 3663-3668.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
benzoyl-Gly-Arg | soluble enzyme | Bos taurus |  |
| 0.23 | - |
benzoyl-Gly-Arg | soluble enzyme | Bos taurus | |
| 0.6 | - |
N-(phenylcarbonyl)glycyl-L-arginine | crystallized enzyme | Bos taurus | |
| 3 | - |
benzoyl-Gly-glycylphenyllactic acid | crystallized enzyme | Bos taurus | |
| 8 | - |
benzoyl-Gly-Arg | crystallized enzyme | Bos taurus | |
| 24 | - |
benzoyl-Gly-Gly-Phe | crystallized enzyme | Bos taurus | |
| 40 | - |
benzoyl-Gly-Gly-Phe | soluble enzyme | Bos taurus | |
| 80 | - |
Carbobenzoxy-Gly-Phe | soluble enzyme | Bos taurus | |
| 80 | - |
benzoyl-Gly-glycylphenyllactic acid | soluble enzyme | Bos taurus | |
| 100 | - |
Carbobenzoxy-Gly-Phe | crystallized enzyme | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-Gly-Gly-Phe + H2O | - |
Bos taurus | ? | - |
? | |
| benzoyl-Gly-glycylphenyllactic acid + H2O | - |
Bos taurus | ? | - |
? | |
| carbobenzoxy-Gly-L-Phe + H2O | - |
Bos taurus | ? | - |
? | |
| N-benzoyl-Gly-L-Arg + H2O | - |
Bos taurus | N-benzoyl-Gly + L-Arg | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.333 | - |
benzoyl-Gly-Gly-Phe | crystallized enzyme | Bos taurus | |
| 0.667 | - |
N-(phenylcarbonyl)glycyl-L-arginine | crystallized enzyme | Bos taurus | |
| 2.5 | - |
carbobenbenzoxy-Gly-Phe | crystallized enzyme | Bos taurus | |
| 2.83 | - |
benzoyl-Gly-glycylphenyllactic acid | crystallized enzyme | Bos taurus | |
| 3.67 | - |
benzoyl-Gly-Arg | crystallized enzyme | Bos taurus | |
| 5.5 | - |
benzoyl-Gly-Gly-Phe | soluble enzyme | Bos taurus | |
| 60 | - |
benzoyl-Gly-Arg | soluble enzyme | Bos taurus | |
| 104 | - |
Carbobenzoxy-Gly-Phe | soluble enzyme | Bos taurus | |
| 183 | - |
benzoyl-Gly-Arg | soluble enzyme | Bos taurus | |
| 917 | - |
benzoyl-Gly-glycylphenyllactic acid | soluble enzyme | Bos taurus | |
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