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Literature summary for 3.4.11.25 extracted from

  • Geueke, B.; Heck, T.; Limbach, M.; Nesatyy, V.; Seebach, D.; Kohler, H.P.
    Bacterial beta-peptidyl aminopeptidases with unique substrate specificities for beta-oligopeptides and mixed beta,alpha-oligopeptides (2006), FEBS J., 273, 5261-5272.
    View publication on PubMed
Search for more literature for 3.4.11.25

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition: EDTA (0.1 mM, 1 mM and 10 mM), leupeptin (0.01 mM, 0.1 mM and 1 mM), phenylmethanesulfonyl fluoride (1 mM and 10 mM), bestatin (0.01 mM and 0.1 mM), and 1,10-phenanthroline (1 mM and 10 mM) Sphingosinicella microcystinivorans
additional information no inhibition: EDTA (0.1 mM, 1 mM and 10 mM), leupeptin (0.01 mM, 0.1 mM and 1 mM), phenylmethanesulfonyl fluoride (1 mM and 10 mM), bestatin (0.01 mM and 0.1 mM), and 1,10-phenanthroline (1 mM and 10 mM) Sphingosinicella xenopeptidilytica
Pefabloc SC 0.4 mM and 4 mM, complete inhibition of hydrolysis of beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans
Pefabloc SC 0.4 mM and 4 mM, complete inhibition of hydrolysis of beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4
-
 beta-Ala-p-nitroanilide pH 8.0, 25°C Sphingosinicella microcystinivorans
8.2
-
 beta-Ala-p-nitroanilide pH 8.0, 25°C Sphingosinicella xenopeptidilytica
9
-
 beta-homoVal-beta-homoAla-beta-homoLeu pH 8.0, 25°C Sphingosinicella xenopeptidilytica
20
-
 beta-homoAla-beta-homoLeu pH 8.0, 25°C Sphingosinicella xenopeptidilytica
39
-
 beta-homoVal-beta-homoAla-beta-homoLeu pH 8.0, 25°C Sphingosinicella microcystinivorans
41
-
 beta-homoAla-beta-homoLeu pH 8.0, 25°C Sphingosinicella microcystinivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13144
-
 alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence Sphingosinicella microcystinivorans
13168
-
 alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS Sphingosinicella microcystinivorans
25333
-
 alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence Sphingosinicella microcystinivorans
25465
-
 alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS Sphingosinicella microcystinivorans
130000
-
 gel filtration Sphingosinicella xenopeptidilytica
130000
-
 gel filtration Sphingosinicella microcystinivorans
150200
-
 electrospray ionisation mass spectrometry Sphingosinicella xenopeptidilytica
155800
-
 electrospray ionisation mass spectrometry Sphingosinicella microcystinivorans

Organism

Organism UniProt Comment Textmining
Sphingosinicella microcystinivorans A0MTQ2
-
-
Sphingosinicella microcystinivorans Y2 A0MTQ2
-
-
Sphingosinicella xenopeptidilytica Q52VH2
-
-
Sphingosinicella xenopeptidilytica 3-2W4 Q52VH2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification cleavage of the Y2 BapA preprotein between the conserved residues N275 and S276 Sphingosinicella microcystinivorans

Purification (Commentary)

Purification (Comment) Organism
recombinant Sphingosinicella xenopeptidilytica
recombinant Sphingosinicella microcystinivorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 pH 8.0, 25°C, substrate: beta-Ala-p-nitroanilide Sphingosinicella microcystinivorans
0.62
-
 pH 8.0, 25°C, substrate: beta-Ala-p-nitroanilide Sphingosinicella xenopeptidilytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-Ala-Ile-beta-homoTyr + H2O 0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-Ala + Ile-beta-homoTyr
-
 ?
beta-Ala-Ile-beta-homoTyr + H2O 1.5% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-Ala + Ile-beta-homoTyr
-
 ?
beta-Ala-Ile-beta-homoTyr + H2O 0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica 3-2W4 beta-Ala + Ile-beta-homoTyr
-
 ?
beta-Ala-Ile-beta-homoTyr + H2O 1.5% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans Y2 beta-Ala + Ile-beta-homoTyr
-
 ?
beta-Ala-L-His + H2O i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-Ala + L-His
-
 ?
beta-Ala-L-His + H2O i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-Ala + L-His
-
 ?
beta-Ala-L-His + H2O i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica 3-2W4 beta-Ala + L-His
-
 ?
beta-Ala-L-His + H2O i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans Y2 beta-Ala + L-His
-
 ?
beta-Ala-p-nitroanilide + H2O
-
 Sphingosinicella xenopeptidilytica beta-Ala + p-nitroaniline
-
 ?
beta-Ala-p-nitroanilide + H2O
-
 Sphingosinicella microcystinivorans beta-Ala + p-nitroaniline
-
 ?
beta-Ala-p-nitroanilide + H2O
-
 Sphingosinicella xenopeptidilytica 3-2W4 beta-Ala + p-nitroaniline
-
 ?
beta-Ala-p-nitroanilide + H2O
-
 Sphingosinicella microcystinivorans Y2 beta-Ala + p-nitroaniline
-
 ?
beta-homoAla-beta-homoLeu + H2O preferred substrate Sphingosinicella microcystinivorans beta-homoAla + beta-homoLeu
-
 ?
beta-homoAla-beta-homoLeu + H2O 35% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-homoAla + beta-homoLeu
-
 ?
beta-homoAla-beta-homoLeu + H2O 35% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica 3-2W4 beta-homoAla + beta-homoLeu
-
 ?
beta-homoAla-beta-homoLeu + H2O preferred substrate Sphingosinicella microcystinivorans Y2 beta-homoAla + beta-homoLeu
-
 ?
beta-homoArg-Ile-beta-homoTyr + H2O 0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-homoArg + Ile-beta-homoTyr
-
 ?
beta-homoArg-Ile-beta-homoTyr + H2O 3.4% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoArg + Ile-beta-homoTyr
-
 ?
beta-homoGln-Ile-beta-homoTyr + H2O 0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-homoGln + Ile-beta-homoTyr
-
 ?
beta-homoGln-Ile-beta-homoTyr + H2O 0.3% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoGln + Ile-beta-homoTyr
-
 ?
beta-homoHis-Ile-beta-homoTyr + H2O 0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-homoHis + Ile-beta-homoTyr
-
 ?
beta-homoHis-Ile-beta-homoTyr + H2O 0.4% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoHis + Ile-beta-homoTyr
-
 ?
beta-homoLeu-Ile-beta-homoTyr + H2O 12% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoLeu + Ile-beta-homoTyr
-
 ?
beta-homoLeu-Ile-beta-homoTyr + H2O 61% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-homoLeu + Ile-beta-homoTyr
-
 ?
beta-homoLys-Ile-beta-homoTyr + H2O 0.5% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoLys + Ile-beta-homoTyr
-
 ?
beta-homoLys-Ile-beta-homoTyr + H2O 0.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-homoLys + Ile-beta-homoTyr
-
 ?
beta-homoPhe-Ile-beta-homoTyr + H2O 15% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoPhe + Ile-beta-homoTyr
-
 ?
beta-homoPhe-Ile-beta-homoTyr + H2O 22% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-homoPhe + Ile-beta-homoTyr
-
 ?
beta-homoSer-Ile-beta-homoTyr + H2O 13% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoSer + Ile-beta-homoTyr
-
 ?
beta-homoSer-Ile-beta-homoTyr + H2O 3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-homoSer + Ile-beta-homoTyr
-
 ?
beta-homoThr-Ile-beta-homoTyr + H2O 1.6% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoThr + Ile-beta-homoTyr
-
 ?
beta-homoThr-Ile-beta-homoTyr + H2O 2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-homoThr + Ile-beta-homoTyr
-
 ?
beta-homoTrp-Ile-beta-homoTyr + H2O 1.3% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoTrp + Ile-beta-homoTyr
-
 ?
beta-homoTrp-Ile-beta-homoTyr + H2O 1.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-homoTrp + Ile-beta-homoTyr
-
 ?
beta-homoTyr-Ile-beta-homoTyr + H2O 15% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-homoTyr + Ile-beta-homoTyr
-
 ?
beta-homoTyr-Ile-beta-homoTyr + H2O 7% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoTyr + Ile-beta-homoTyr
-
 ?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O preferred substrate Sphingosinicella xenopeptidilytica beta-homoVal + beta-homoAla-beta-homoLeu
-
 ?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O 27% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoVal + beta-homoAla-beta-homoLeu
-
 ?
beta-homoVal-Ile-beta-homoTyr + H2O 15% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoVal + Ile-beta-homoTyr
-
 ?
beta-homoVal-Ile-beta-homoTyr + H2O 32% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica beta-homoVal + Ile-beta-homoTyr
-
 ?
D-beta-homoVal-Ile-beta-homoTyr + H2O the rate of cleavage is slower by factor 28 as compared to the L-enantiomer. 0.5% of the activity with beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans D-beta-homoVal + Ile-beta-homoTyr
-
 ?
D-beta-homoVal-Ile-beta-homoTyr + H2O the rate of cleavage is slower by factor 35 as compared to the L-enantiomer. 0.9% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu Sphingosinicella xenopeptidilytica D-beta-homoVal + Ile-beta-homoTyr
-
 ?
additional information the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide Sphingosinicella microcystinivorans ?
-
 ?
additional information the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide Sphingosinicella xenopeptidilytica ?
-
 ?
additional information the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide Sphingosinicella xenopeptidilytica 3-2W4 ?
-
 ?
additional information the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide Sphingosinicella microcystinivorans Y2 ?
-
 ?

Subunits

Subunits Comment Organism
octamer alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence Sphingosinicella microcystinivorans
octamer alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS Sphingosinicella microcystinivorans

Synonyms

Synonyms Comment Organism
3-2W4 BapA
-
 Sphingosinicella xenopeptidilytica
Y2 BapA
-
 Sphingosinicella microcystinivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Sphingosinicella xenopeptidilytica
25
-
 assay at Sphingosinicella microcystinivorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life: 1 h Sphingosinicella microcystinivorans
60
-
 24 h, stable Sphingosinicella xenopeptidilytica
70
-
 5 min, complete inactivation Sphingosinicella microcystinivorans
70
-
 half-life: 26 min Sphingosinicella xenopeptidilytica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 beta-Ala-p-nitroanilide pH 8.0, 25°C Sphingosinicella microcystinivorans
0.75
-
 beta-Ala-p-nitroanilide pH 8.0, 25°C Sphingosinicella xenopeptidilytica
4.3
-
 beta-homoVal-beta-homoAla-beta-homoLeu pH 8.0, 25°C Sphingosinicella microcystinivorans
6
-
 beta-homoVal-beta-homoAla-beta-homoLeu pH 8.0, 25°C Sphingosinicella xenopeptidilytica
12
-
 beta-homoAla-beta-homoLeu pH 8.0, 25°C Sphingosinicella xenopeptidilytica
60
-
 beta-homoAla-beta-homoLeu pH 8.0, 25°C Sphingosinicella microcystinivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Sphingosinicella xenopeptidilytica
8
-
 assay at Sphingosinicella microcystinivorans
8 9
-
 Sphingosinicella xenopeptidilytica
10
-
-
 Sphingosinicella microcystinivorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0085
-
 beta-Ala-p-nitroanilide pH 8.0, 25°C Sphingosinicella xenopeptidilytica
0.017
-
 beta-Ala-p-nitroanilide pH 8.0, 25°C Sphingosinicella microcystinivorans
0.029
-
 beta-Ala-L-His pH 8.0, 25°C Sphingosinicella microcystinivorans
0.092
-
 beta-Ala-L-His pH 8.0, 25°C Sphingosinicella xenopeptidilytica
0.11
-
 beta-homoVal-beta-homoAla-beta-homoLeu pH 8.0, 25°C Sphingosinicella microcystinivorans
0.59
-
 beta-homoAla-beta-homoLeu pH 8.0, 25°C Sphingosinicella xenopeptidilytica
0.67
-
 beta-homoVal-beta-homoAla-beta-homoLeu pH 8.0, 25°C Sphingosinicella xenopeptidilytica
1.5
-
 beta-homoAla-beta-homoLeu pH 8.0, 25°C Sphingosinicella microcystinivorans