Information on EC 3.4.11.25 - beta-peptidyl aminopeptidase

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The expected taxonomic range for this enzyme is: Sphingosinicella

EC NUMBER
COMMENTARY
3.4.11.25
-
RECOMMENDED NAME
GeneOntology No.
beta-peptidyl aminopeptidase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
cleaves N-terminal beta-homoamino acids from peptides composed of 2 to 6 amino acids
show the reaction diagram
-
-
-
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3-2W4 BapA
Sphingosinicella xenopeptidilytica 3-2W4
Q52VH2
-
-
Y2 BapA
Sphingosinicella microcystinivorans Y2
Q27YF8
-
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Sphingosinicella microcystinivorans Y2
strain Y2
UniProt
Manually annotated by BRENDA team
Sphingosinicella xenopeptidilytica 3-2W4
strain 3-2W4
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
Sphingosinicella xenopeptidilytica strain 3-2W4 is able to utilize the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu as sole carbon and energy sources
metabolism
Sphingosinicella xenopeptidilytica 3-2W4
-
Sphingosinicella xenopeptidilytica strain 3-2W4 is able to utilize the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu as sole carbon and energy sources
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
show the reaction diagram
Q52VH2
completely degraded to the corresponding beta-homoamino acids within 312 h
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
show the reaction diagram
Q27YF8
completely degraded to the corresponding beta-homoamino acids within 312 h
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
Q52VH2
completely degraded to the corresponding beta-homoamino acids within 312 h
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
show the reaction diagram
Sphingosinicella microcystinivorans Y2
Q27YF8
completely degraded to the corresponding beta-homoamino acids within 312 h
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
-
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
-
1.5% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
Q52VH2
hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
Q27YF8
hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
Q52VH2
hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
-
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
Sphingosinicella microcystinivorans Y2
Q27YF8
hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
Sphingosinicella microcystinivorans Y2
-
1.5% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
-
i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
-
i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
Q52VH2
i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
Q27YF8
i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
Q52VH2
i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
-
i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
Sphingosinicella microcystinivorans Y2
Q27YF8
i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
Sphingosinicella microcystinivorans Y2
-
i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
Q52VH2
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
Q27YF8
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
-
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
Q52VH2
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
-
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
Sphingosinicella microcystinivorans Y2
Q27YF8
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
Sphingosinicella microcystinivorans Y2
-
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
show the reaction diagram
-
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
show the reaction diagram
Q27YF8
preferred substrate
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
show the reaction diagram
Q52VH2
hydrolysis at 31% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
-
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla-beta-homoLeu
show the reaction diagram
-
preferred substrate
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla-beta-homoLeu
show the reaction diagram
Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
-
35% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla-beta-homoLeu
show the reaction diagram
Sphingosinicella microcystinivorans Y2
-
preferred substrate
-
-
?
beta-homoAla-p-nitroanilide + H2O
beta-homoAla + p-nitroaniline
show the reaction diagram
Q52VH2
-
-
-
?
beta-homoAla-p-nitroanilide + H2O
beta-homoAla + p-nitroaniline
show the reaction diagram
Q27YF8
-
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
show the reaction diagram
-
0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
show the reaction diagram
-
3.4% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
show the reaction diagram
Q52VH2
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
show the reaction diagram
Q27YF8
hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
show the reaction diagram
-
0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
show the reaction diagram
-
0.3% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
show the reaction diagram
Q52VH2
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
show the reaction diagram
Q27YF8
hydrolysis at 0.25% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis + Ile-beta-homoTyr
show the reaction diagram
-
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis + Ile-beta-homoTyr
show the reaction diagram
-
0.4% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis-Ile-beta-homoTyr
show the reaction diagram
Q52VH2
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis-Ile-beta-homoTyr
show the reaction diagram
Q27YF8
hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
show the reaction diagram
-
12% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
show the reaction diagram
-
61% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
show the reaction diagram
Q27YF8
hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
show the reaction diagram
Q52VH2
hydrolysis at 54% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
show the reaction diagram
-
0.5% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
show the reaction diagram
-
0.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
show the reaction diagram
Q27YF8
hydrolysis at 0.48% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
show the reaction diagram
Q52VH2
hydrolysis at 0.5% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTy
show the reaction diagram
-
15% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTy
show the reaction diagram
-
22% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTyr
show the reaction diagram
Q27YF8
hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTyr
show the reaction diagram
Q52VH2
hydrolysis at 19% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoPhe-p-nitroanilide + H2O
beta-homoPhe + p-nitroaniline
show the reaction diagram
Q52VH2
-
-
-
?
beta-homoPhe-p-nitroanilide + H2O
beta-homoPhe + p-nitroaniline
show the reaction diagram
Q27YF8
-
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
show the reaction diagram
-
13% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
show the reaction diagram
-
3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
show the reaction diagram
Q27YF8
hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
show the reaction diagram
Q52VH2
hydrolysis at 2.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
show the reaction diagram
-
1.6% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
show the reaction diagram
-
2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
show the reaction diagram
Q27YF8
hydrolysis at 1.6% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
show the reaction diagram
Q52VH2
hydrolysis at 1.9% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
show the reaction diagram
-
1.3% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
show the reaction diagram
-
1.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
show the reaction diagram
Q27YF8
hydrolysis at 1.2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
show the reaction diagram
Q52VH2
hydrolysis at 1.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
show the reaction diagram
-
15% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
show the reaction diagram
-
7% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
show the reaction diagram
Q52VH2
hydrolysis at 13% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
show the reaction diagram
Q27YF8
hydrolysis at 7% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
-
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
-
preferred substrate
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
-
27% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
Q27YF8
hydrolysis at 27% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
Q52VH2
hydrolysis at 89% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
-
-
-
-
?
beta-homoVal-beta-homoArg-beta-homoArg + H2O
beta-homoVal + beta-homoArg-beta-homoArg
show the reaction diagram
Q27YF8
hydrolysis at 0.2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoArg-beta-homoArg + H2O
beta-homoVal + beta-homoArg-beta-homoArg
show the reaction diagram
Q52VH2
hydrolysis at 1.4% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O
beta-homoVal + beta-homoIle + beta-homoTyr
show the reaction diagram
Q52VH2
completely hydrolyzed. Hydrolysis at 15% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O
beta-homoVal + beta-homoIle + beta-homoTyr
show the reaction diagram
Q27YF8
completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-beta-homoIle-Tyr + H2O
beta-homoVal + beta-homoIle + Tyr
show the reaction diagram
Q52VH2
completely hydrolyzed. Hydrolysis at 20% compared to hydrolysis of beta-(3-homoVal)-Ile-Tyr
-
-
?
beta-homoVal-beta-homoIle-Tyr + H2O
beta-homoVal + beta-homoIle + Tyr
show the reaction diagram
Q27YF8
completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
-
15% of the activity with beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
-
32% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
Q27YF8
only the N-terminal beta-homoVal is released. Hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
Q52VH2
only the N-terminal beta-homoVal is released. Hydrolysis at 28% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
beta-homoVal-Ile-Tyr + H2O
beta-homoVal + Ile-Tyr
show the reaction diagram
Q27YF8
only the N-terminal beta-homoVal is released. Hydrolysis at 32% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
Q27YF8
hydrolysis at 0.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
Q52VH2
hydrolysis at 0.8% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
-
the rate of cleavage is slower by factor 28 as compared to the L-enantiomer. 0.5% of the activity with beta-homoAla-beta-homoLeu
-
-
?
Val-Ile-Tyr + H2O
beta-homoVal + Ile-Tyr
show the reaction diagram
Q52VH2
only the N-terminal beta-homoVal is released. Preferred substrate
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
-
the rate of cleavage is slower by factor 35 as compared to the L-enantiomer. 0.9% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
-
?
additional information
?
-
Q52VH2
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
-
additional information
?
-
Q27YF8
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
-
additional information
?
-
-
in the reverse direction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
-
additional information
?
-
-
in the reverse reaction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
-
additional information
?
-
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide
-
-
-
additional information
?
-
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide
-
-
-
additional information
?
-
Sphingosinicella xenopeptidilytica 3-2W4
Q52VH2
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
-
additional information
?
-
Sphingosinicella xenopeptidilytica 3-2W4
-
in the reverse reaction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
-
additional information
?
-
Sphingosinicella xenopeptidilytica 3-2W4
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide
-
-
-
additional information
?
-
Sphingosinicella microcystinivorans Y2
Q27YF8
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
-
additional information
?
-
Sphingosinicella microcystinivorans Y2
-
in the reverse direction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
-
additional information
?
-
Sphingosinicella microcystinivorans Y2
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2 or CuCl2
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Pefabloc SC
-
0.4 mM and 4 mM, complete inhibition of hydrolysis of beta-homoVal-beta-homoAla-beta-homoLeu
additional information
-
no inhibition: EDTA (0.1 mM, 1 mM and 10 mM), leupeptin (0.01 mM, 0.1 mM and 1 mM), phenylmethanesulfonyl fluoride (1 mM and 10 mM), bestatin (0.01 mM and 0.1 mM), and 1,10-phenanthroline (1 mM and 10 mM)
-
additional information
-
no inhibitory effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2, CuCl2, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
4.4
-
beta-Ala-p-nitroanilide
-
pH 8.0, 25C
8.1
-
beta-Ala-p-nitroanilide
-
pH 8.0, 25C
8.2
-
beta-Ala-p-nitroanilide
-
pH 8.0, 25C
20
-
beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
41
-
beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
0.7
-
beta-homoAla-p-nitroanilide
-
pH 8.0, 25C
0.76
-
beta-homoAla-p-nitroanilide
-
pH 8.0, 25C
1.23
-
beta-homoPhe-p-nitroanilide
-
pH 8.0, 25C
1.45
-
beta-homoPhe-p-nitroanilide
-
pH 8.0, 25C
9
-
beta-homoVal-beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
39
-
beta-homoVal-beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.13
-
beta-Ala-p-nitroanilide
-
pH 8.0, 25C
0.75
-
beta-Ala-p-nitroanilide
-
pH 8.0, 25C
12
-
beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
60
-
beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
2.89
-
beta-homoAla-p-nitroanilide
-
pH 8.0, 25C
13.2
-
beta-homoAla-p-nitroanilide
-
pH 8.0, 25C
0.22
-
beta-homoPhe-p-nitroanilide
-
pH 8.0, 25C
30.2
-
beta-homoPhe-p-nitroanilide
-
pH 8.0, 25C
4.3
-
beta-homoVal-beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
6
-
beta-homoVal-beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.029
-
beta-Ala-L-His
-
pH 8.0, 25C
257054
0.092
-
beta-Ala-L-His
-
pH 8.0, 25C
257054
0.0085
-
beta-Ala-p-nitroanilide
-
pH 8.0, 25C
302477
0.017
-
beta-Ala-p-nitroanilide
-
pH 8.0, 25C
302477
0.029
-
beta-Ala-p-nitroanilide
-
pH 8.0, 25C
302477
0.092
-
beta-Ala-p-nitroanilide
-
pH 8.0, 25C
302477
0.59
-
beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
302481
1.5
-
beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
302481
3.8
-
beta-homoAla-p-nitroanilide
-
pH 8.0, 25C
302478
18.7
-
beta-homoAla-p-nitroanilide
-
pH 8.0, 25C
302478
0.154
-
beta-homoPhe-p-nitroanilide
-
pH 8.0, 25C
302479
24.6
-
beta-homoPhe-p-nitroanilide
-
pH 8.0, 25C
302479
0.11
-
beta-homoVal-beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
302480
0.67
-
beta-homoVal-beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
302480
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.2
-
-
pH 8.0, 25C, substrate: beta-Ala-p-nitroanilide
0.62
-
-
pH 8.0, 25C, substrate: beta-Ala-p-nitroanilide
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
150200
-
-
electrospray ionisation mass spectrometry
155800
-
-
electrospray ionisation mass spectrometry
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 12000 + x * 27000, SDS-PAGE
?
Sphingosinicella xenopeptidilytica 3-2W4
-
x * 12000 + x * 27000, SDS-PAGE
-
octamer
-
alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence; alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS
octamer
Sphingosinicella microcystinivorans Y2
-
alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence; alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
proteolytic modification
-
cleavage of the Y2 BapA preprotein between the conserved residues N275 and S276
proteolytic modification
Sphingosinicella microcystinivorans Y2
-
cleavage of the Y2 BapA preprotein between the conserved residues N275 and S276
-
proteolytic modification
-
the gene encodes a beta-peptidyl aminopeptidase (BapA) of 402 amino acids that is synthesized as preprotein with a signal sequence of 29 amino acids. The enzyme is cleaved into two subunits (residues 30 to 278 and 279 to 402)
proteolytic modification
Sphingosinicella xenopeptidilytica 3-2W4
-
the gene encodes a beta-peptidyl aminopeptidase (BapA) of 402 amino acids that is synthesized as preprotein with a signal sequence of 29 amino acids. The enzyme is cleaved into two subunits (residues 30 to 278 and 279 to 402)
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
70
-
-
5 min, complete inactivation
70
-
-
half-life: 26 min
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
synthesis
-
attachment of a beta-amino acid to the N-terminus of a natural alpha-peptide. N-terminal beta-amino acid residues may be considered as protective groups against proteolytic enzymes in vitro and in vivo
synthesis
Sphingosinicella microcystinivorans Y2
-
attachment of a beta-amino acid to the N-terminus of a natural alpha-peptide. N-terminal beta-amino acid residues may be considered as protective groups against proteolytic enzymes in vitro and in vivo
-
synthesis
-
attachment of a beta-amino acid to the N-terminus of a natural alpha-peptide. N-terminal beta-amino acid residues may be considered as protective groups against proteolytic enzymes in vitro and in vivo
synthesis
Sphingosinicella xenopeptidilytica 3-2W4
-
attachment of a beta-amino acid to the N-terminus of a natural alpha-peptide. N-terminal beta-amino acid residues may be considered as protective groups against proteolytic enzymes in vitro and in vivo
-