Information on EC 3.4.11.25 - beta-peptidyl aminopeptidase

New: Word Map on EC 3.4.11.25
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY
3.4.11.25
-
RECOMMENDED NAME
GeneOntology No.
beta-peptidyl aminopeptidase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cleaves N-terminal beta-homoamino acids from peptides composed of 2 to 6 amino acids
show the reaction diagram
-
-
-
-
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3-2W4 BapA
Sphingosinicella xenopeptidilytica 3-2W4
-
-
Y2 BapA
Sphingosinicella microcystinivorans Y2
-
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Sphingosinicella microcystinivorans Y2
strain Y2
UniProt
Manually annotated by BRENDA team
Sphingosinicella xenopeptidilytica 3-2W4
strain 3-2W4
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
Sphingosinicella xenopeptidilytica strain 3-2W4 is able to utilize the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu as sole carbon and energy sources
metabolism
Sphingosinicella xenopeptidilytica 3-2W4
-
Sphingosinicella xenopeptidilytica strain 3-2W4 is able to utilize the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu as sole carbon and energy sources
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
show the reaction diagram
completely degraded to the corresponding beta-homoamino acids within 312 h
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
show the reaction diagram
completely degraded to the corresponding beta-homoamino acids within 312 h
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
completely degraded to the corresponding beta-homoamino acids within 312 h
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
show the reaction diagram
Sphingosinicella microcystinivorans Y2
completely degraded to the corresponding beta-homoamino acids within 312 h
-
?
2 beta-homoGly 4-nitroanilide
beta-homoGly-beta-homoGly 4-nitroanilide + 4-nitroaniline
show the reaction diagram
-
product of mutant S237C, synthesized efficiently without degradation
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
-
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
-
1.5% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
-
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
Sphingosinicella microcystinivorans Y2
hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
Sphingosinicella microcystinivorans Y2
-
1.5% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
-
i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
-
i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
-
i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
Sphingosinicella microcystinivorans Y2
i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
Sphingosinicella microcystinivorans Y2
-
i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
-
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
Sphingosinicella microcystinivorans Y2
-
-
?
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
Sphingosinicella microcystinivorans Y2
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
show the reaction diagram
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
show the reaction diagram
preferred substrate
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
show the reaction diagram
hydrolysis at 31% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla-beta-homoLeu
show the reaction diagram
-
preferred substrate
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla-beta-homoLeu
show the reaction diagram
Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
-
35% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-homoAla-beta-homoLeu + H2O
beta-homoAla-beta-homoLeu
show the reaction diagram
Sphingosinicella microcystinivorans Y2
-
preferred substrate
-
?
beta-homoAla-p-nitroanilide + H2O
beta-homoAla + p-nitroaniline
show the reaction diagram
-
-
?
beta-homoAla-p-nitroanilide + H2O
beta-homoAla + p-nitroaniline
show the reaction diagram
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
show the reaction diagram
-
0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
show the reaction diagram
-
3.4% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
show the reaction diagram
-
0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
show the reaction diagram
-
0.3% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 0.25% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoGly 4-nitroanilide + H2O
beta-homoGly + 4-nitroaniline
show the reaction diagram
-
wild-type enzyme shows beta-homoGly-beta-homoGly-4-nitroanilide synthetic activity, but the product vanishes in a few min and converts to free beta-homoGly
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis + Ile-beta-homoTyr
show the reaction diagram
-
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis + Ile-beta-homoTyr
show the reaction diagram
-
0.4% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis-Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis-Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
show the reaction diagram
-
12% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
show the reaction diagram
-
61% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 54% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
show the reaction diagram
-
0.5% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
show the reaction diagram
-
0.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 0.48% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 0.5% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTy
show the reaction diagram
-
15% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTy
show the reaction diagram
-
22% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 19% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoPhe-p-nitroanilide + H2O
beta-homoPhe + p-nitroaniline
show the reaction diagram
-
-
?
beta-homoPhe-p-nitroanilide + H2O
beta-homoPhe + p-nitroaniline
show the reaction diagram
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
show the reaction diagram
-
13% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
show the reaction diagram
-
3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 2.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
show the reaction diagram
-
1.6% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
show the reaction diagram
-
2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 1.6% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 1.9% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
show the reaction diagram
-
1.3% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
show the reaction diagram
-
1.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 1.2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 1.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
show the reaction diagram
-
15% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
show the reaction diagram
-
7% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 13% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 7% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
-
preferred substrate
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
-
27% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
hydrolysis at 27% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
hydrolysis at 89% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
Sphingosinicella xenopeptidilytica 3-2W4
-
-
-
?
beta-homoVal-beta-homoArg-beta-homoArg + H2O
beta-homoVal + beta-homoArg-beta-homoArg
show the reaction diagram
hydrolysis at 0.2% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoVal-beta-homoArg-beta-homoArg + H2O
beta-homoVal + beta-homoArg-beta-homoArg
show the reaction diagram
hydrolysis at 1.4% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O
beta-homoVal + beta-homoIle + beta-homoTyr
show the reaction diagram
completely hydrolyzed. Hydrolysis at 15% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O
beta-homoVal + beta-homoIle + beta-homoTyr
show the reaction diagram
completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoVal-beta-homoIle-Tyr + H2O
beta-homoVal + beta-homoIle + Tyr
show the reaction diagram
completely hydrolyzed. Hydrolysis at 20% compared to hydrolysis of beta-(3-homoVal)-Ile-Tyr
-
?
beta-homoVal-beta-homoIle-Tyr + H2O
beta-homoVal + beta-homoIle + Tyr
show the reaction diagram
completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
-
15% of the activity with beta-homoAla-beta-homoLeu
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
-
32% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
only the N-terminal beta-homoVal is released. Hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
only the N-terminal beta-homoVal is released. Hydrolysis at 28% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
beta-homoVal-Ile-Tyr + H2O
beta-homoVal + Ile-Tyr
show the reaction diagram
only the N-terminal beta-homoVal is released. Hydrolysis at 32% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 0.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
hydrolysis at 0.8% compared to hydrolysis of beta-homoVal-Ile-Tyr
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
-
the rate of cleavage is slower by factor 28 as compared to the L-enantiomer. 0.5% of the activity with beta-homoAla-beta-homoLeu
-
?
Val-Ile-Tyr + H2O
beta-homoVal + Ile-Tyr
show the reaction diagram
only the N-terminal beta-homoVal is released. Preferred substrate
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
-
the rate of cleavage is slower by factor 35 as compared to the L-enantiomer. 0.9% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
-
?
additional information
?
-
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
-
additional information
?
-
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
-
additional information
?
-
-
in the reverse direction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
-
additional information
?
-
-
in the reverse reaction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
-
additional information
?
-
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide
-
-
-
additional information
?
-
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide
-
-
-
additional information
?
-
Sphingosinicella xenopeptidilytica 3-2W4
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
-
additional information
?
-
Sphingosinicella xenopeptidilytica 3-2W4
-
in the reverse reaction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
-
additional information
?
-
Sphingosinicella xenopeptidilytica 3-2W4
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide
-
-
-
additional information
?
-
Sphingosinicella microcystinivorans Y2
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
-
-
-
additional information
?
-
Sphingosinicella microcystinivorans Y2
-
in the reverse direction the enzyme catalyzes the oligomerization of beta-amino acids and the synthesis of mixed peptides with N-terminal beta-amino acid residues. As substrates the beta-homoamino acid derivatives beta-Ala-p-nitroanilide, beta-homoAla-p-nitroanilide, (R)-beta-homoAla-p-nitroanilide, beta-homoPhe-p-nitroanilide, (R)-beta-homoPhe-p-nitroanilide, and beta-homoLeu-p-nitroanilide are utilized
-
-
-
additional information
?
-
Sphingosinicella microcystinivorans Y2
-
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2 or CuCl2
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Pefabloc SC
-
0.4 mM and 4 mM, complete inhibition of hydrolysis of beta-homoVal-beta-homoAla-beta-homoLeu
additional information
-
no inhibition: EDTA (0.1 mM, 1 mM and 10 mM), leupeptin (0.01 mM, 0.1 mM and 1 mM), phenylmethanesulfonyl fluoride (1 mM and 10 mM), bestatin (0.01 mM and 0.1 mM), and 1,10-phenanthroline (1 mM and 10 mM)
-
additional information
-
no inhibitory effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2, CuCl2, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4.4
beta-Ala-p-nitroanilide
-
pH 8.0, 25C
8.1
beta-Ala-p-nitroanilide
-
pH 8.0, 25C
8.2
beta-Ala-p-nitroanilide
-
pH 8.0, 25C
20
beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
41
beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
0.7
beta-homoAla-p-nitroanilide
-
pH 8.0, 25C
0.76
beta-homoAla-p-nitroanilide
-
pH 8.0, 25C
0.0073
beta-homoGly 4-nitroanilide
wild-type, pH 9.0, 25C
-
0.56
beta-homoGly 4-nitroanilide
wild-type, pH 6.0, 25C
-
0.72
beta-homoGly 4-nitroanilide
mutant S237C, pH 9.0, 25C
-
1.23
beta-homoPhe-p-nitroanilide
-
pH 8.0, 25C
1.45
beta-homoPhe-p-nitroanilide
-
pH 8.0, 25C
9
beta-homoVal-beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
39
beta-homoVal-beta-homoAla-beta-homoLeu
-
pH 8.0, 25C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.13
beta-Ala-p-nitroanilide
Sphingosinicella microcystinivorans
-
pH 8.0, 25C
0.75
beta-Ala-p-nitroanilide
Sphingosinicella xenopeptidilytica
-
pH 8.0, 25C
12
beta-homoAla-beta-homoLeu
Sphingosinicella xenopeptidilytica
-
pH 8.0, 25C
60
beta-homoAla-beta-homoLeu
Sphingosinicella microcystinivorans
-
pH 8.0, 25C
2.89
beta-homoAla-p-nitroanilide
Sphingosinicella microcystinivorans
-
pH 8.0, 25C
13.2
beta-homoAla-p-nitroanilide
Sphingosinicella xenopeptidilytica
-
pH 8.0, 25C
1090
beta-homoGly 4-nitroanilide
Pseudomonas aeruginosa
Q9I3M6
wild-type, pH 9.0, 25C
-
1410
beta-homoGly 4-nitroanilide
Pseudomonas aeruginosa
Q9I3M6
wild-type, pH 6.0, 25C
-
4540
beta-homoGly 4-nitroanilide
Pseudomonas aeruginosa
Q9I3M6
mutant S237C, pH 9.0, 25C
-
0.22
beta-homoPhe-p-nitroanilide
Sphingosinicella microcystinivorans
-
pH 8.0, 25C
30.2
beta-homoPhe-p-nitroanilide
Sphingosinicella xenopeptidilytica
-
pH 8.0, 25C
4.3
beta-homoVal-beta-homoAla-beta-homoLeu
Sphingosinicella microcystinivorans
-
pH 8.0, 25C
6
beta-homoVal-beta-homoAla-beta-homoLeu
Sphingosinicella xenopeptidilytica
-
pH 8.0, 25C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.029
beta-Ala-L-His
Sphingosinicella microcystinivorans
-
pH 8.0, 25C
18565
0.092
beta-Ala-L-His
Sphingosinicella xenopeptidilytica
-
pH 8.0, 25C
18565
0.0085
beta-Ala-p-nitroanilide
Sphingosinicella xenopeptidilytica
-
pH 8.0, 25C
8869
0.017
beta-Ala-p-nitroanilide
Sphingosinicella microcystinivorans
-
pH 8.0, 25C
8869
0.029
beta-Ala-p-nitroanilide
Sphingosinicella microcystinivorans
-
pH 8.0, 25C
8869
0.092
beta-Ala-p-nitroanilide
Sphingosinicella xenopeptidilytica
-
pH 8.0, 25C
8869
0.59
beta-homoAla-beta-homoLeu
Sphingosinicella xenopeptidilytica
-
pH 8.0, 25C
10281
1.5
beta-homoAla-beta-homoLeu
Sphingosinicella microcystinivorans
-
pH 8.0, 25C
10281
3.8
beta-homoAla-p-nitroanilide
Sphingosinicella microcystinivorans
-
pH 8.0, 25C
19256
18.7
beta-homoAla-p-nitroanilide
Sphingosinicella xenopeptidilytica
-
pH 8.0, 25C
19256
0.154
beta-homoPhe-p-nitroanilide
Sphingosinicella microcystinivorans
-
pH 8.0, 25C
19257
24.6
beta-homoPhe-p-nitroanilide
Sphingosinicella xenopeptidilytica
-
pH 8.0, 25C
19257
0.11
beta-homoVal-beta-homoAla-beta-homoLeu
Sphingosinicella microcystinivorans
-
pH 8.0, 25C
19258
0.67
beta-homoVal-beta-homoAla-beta-homoLeu
Sphingosinicella xenopeptidilytica
-
pH 8.0, 25C
19258
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.2
-
pH 8.0, 25C, substrate: beta-Ala-p-nitroanilide
0.62
-
pH 8.0, 25C, substrate: beta-Ala-p-nitroanilide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
42000
gel filtration
731203
150200
-
electrospray ionisation mass spectrometry
708467
155800
-
electrospray ionisation mass spectrometry
708467
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 12000 + x * 27000, SDS-PAGE
?
Sphingosinicella xenopeptidilytica 3-2W4
-
x * 12000 + x * 27000, SDS-PAGE
-
dimer
x * 25000, and x * 14000, SDS-PAGE
octamer
-
alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence; alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS
octamer
Sphingosinicella microcystinivorans Y2
-
alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence; alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
proteolytic modification
-
cleavage of the Y2 BapA preprotein between the conserved residues N275 and S276
proteolytic modification
Sphingosinicella microcystinivorans Y2
-
cleavage of the Y2 BapA preprotein between the conserved residues N275 and S276
-
proteolytic modification
-
the gene encodes a beta-peptidyl aminopeptidase (BapA) of 402 amino acids that is synthesized as preprotein with a signal sequence of 29 amino acids. The enzyme is cleaved into two subunits (residues 30 to 278 and 279 to 402)
proteolytic modification
Sphingosinicella xenopeptidilytica 3-2W4
-
the gene encodes a beta-peptidyl aminopeptidase (BapA) of 402 amino acids that is synthesized as preprotein with a signal sequence of 29 amino acids. The enzyme is cleaved into two subunits (residues 30 to 278 and 279 to 402)
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 10
-
731203
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
wild-type, stable up to
731203
60
-
half-life: 1 h
708467
60
-
24 h, stable
708467
60
mutant S237C, stable up to
731203
70
-
5 min, complete inactivation
708467
70
-
half-life: 26 min
708467
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
S237C
mutant synthesizes beta-homoGly-beta-homoGly-4-nitroanilide from beta-homoGly-4-nitroanilide efficiently without degradation, indicating that because of the mutation, the enzyme came to recognize only the amine group as an acyl acceptor instead of water. When using cysteamine as an acyl acceptor, beta-homoGly-cysteamine is synthesized by the mutant only in the reaction using S237C. Mutant S237C is unable to synthesize beta-homoGly-cystamine when using cystamine as an acyl acceptor, although it is synthesized by wild-type
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
-
attachment of a beta-amino acid to the N-terminus of a natural alpha-peptide. N-terminal beta-amino acid residues may be considered as protective groups against proteolytic enzymes in vitro and in vivo
synthesis
Sphingosinicella microcystinivorans Y2
-
attachment of a beta-amino acid to the N-terminus of a natural alpha-peptide. N-terminal beta-amino acid residues may be considered as protective groups against proteolytic enzymes in vitro and in vivo
-
synthesis
-
attachment of a beta-amino acid to the N-terminus of a natural alpha-peptide. N-terminal beta-amino acid residues may be considered as protective groups against proteolytic enzymes in vitro and in vivo
synthesis
Sphingosinicella xenopeptidilytica 3-2W4
-
attachment of a beta-amino acid to the N-terminus of a natural alpha-peptide. N-terminal beta-amino acid residues may be considered as protective groups against proteolytic enzymes in vitro and in vivo
-