Information on EC 3.4.11.25 - beta-peptidyl aminopeptidase

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The expected taxonomic range for this enzyme is: Proteobacteria

EC NUMBER
COMMENTARY hide
3.4.11.25
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RECOMMENDED NAME
GeneOntology No.
beta-peptidyl aminopeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cleaves N-terminal beta-homoamino acids from peptides composed of 2 to 6 amino acids
show the reaction diagram
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
beta-homoAla + beta-homoLys + beta-homoPhe
show the reaction diagram
2 beta-homoGly 4-nitroanilide
beta-homoGly-beta-homoGly 4-nitroanilide + 4-nitroaniline
show the reaction diagram
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product of mutant S237C, synthesized efficiently without degradation
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?
beta-Ala-Ile-beta-homoTyr + H2O
beta-Ala + Ile-beta-homoTyr
show the reaction diagram
beta-Ala-L-His + H2O
beta-Ala + L-His
show the reaction diagram
beta-Ala-p-nitroanilide + H2O
beta-Ala + p-nitroaniline
show the reaction diagram
beta-homoAla-beta-homoLeu + H2O
beta-homoAla + beta-homoLeu
show the reaction diagram
beta-homoAla-beta-homoLeu + H2O
beta-homoAla-beta-homoLeu
show the reaction diagram
beta-homoAla-p-nitroanilide + H2O
beta-homoAla + p-nitroaniline
show the reaction diagram
beta-homoArg-Ile-beta-homoTyr + H2O
beta-homoArg + Ile-beta-homoTyr
show the reaction diagram
beta-homoGln-Ile-beta-homoTyr + H2O
beta-homoGln + Ile-beta-homoTyr
show the reaction diagram
beta-homoGly 4-nitroanilide + H2O
beta-homoGly + 4-nitroaniline
show the reaction diagram
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wild-type enzyme shows beta-homoGly-beta-homoGly-4-nitroanilide synthetic activity, but the product vanishes in a few min and converts to free beta-homoGly
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis + Ile-beta-homoTyr
show the reaction diagram
beta-homoHis-Ile-beta-homoTyr + H2O
beta-homoHis-Ile-beta-homoTyr
show the reaction diagram
beta-homoLeu-Ile-beta-homoTyr + H2O
beta-homoLeu + Ile-beta-homoTyr
show the reaction diagram
beta-homoLys-Ile-beta-homoTyr + H2O
beta-homoLys + Ile-beta-homoTyr
show the reaction diagram
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTy
show the reaction diagram
beta-homoPhe-Ile-beta-homoTyr + H2O
beta-homoPhe + Ile-beta-homoTyr
show the reaction diagram
beta-homoPhe-p-nitroanilide + H2O
beta-homoPhe + p-nitroaniline
show the reaction diagram
beta-homoSer-Ile-beta-homoTyr + H2O
beta-homoSer + Ile-beta-homoTyr
show the reaction diagram
beta-homoThr-Ile-beta-homoTyr + H2O
beta-homoThr + Ile-beta-homoTyr
show the reaction diagram
beta-homoTrp-Ile-beta-homoTyr + H2O
beta-homoTrp + Ile-beta-homoTyr
show the reaction diagram
beta-homoTyr-Ile-beta-homoTyr + H2O
beta-homoTyr + Ile-beta-homoTyr
show the reaction diagram
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
beta-homoVal + beta-homoAla-beta-homoLeu
show the reaction diagram
beta-homoVal-beta-homoArg-beta-homoArg + H2O
beta-homoVal + beta-homoArg-beta-homoArg
show the reaction diagram
beta-homoVal-beta-homoIle-beta-homoTyr + H2O
beta-homoVal + beta-homoIle + beta-homoTyr
show the reaction diagram
beta-homoVal-beta-homoIle-Tyr + H2O
beta-homoVal + beta-homoIle + Tyr
show the reaction diagram
beta-homoVal-Ile-beta-homoTyr + H2O
beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
beta-homoVal-Ile-Tyr + H2O
beta-homoVal + Ile-Tyr
show the reaction diagram
only the N-terminal beta-homoVal is released. Hydrolysis at 32% compared to hydrolysis of beta-homoAla-beta-homoLeu
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-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
D-beta-homoVal + Ile-beta-homoTyr
show the reaction diagram
Val-Ile-Tyr + H2O
beta-homoVal + Ile-Tyr
show the reaction diagram
only the N-terminal beta-homoVal is released. Preferred substrate
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2 or CuCl2
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Pefabloc SC
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.4 - 8.2
beta-Ala-p-nitroanilide
20 - 41
beta-homoAla-beta-homoLeu
0.7 - 0.76
beta-homoAla-p-nitroanilide
0.0073 - 0.72
beta-homoGly 4-nitroanilide
1.23 - 1.45
beta-homoPhe-p-nitroanilide
9 - 39
beta-homoVal-beta-homoAla-beta-homoLeu
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13 - 0.75
beta-Ala-p-nitroanilide
12 - 60
beta-homoAla-beta-homoLeu
2.89 - 13.2
beta-homoAla-p-nitroanilide
1090 - 4540
beta-homoGly 4-nitroanilide
0.22 - 30.2
beta-homoPhe-p-nitroanilide
4.3 - 6
beta-homoVal-beta-homoAla-beta-homoLeu
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.029 - 0.092
beta-Ala-L-His
0.0085 - 0.092
beta-Ala-p-nitroanilide
0.59 - 1.5
beta-homoAla-beta-homoLeu
3.8 - 18.7
beta-homoAla-p-nitroanilide
0.154 - 24.6
beta-homoPhe-p-nitroanilide
0.11 - 0.67
beta-homoVal-beta-homoAla-beta-homoLeu
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.2
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pH 8.0, 25C, substrate: beta-Ala-p-nitroanilide
0.62
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pH 8.0, 25C, substrate: beta-Ala-p-nitroanilide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
gel filtration
130000
150200
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electrospray ionisation mass spectrometry
155800
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electrospray ionisation mass spectrometry
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
x * 25000, and x * 14000, SDS-PAGE
octamer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
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731203
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
wild-type, stable up to
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S237C
mutant synthesizes beta-homoGly-beta-homoGly-4-nitroanilide from beta-homoGly-4-nitroanilide efficiently without degradation, indicating that because of the mutation, the enzyme came to recognize only the amine group as an acyl acceptor instead of water. When using cysteamine as an acyl acceptor, beta-homoGly-cysteamine is synthesized by the mutant only in the reaction using S237C. Mutant S237C is unable to synthesize beta-homoGly-cystamine when using cystamine as an acyl acceptor, although it is synthesized by wild-type
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis