General Stability | Organism |
---|---|
not stable to lyophilization | Hathewaya histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibits in presence of Mg2+ | Hathewaya histolytica | |
Lys(Abz)-Phe-p-nitrobenzyloxy | - |
Hathewaya histolytica | |
p-mercuribenzoate | 1.7 mM, complete inhibition | Hathewaya histolytica | |
Zn2+ | inhibits in presence of Mg2+ | Hathewaya histolytica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates to a lesser extent than Mn2+, no activity in absence of metal ions | Hathewaya histolytica | |
Mn2+ | activates, no activity in absence of metal ions | Hathewaya histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
6 * 56000, SDS-PAGE | Hathewaya histolytica |
340000 | - |
gel filtration | Hathewaya histolytica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hathewaya histolytica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hathewaya histolytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Hathewaya histolytica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Pro-Gly-Pro)n + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro-(Pro-Gly-Pro)n-1 | - |
? | |
(Pro-Gly-Pro)n-methyl ester + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro-(Pro-Gly-Pro)n-methyl ester | - |
? | |
additional information | enzyme can not remove the terminal Pro from poly-L-Pro. The enzyme is strictly an aminopeptidase and has no endopeptidase action on polypeptides and proteins. The amino group of the N-terminal amino acid must be free. Di-, tri- and polypeptide substrates are cleaved and the optimum size accomodated by the active center is four residues. The residues cleaved most rapidly are hydrophobic ones | Hathewaya histolytica | ? | - |
? | |
Pro-Gly-Pro + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 56000, SDS-PAGE | Hathewaya histolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
Lys(Abz)-Phe-p-nitrobenzyloxy | - |
Hathewaya histolytica |