Information on EC 3.4.11.13 - Clostridial aminopeptidase

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The expected taxonomic range for this enzyme is: Hathewaya histolytica

EC NUMBER
COMMENTARY hide
3.4.11.13
-
RECOMMENDED NAME
GeneOntology No.
Clostridial aminopeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Release of any N-terminal amino acid, including proline and hydroxyproline, but no cleavage of Xaa-Pro-
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
59680-69-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Ala)3 + H2O
Ala + Ala-Ala
show the reaction diagram
(Ala)4 + H2O
Ala + (Ala)3
show the reaction diagram
(Ala)4-NH2 + H2O
Ala + (Ala)3-NH2
show the reaction diagram
(Pro-Gly-Pro)4-6 + H2O
Pro + Gly-Pro-(Pro-Gly-Pro)3-5
show the reaction diagram
(Pro-Gly-Pro)4-6-OMe + H2O
Pro + Gly-Pro-(Pro-Gly-Pro)3-5-OMe
show the reaction diagram
(Pro-Gly-Pro)n + H2O
Pro + Gly-Pro-(Pro-Gly-Pro)n-1
show the reaction diagram
-
-
-
-
?
(Pro-Gly-Pro)n-methyl ester + H2O
Pro + Gly-Pro-(Pro-Gly-Pro)n-methyl ester
show the reaction diagram
-
-
-
-
?
Ala-Ala-Phe-Ala + H2O
Ala + Ala-Phe-Ala
show the reaction diagram
Ala-Gly-Gly + H2O
Ala + Gly-Gly
show the reaction diagram
Ala-Lys + H2O
Ala + Lys
show the reaction diagram
Gly-Gly-Phe + H2O
Gly + Gly-Phe
show the reaction diagram
Gly-Gly-Phe-Ala + H2O
Gly + Gly-Phe-Ala
show the reaction diagram
Gly-Phe-Ala + H2O
Gly + Phe-Ala
show the reaction diagram
Hyp-Ala-NH2 + H2O
Hyp-Ala + NH3
show the reaction diagram
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
show the reaction diagram
Leu-Gly-Gly + H2O
Leu + Gly-Gly
show the reaction diagram
Met-Gly-Gly + H2O
Met + Gly-Gly
show the reaction diagram
Met-Met + H2O
Met + Met
show the reaction diagram
Peptides + H2O
?
show the reaction diagram
-
-
-
-
-
Phe-Gly-Gly + H2O
Phe + Gly-Gly
show the reaction diagram
Pro-Gly-Gly + H2O
Pro + Gly-Gly
show the reaction diagram
Pro-Gly-Pro + H2O
Pro + Gly-Pro
show the reaction diagram
Pro-Gly-Pro-Pro + H2O
Pro + Gly-Pro-Pro
show the reaction diagram
Pro-Leu + H2O
Pro + Leu
show the reaction diagram
Pro-Phe + H2O
Pro + Phe
show the reaction diagram
Ser-Gly-Gly + H2O
Ser + Gly-Gly
show the reaction diagram
Val-Gly-Gly + H2O
Val + Gly-Gly
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Peptides + H2O
?
show the reaction diagram
-
-
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
-
above 3-7 mM
CoSO4
-
at high concentration, Co-citrate protects
Lys(Abz)-Phe-p-nitrobenzyloxy
-
-
p-mercuribenzoate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
80
Ala-Gly-Gly
-
-
0.5 - 3
L-leucine 4-nitroanilide
-
-
6.45
Leu-Gly-Gly
-
-
8.4
Phe-Gly-Gly
-
-
40
Pro-Gly-Gly
-
-
20
Pro-Gly-Pro
-
-
20
Pro-Gly-Pro-Pro
-
-
18.5
Val-Gly-Gly
-
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1830
Ala-Gly-Gly
Hathewaya histolytica
-
-
4.2
L-leucine 4-nitroanilide
Hathewaya histolytica
-
-
917
Leu-Gly-Gly
Hathewaya histolytica
-
-
667
Phe-Gly-Gly
Hathewaya histolytica
-
-
1360
Pro-Gly-Gly
Hathewaya histolytica
-
-
561
Pro-Gly-Pro
Hathewaya histolytica
-
-
337
Pro-Gly-Pro-Pro
Hathewaya histolytica
-
-
276
Val-Gly-Gly
Hathewaya histolytica
-
-
additional information
additional information
Hathewaya histolytica
-
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003
Lys(Abz)-Phe-p-nitrobenzyloxy
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
activity of immobilized enzyme 18% of that of soluble enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8 - 8.2
-
substrate Pro-Gly-Pro-Pro
8.2
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 42
-
substrate Pro-Gly-Pro-Pro
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
-
6 * 56000, SDS-PAGE
340000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
inactivated above, immobilized enzyme
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
immobilization increases stability, e.g. to SDS
-
irreversible inactivation at 0.5% M urea
-
not stable to lyophilization
-
very sensitive to traces of Zn2+
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.05 M phosphate buffer or 0.05 M Na-acetate buffer, pH 5.6, 1 mM EDTA, several years, little loss of activity, can be restored by treatment with 2.5 mM dithiothreitol
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4°C, sodium barbital, pH 8.2, 50 mM Na-citrate, 10 mM CoSO4, 10% DMSO, several weeks, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
immobilized enzyme can be used for peptide sequence determination, together with other peptidases, reactive in solutions containing up to 10% DMSO