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Literature summary for 3.4.11.1 extracted from

  • Zhao, T.; Zhang, Y.; Wu, H.; Wang, D.; Chen, Y.; Zhu, M.-J.; Ma, L.Z.
    Extracellular aminopeptidase modulates biofilm development of Pseudomonas aeruginosa by affecting matrix exopolysaccharide and bacterial cell death (2018), Environ. Microbiol. Rep., 10, 583-593 .
    View publication on PubMed
Search for more literature for 3.4.11.1

Application

Application Comment Organism
medicine enzyme PaAP is a therapeutic target for preventing Pseudomonas aeruginosa infection and combating biofilm-related complications Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
gene paaP, cloning in Escherichia coli strain DH5alpha Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information construction of an enzyme deletion mutant DELTApaaP Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas aeruginosa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HZQ8
-
-
Pseudomonas aeruginosa 1C Q9HZQ8
-
-
Pseudomonas aeruginosa ATCC 15692 Q9HZQ8
-
-
Pseudomonas aeruginosa CIP 104116 Q9HZQ8
-
-
Pseudomonas aeruginosa DSM 22644 Q9HZQ8
-
-
Pseudomonas aeruginosa JCM 14847 Q9HZQ8
-
-
Pseudomonas aeruginosa LMG 12228 Q9HZQ8
-
-
Pseudomonas aeruginosa PRS 101 Q9HZQ8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa ATCC 15692 L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa 1C L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa PRS 101 L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa DSM 22644 L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa CIP 104116 L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa LMG 12228 L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Pseudomonas aeruginosa JCM 14847 L-Leu + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
LAP
-
 Pseudomonas aeruginosa
PA2939
-
 Pseudomonas aeruginosa
PaAP
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
 Pseudomonas aeruginosa

Expression

Organism Comment Expression
Pseudomonas aeruginosa enzyme PaAP is a quorum sensing-regulated protein, thus its expression is largely activated at high bacterial density up

General Information

General Information Comment Organism
malfunction lack of extracellular PaAP in biofilms leading to bacterial cell death implies that PaAP might function as a nutrient recycler within a biofilm. Biomass reduction of mutant DELTApaaP grown under a continuous media flow has one day delay compared with the DELTApaaP pellicles grown on static media. Lack of extracellular PaAP triggers bacterial cell death and bacterial cell death in DELTApaaP biofilms is independent of Psl, while the disruption of DELTApaaP biofilms is a result of the degradation of Psl matrix by PslG released from the dead bacteria within biofilms, which in turn led to the dispersion of live bacteria from biofilms into effluents Pseudomonas aeruginosa
physiological function the extracellular aminopeptidase PaAP can release amino acids from the N-terminus of peptide or protein. It modulates biofilm development of Pseudomonas aeruginosa by affecting matrix exopolysaccharide Psl and bacterial cell death. PaAP is a quorum sensing-regulated protein, thus its expression is largely activated at high bacterial density. PaAP might function as a nutrient recycler within a biofilm Pseudomonas aeruginosa