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Literature summary for 3.4.11.1 extracted from
- Purpurin inhibits adipocyte-derived leucine aminopeptidase and angiogenesis in a zebrafish model (2014), Biochem. Biophys. Res. Commun., 450, 561-567 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | knockdown of A-LAP expression by siRNA | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| purpurin | is isolated from the natural product library and inhibits adipocyte-derived leucine aminopeptidase and angiogenesis in a zebrafish model. Purpurin inhibits A-LAP activity in a non-competitive manner showing a strong selectivity toward A-LAP versus another member of M1 family of zinc metallopeptidase, aminopeptidase N (APN). Purpurin can be developed as an anti-angiogenic agent. Purpurin also causes a partial inhibition of extension of the intersegmental vessels (ISVs) from the dorsal aorta | Danio rerio |  |
| purpurin | is isolated from the natural product library and inhibits the proliferation of HUVECs in a dose-dependent manner with an IC50 value of 0.03 mM. The proliferation of other types of cells such as HeLa (cervical carcinoma) and C8161 (melanoma) is not significantly inhibited by purpurin at the same concentration range used for HUVEC. Purpurin selectively inhibits the proliferation of endothelial cells over the other types of mammalian cells. Purpurin does not significantly affect the viability of HUVEC up to 0.02 mM treatment. A marginal decrease in the HUVEC viability is observed between 0.04-0.06 mM, and the significant cytotoxicity is observed at over 0.08 mM treatment with purpurin. The treatment of purpurin does not significantly affect the expression level of A-LAP in HUVEC | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | a zinc metallopeptidase | Homo sapiens | |
| Zn2+ | a zinc metallopeptidase | Danio rerio | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | - |
- |
- |
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipocyte | - |
Homo sapiens | - |
| adipocyte | - |
Danio rerio | - |
| embryo | - |
Danio rerio | - |
| HUVEC cell | human umbilical vein endothelial cell | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Leu 7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
| L-Leu 7-amido-4-methylcoumarin + H2O | - |
Danio rerio | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| A-LAP | - |
Homo sapiens |
| A-LAP | - |
Danio rerio |
| adipocyte-derived leucine aminopeptidase | - |
Homo sapiens |
| adipocyte-derived leucine aminopeptidase | - |
Danio rerio |
| leucine aminopeptidase | - |
Homo sapiens |
| leucine aminopeptidase | - |
Danio rerio |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Danio rerio |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
| 7.4 | - |
assay at | Danio rerio |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics | Homo sapiens | |
| additional information | - |
additional information | inhibition kinetics | Danio rerio |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
pH 7.4, 37°C | Danio rerio | purpurin | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the adipocyte-derived leucine aminopeptidase (A-LAP) is a member of the M1 family of zinc metallopeptidases | Homo sapiens |
| evolution | the adipocyte-derived leucine aminopeptidase (A-LAP) is a member of the M1 family of zinc metallopeptidases | Danio rerio |
| malfunction | knockdown of A-LAP expression by siRNA inhibits HUVEC angiogenesis | Homo sapiens |
| physiological function | adipocyte-derived leucine aminopeptidase (A-LAP) plays a crucial role in angiogenesis | Homo sapiens |
| physiological function | adipocyte-derived leucine aminopeptidase (A-LAP) plays a crucial role in angiogenesis | Danio rerio |
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