Cloned (Comment) | Organism |
---|---|
fpg-L gene is cloned, sequenced and overexpressed in Escherichia coli BH410, expression in Escherichia coli fpg and mutY mutants suppresses their spontaneous GC-TA mutator phenotype | Lactococcus lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains one zinc per enzyme molecule | Lactococcus lactis | |
Zn2+ | contains a single zinc finger motif near the C-terminus | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
active form | Lactococcus lactis |
31300 | - |
1 * 31300, calculated from the amino acid sequence | Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Lactococcus lactis | important role in protecting DNA against oxidative free radicals and reactive oxygen-derived species | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | P42371 | subspecies cremoris ML3 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, overexpressed in Escherichia coli BH410 | Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity | Lactococcus lactis | ? | - |
? | |
DNA + H2O | important role in protecting DNA against oxidative free radicals and reactive oxygen-derived species | Lactococcus lactis | ? | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | 34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue | Lactococcus lactis | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | readily incises dublexes with cytosine, thymine or guanine opposite, but much slower with adenine opposite 7,8-dihydro-8-oxoguanine, 2 activities: DNA-glycosylase and DNA nicking at abasic sites | Lactococcus lactis | 8-hydroxyguanine + DNA | - |
? | |
DNA containing ring-opened N7-methylguanine residues + H2O | - |
Lactococcus lactis | 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Lactococcus lactis |
monomer | 1 * 31300, calculated from the amino acid sequence | Lactococcus lactis |
Synonyms | Comment | Organism |
---|---|---|
Fpg-L | - |
Lactococcus lactis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lactococcus lactis | - |
- |
9 |