Cloned (Comment) | Organism |
---|---|
cloning in Escherichia coli strain DH5alpha, expression of wild-type and mutant enzymes in Escherichia coli strain BL21 RPIL DE3 | Thermobifida fusca |
Protein Variants | Comment | Organism |
---|---|---|
D512A | site-directed mutagenesis, the mutant shows altered polysaccharide substrate specificity compared to the wild-type enzyme | Thermobifida fusca |
L230A | site-directed mutagenesis, the mutation causes slightly increased processivity and increased activity with phosphoric acid-treated cotton over 250%, the mutant shows altered polysaccharide substrate specificity compared to the wild-type enzyme | Thermobifida fusca |
M514A | site-directed mutagenesis, the mutation alters the secondary structure of the enzyme, the mutant shows altered polysaccharide substrate specificity compared to the wild-type enzyme | Thermobifida fusca |
M514Q | site-directed mutagenesis, the mutation alters the secondary structure of the enzyme, the mutant shows altered polysaccharide substrate specificity compared to the wild-type enzyme | Thermobifida fusca |
additional information | mixtures of the Cel6B mutant enzymes and Thermobifida fusca endocellulase Cel5A do not show increased synergism or processivity, and the mutant enzyme which has the highest processivity give the poorest synergism | Thermobifida fusca |
N282A | site-directed mutagenesis, mutation in residue near the tunnel entrance causes a twofold increase in processivity, the mutant shows altered polysaccharide substrate specificity compared to the wild-type enzyme | Thermobifida fusca |
N282D | site-directed mutagenesis, mutation in residue near the tunnel entrance causes a twofold increase in processivity, the mutant shows altered polysaccharide substrate specificity compared to the wild-type enzyme | Thermobifida fusca |
R180A | site-directed mutagenesis, mutation in residue near the tunnel exit causes a twofold increase in processivity, the mutant shows altered polysaccharide substrate specificity compared to the wild-type enzyme | Thermobifida fusca |
R180K | site-directed mutagenesis, mutation in residue near the tunnel exit causes a twofold increase in processivity, the mutant shows altered polysaccharide substrate specificity compared to the wild-type enzyme | Thermobifida fusca |
W464A | site-directed mutagenesis, the mutant shows altered polysaccharide substrate specificity compared to the wild-type enzyme | Thermobifida fusca |
W464Y | site-directed mutagenesis, the mutant shows altered polysaccharide substrate specificity compared to the wild-type enzyme | Thermobifida fusca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + H2O | Thermobifida fusca | cellobiohydrolases act from one end of a cellulose chain and processively cleave off cellobiose as the main product. Processivity and synergism are important properties of cellulases, particularly for hydrolysis of crystalline substrates | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellulose + H2O | cellobiohydrolases act from one end of a cellulose chain and processively cleave off cellobiose as the main product. Processivity and synergism are important properties of cellulases, particularly for hydrolysis of crystalline substrates | Thermobifida fusca | ? | - |
? | |
cellulose + H2O | substrates are phosphoric acid-treated cotton, carboxymethylcellulose, bacterial microcrystalline cellulose, and phosphoric acid-swollen cellulose, as well as 8-mg/ml Whatman no. 1 filter paper, substrate specificity of wild-type and mutant enzymes, overview | Thermobifida fusca | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cel6B | - |
Thermobifida fusca |
cellobiohydrolase | - |
Thermobifida fusca |
exocellulase | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | Thermobifida fusca |