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Literature summary for 3.2.1.87 extracted from

  • Majkowska-Skrobek, G.; Latka, A.; Berisio, R.; Squeglia, F.; Maciejewska, B.; Briers, Y.; Drulis-Kawa, Z.
    Phage-borne depolymerases decrease Klebsiella pneumoniae resistance to innate defense mechanisms (2018), Front. Microbiol., 9, 2517 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.87

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Klebsiella phage KP32

General Stability

General Stability Organism
the enzyme is not resistant against denaturation by SDS at room temperature and against digestion by trypsin Klebsiella phage KP32
the enzyme is resistant against denaturation by SDS at room temperature and against digestion by trypsin Klebsiella phage KP32

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
179400
-
 gel filtration Klebsiella phage KP32
259500
-
 gel filtration Klebsiella phage KP32

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Klebsiella phage KP32 the enzymes have high specificity for the capsular serotypes K3 and K21 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella phage KP32 D1L2X0
-
-
Klebsiella phage KP32 D1L2X1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and gel filtration Klebsiella phage KP32

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzymes have high specificity for the capsular serotypes K3 and K21 Klebsiella phage KP32 ?
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 62000, SDS-PAGE Klebsiella phage KP32
trimer 3 * 83000, SDS-PAGE Klebsiella phage KP32

Synonyms

Synonyms Comment Organism
capsule depolymerase
-
 Klebsiella phage KP32
KP32gp37
-
 Klebsiella phage KP32
KP32gp38
-
 Klebsiella phage KP32

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Klebsiella phage KP32

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
18 50 the enzyme maintains more than 95% of its activity between 18 and 45°C. At 50°C the enzyme activity drops to 16.7% Klebsiella phage KP32
18 50 the enzyme maintains more than 95% of its activity between 18 and 45°C. At 50°C, the enzyme displays more than 90% of its activity Klebsiella phage KP32

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
 melting temperature Klebsiella phage KP32
74
-
 melting temperature Klebsiella phage KP32

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Klebsiella phage KP32

pH Range

pH Minimum pH Maximum Comment Organism
7 9 more than 70% activity between pH 7.0 and 9.0 Klebsiella phage KP32

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 after an incubation for 1 h under acidic conditions (pH 6.0 and below), less than 30% of the original activity is preserved Klebsiella phage KP32

General Information

General Information Comment Organism
physiological function the enzyme decreases Klebsiella pneumoniae resistance to innate defense mechanisms and strongly affects the virulence of Klebsiella pneumoniae with the corresponding K3 and K21 capsular serotypes. The enzyme considerably increases the lifespan of Galleria mellonella larvae infected with Klebsiella pneumoniae in a time- and strain-dependent manner Klebsiella phage KP32