Information on EC 3.2.1.87 - capsular-polysaccharide endo-1,3-alpha-galactosidase

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The expected taxonomic range for this enzyme is: Klebsiella aerogenes

EC NUMBER
COMMENTARY hide
3.2.1.87
-
RECOMMENDED NAME
GeneOntology No.
capsular-polysaccharide endo-1,3-alpha-galactosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Random hydrolysis of (1->3)-alpha-D-galactosidic linkages in Aerobacter aerogenes capsular polysaccharide
show the reaction diagram
hydrolyzes the galactosyl-alpha-1,3-D-galactose linkages only in the complex substrate, bringing about depolymerization
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
Aerobacter-capsular-polysaccharide galactohydrolase
Hydrolyses the galactosyl-alpha-1,3-D-galactose linkages only in the complex substrate, bringing about depolymerization.
CAS REGISTRY NUMBER
COMMENTARY hide
62213-16-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
phage induced
-
-
Manually annotated by BRENDA team
Klebsiella pneumoniae capsular serotype K2
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-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
[D-Gal-(1-3)(D-GlcUA-(1-2))-alpha-D-Man-(1-3)-alpha-D-Gal]n + H2O
D-Gal-(1-3)(D-GlcUA-(1-2))-alpha-D-Man-(1-3)-alpha-D-Gal
show the reaction diagram
additional information
?
-
Klebsiella pneumoniae capsular serotype K2
-
virus K2 hydrolyses capsule of Aerobacter aerogenesis using glucane hydrolase splitting alpha1,3-bond between galactose residues
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
[D-Gal-(1-3)(D-GlcUA-(1-2))-alpha-D-Man-(1-3)-alpha-D-Gal]n + H2O
D-Gal-(1-3)(D-GlcUA-(1-2))-alpha-D-Man-(1-3)-alpha-D-Gal
show the reaction diagram
additional information
?
-
Klebsiella pneumoniae capsular serotype K2
-
virus K2 hydrolyses capsule of Aerobacter aerogenesis using glucane hydrolase splitting alpha1,3-bond between galactose residues
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
metal ion not required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36400
-
heptamer: 4 * 63200 + 3 * 36400 or octamer: 4 * 63200 + 4 * 36400, SDS-PAGE; octamer: 4 * 63200 + 4 * 36400 or heptamer: 4 * 63200 + 3 * 36400, SDS-PAGE
63200
-
heptamer: 4 * 63200 + 3 * 36400 or octamer: 4 * 63200 + 4 * 36400, SDS-PAGE; octamer: 4 * 63200 + 4 * 36400 or heptamer: 4 * 63200 + 3 * 36400, SDS-PAGE
379000
-
sedimentation coefficient, diffusion coefficient
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heptamer
-
heptamer: 4 * 63200 + 3 * 36400 or octamer: 4 * 63200 + 4 * 36400, SDS-PAGE
octamer
-
octamer: 4 * 63200 + 4 * 36400 or heptamer: 4 * 63200 + 3 * 36400, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68
-
52% inactivation after 10 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE